Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus
Fatma I Albeladi,1 Mostafa M Mostafa,2,3 Mohamed A Zayed,4,5 Hazem Atta2,3 1Department of Nephrology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; 2Department of Clinical Biochemistry, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia; 3Departmen...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-07-01
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| Series: | International Journal of General Medicine |
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| Online Access: | https://www.dovepress.com/association-of-polymorphisms-in-antioxidant-enzyme-encoding-genes-with-peer-reviewed-fulltext-article-IJGM |
| _version_ | 1828335212891734016 |
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| author | Albeladi FI Mostafa MM Zayed MA Atta H |
| author_facet | Albeladi FI Mostafa MM Zayed MA Atta H |
| author_sort | Albeladi FI |
| collection | DOAJ |
| description | Fatma I Albeladi,1 Mostafa M Mostafa,2,3 Mohamed A Zayed,4,5 Hazem Atta2,3 1Department of Nephrology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; 2Department of Clinical Biochemistry, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia; 3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt; 4Department of Medical Physiology, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia; 5Department of Medical Physiology, Faculty of Medicine, Menoufia University, Menoufia, EgyptCorrespondence: Mohamed A Zayed, Department of Medical Physiology, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, 21589, Saudi Arabia, Tel +966562603213, Fax +9666400000, Email mazayed@kau.edu.saIntroduction: An imbalance between reactive oxygen species (ROS) generation and the defence mechanisms underlying the activity of antioxidant enzymes has been demonstrated as the leading pathology in diabetes mellitus (DM)-related microvascular complications.Purpose: This study aims to evaluate the association between polymorphisms in antioxidant enzyme-encoding genes: catalase (CAT); manganese superoxide dismutase (Mn-SOD); glutathione S transferase M1 (GSTM1); and GSTT1 glutathione S transferase T1 (GSTT1), and the risk of type II diabetic nephropathy (DN) in the Saudi population.Patients and Methods: The present study involved 64 type II DM patients with nephropathy and 64 type II diabetes patients without nephropathy from the King Abdulaziz University (KAU) Hospital. They underwent real-time PCR genotyping for the Mn-SOD and CAT genes. Multiplex PCR was used to detect GSTM1- and GSTT1-null polymorphisms.Results: A statistically significant difference was observed between the case and control groups with regard to polymorphisms in the CAT gene (P = 0.037), but not for polymorphisms in the Mn-SOD (P = 0.64) gene. In addition, a statistically significant association was observed between null polymorphisms of the GSTT1 and GSTM1 genes and DN in the case and control groups (P = 0.046 and P = 0.035, respectively).Conclusion: Our results showed that the genetic ability to combat oxidative stress may play a major role in DN pathogenesis in Saudi type II DM patients. These polymorphisms in antioxidant enzyme-encoding genes could be used as independent genetic markers for the construction of risk prediction models for kidney-related complications in type II DM patients.Keywords: diabetic nephropathy, gene polymorphism, oxidative stress |
| first_indexed | 2024-04-13T21:43:11Z |
| format | Article |
| id | doaj.art-23091ec8bdd04b19a375175047884e99 |
| institution | Directory Open Access Journal |
| issn | 1178-7074 |
| language | English |
| last_indexed | 2024-04-13T21:43:11Z |
| publishDate | 2022-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of General Medicine |
| spelling | doaj.art-23091ec8bdd04b19a375175047884e992022-12-22T02:28:40ZengDove Medical PressInternational Journal of General Medicine1178-70742022-07-01Volume 155919592876303Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes MellitusAlbeladi FIMostafa MMZayed MAAtta HFatma I Albeladi,1 Mostafa M Mostafa,2,3 Mohamed A Zayed,4,5 Hazem Atta2,3 1Department of Nephrology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia; 2Department of Clinical Biochemistry, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia; 3Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt; 4Department of Medical Physiology, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, Saudi Arabia; 5Department of Medical Physiology, Faculty of Medicine, Menoufia University, Menoufia, EgyptCorrespondence: Mohamed A Zayed, Department of Medical Physiology, Faculty of Medicine in Rabigh, King Abdulaziz University, Jeddah, 21589, Saudi Arabia, Tel +966562603213, Fax +9666400000, Email mazayed@kau.edu.saIntroduction: An imbalance between reactive oxygen species (ROS) generation and the defence mechanisms underlying the activity of antioxidant enzymes has been demonstrated as the leading pathology in diabetes mellitus (DM)-related microvascular complications.Purpose: This study aims to evaluate the association between polymorphisms in antioxidant enzyme-encoding genes: catalase (CAT); manganese superoxide dismutase (Mn-SOD); glutathione S transferase M1 (GSTM1); and GSTT1 glutathione S transferase T1 (GSTT1), and the risk of type II diabetic nephropathy (DN) in the Saudi population.Patients and Methods: The present study involved 64 type II DM patients with nephropathy and 64 type II diabetes patients without nephropathy from the King Abdulaziz University (KAU) Hospital. They underwent real-time PCR genotyping for the Mn-SOD and CAT genes. Multiplex PCR was used to detect GSTM1- and GSTT1-null polymorphisms.Results: A statistically significant difference was observed between the case and control groups with regard to polymorphisms in the CAT gene (P = 0.037), but not for polymorphisms in the Mn-SOD (P = 0.64) gene. In addition, a statistically significant association was observed between null polymorphisms of the GSTT1 and GSTM1 genes and DN in the case and control groups (P = 0.046 and P = 0.035, respectively).Conclusion: Our results showed that the genetic ability to combat oxidative stress may play a major role in DN pathogenesis in Saudi type II DM patients. These polymorphisms in antioxidant enzyme-encoding genes could be used as independent genetic markers for the construction of risk prediction models for kidney-related complications in type II DM patients.Keywords: diabetic nephropathy, gene polymorphism, oxidative stresshttps://www.dovepress.com/association-of-polymorphisms-in-antioxidant-enzyme-encoding-genes-with-peer-reviewed-fulltext-article-IJGMdiabetic nephropathygene polymorphismoxidative stress. |
| spellingShingle | Albeladi FI Mostafa MM Zayed MA Atta H Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus International Journal of General Medicine diabetic nephropathy gene polymorphism oxidative stress. |
| title | Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus |
| title_full | Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus |
| title_fullStr | Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus |
| title_full_unstemmed | Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus |
| title_short | Association of Polymorphisms in Antioxidant Enzyme-Encoding Genes with Diabetic Nephropathy in a Group of Saudi Arabian Patients with Type II Diabetes Mellitus |
| title_sort | association of polymorphisms in antioxidant enzyme encoding genes with diabetic nephropathy in a group of saudi arabian patients with type ii diabetes mellitus |
| topic | diabetic nephropathy gene polymorphism oxidative stress. |
| url | https://www.dovepress.com/association-of-polymorphisms-in-antioxidant-enzyme-encoding-genes-with-peer-reviewed-fulltext-article-IJGM |
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