Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”

Acidobacteria represents one of the most dominant bacterial groups across diverse ecosystems. However, insight into their ecology and physiology has been hampered by difficulties in cultivating members of this phylum. Previous cultivation efforts have suggested an important role of trace elements fo...

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Main Authors: Ohana Y. A. Costa, Chidinma Oguejiofor, Daniela Zühlke, Cristine C. Barreto, Christine Wünsche, Katharina Riedel, Eiko E. Kuramae
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-06-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.01227/full
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author Ohana Y. A. Costa
Ohana Y. A. Costa
Chidinma Oguejiofor
Daniela Zühlke
Cristine C. Barreto
Christine Wünsche
Katharina Riedel
Eiko E. Kuramae
Eiko E. Kuramae
author_facet Ohana Y. A. Costa
Ohana Y. A. Costa
Chidinma Oguejiofor
Daniela Zühlke
Cristine C. Barreto
Christine Wünsche
Katharina Riedel
Eiko E. Kuramae
Eiko E. Kuramae
author_sort Ohana Y. A. Costa
collection DOAJ
description Acidobacteria represents one of the most dominant bacterial groups across diverse ecosystems. However, insight into their ecology and physiology has been hampered by difficulties in cultivating members of this phylum. Previous cultivation efforts have suggested an important role of trace elements for the proliferation of Acidobacteria, however, the impact of these metals on their growth and metabolism is not known. In order to gain insight into this relationship, we evaluated the effect of trace element solution SL10 on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to the genus Granulicella and studied the proteomic responses to manganese (Mn). Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal salts. Variations in tolerance to metal salt concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. Furthermore, Granulicella sp. 5B5 might be more adapted to survive in an environment with higher concentration of several metal ions when compared to Granulicella sp. WH15. The proteomic profiles of both strains indicated that Mn was more important in enhancing enzymatic activity than to protein expression regulation. In the genomic analyses, we did not find the most common transcriptional regulation of Mn homeostasis, but we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment from which these two Granulicella strains were isolated.
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spelling doaj.art-23084a76c41c4792aa8c10568d91c07f2022-12-21T20:37:34ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01227538660Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”Ohana Y. A. Costa0Ohana Y. A. Costa1Chidinma Oguejiofor2Daniela Zühlke3Cristine C. Barreto4Christine Wünsche5Katharina Riedel6Eiko E. Kuramae7Eiko E. Kuramae8Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, NetherlandsInstitute of Biology Leiden, Leiden University, Leiden, NetherlandsDepartment of Soil Science and Meteorology, Michael Okpara University of Agriculture, Umudike, NigeriaInstitute of Microbiology, University of Greifswald, Greifswald, GermanyGenomic Sciences and Biotechnology Program, Catholic University of Brasilia, Distrito Federal, BrazilInstitute of Microbiology, University of Greifswald, Greifswald, GermanyInstitute of Microbiology, University of Greifswald, Greifswald, GermanyDepartment of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, NetherlandsEcology and Biodiversity, Institute of Environmental Biology, Utrecht University, Utrecht, NetherlandsAcidobacteria represents one of the most dominant bacterial groups across diverse ecosystems. However, insight into their ecology and physiology has been hampered by difficulties in cultivating members of this phylum. Previous cultivation efforts have suggested an important role of trace elements for the proliferation of Acidobacteria, however, the impact of these metals on their growth and metabolism is not known. In order to gain insight into this relationship, we evaluated the effect of trace element solution SL10 on the growth of two strains (5B5 and WH15) of Acidobacteria belonging to the genus Granulicella and studied the proteomic responses to manganese (Mn). Granulicella species had highest growth with the addition of Mn, as well as higher tolerance to this metal compared to seven other metal salts. Variations in tolerance to metal salt concentrations suggests that Granulicella sp. strains possess different mechanisms to deal with metal ion homeostasis and stress. Furthermore, Granulicella sp. 5B5 might be more adapted to survive in an environment with higher concentration of several metal ions when compared to Granulicella sp. WH15. The proteomic profiles of both strains indicated that Mn was more important in enhancing enzymatic activity than to protein expression regulation. In the genomic analyses, we did not find the most common transcriptional regulation of Mn homeostasis, but we found candidate transporters that could be potentially involved in Mn homeostasis for Granulicella species. The presence of such transporters might be involved in tolerance to higher Mn concentrations, improving the adaptability of bacteria to metal enriched environments, such as the decaying wood-rich Mn environment from which these two Granulicella strains were isolated.https://www.frontiersin.org/article/10.3389/fmicb.2020.01227/fullAcidobacteriaGranulicellagenomeproteomemanganesemetabolism
spellingShingle Ohana Y. A. Costa
Ohana Y. A. Costa
Chidinma Oguejiofor
Daniela Zühlke
Cristine C. Barreto
Christine Wünsche
Katharina Riedel
Eiko E. Kuramae
Eiko E. Kuramae
Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
Frontiers in Microbiology
Acidobacteria
Granulicella
genome
proteome
manganese
metabolism
title Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
title_full Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
title_fullStr Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
title_full_unstemmed Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
title_short Impact of Different Trace Elements on the Growth and Proteome of Two Strains of Granulicella, Class “Acidobacteriia”
title_sort impact of different trace elements on the growth and proteome of two strains of granulicella class acidobacteriia
topic Acidobacteria
Granulicella
genome
proteome
manganese
metabolism
url https://www.frontiersin.org/article/10.3389/fmicb.2020.01227/full
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