H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production
Hairy roots (HRs) grown in vitro are a powerful platform for plant biotechnological advances and for the bio-based production of metabolites of interest. In this work, black carrot HRs able to accumulate anthocyanin as major secondary metabolite were used. Biomass and anthocyanin accumulation were i...
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MDPI AG
2021-12-01
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| Series: | Plants |
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| author | Gregorio Barba-Espín Christian Martínez-Jiménez Alberto Izquierdo-Martínez José R. Acosta-Motos José A. Hernández Pedro Díaz-Vivancos |
| author_facet | Gregorio Barba-Espín Christian Martínez-Jiménez Alberto Izquierdo-Martínez José R. Acosta-Motos José A. Hernández Pedro Díaz-Vivancos |
| author_sort | Gregorio Barba-Espín |
| collection | DOAJ |
| description | Hairy roots (HRs) grown in vitro are a powerful platform for plant biotechnological advances and for the bio-based production of metabolites of interest. In this work, black carrot HRs able to accumulate anthocyanin as major secondary metabolite were used. Biomass and anthocyanin accumulation were improved by modulating growth medium composition—different Murashige & Skoog (MS)-based media—and H<sub>2</sub>O<sub>2</sub>-elicitation, and the level of the main antioxidant enzymes on elicited HRs was measured. Higher growth was obtained on liquid 1/2 MS medium supplemented with 60 g/L sucrose for HRs grown over 20 days. In this medium, 200 µM H<sub>2</sub>O<sub>2</sub> applied on day 12 induced anthocyanin accumulation by 20%. The activity of superoxide dismutase (SOD)—which generates H<sub>2</sub>O<sub>2</sub> from O<sub>2</sub><sup>•−</sup>—increased by over 50%, whereas the activity of H<sub>2</sub>O<sub>2</sub>-scavenging enzymes was not enhanced. Elicitation in the HRs can result in a controlled oxidative burst, in which SOD activity increased H<sub>2</sub>O<sub>2</sub> levels, whereas anthocyanins, as effective reactive oxygen species scavengers, could be induced to modulate the oxidative burst generated. Moreover, given the proven stability of the HR lines used and their remarkable productivity, this system appears as suitable for elucidating the interplay between antioxidant and secondary metabolism. |
| first_indexed | 2024-03-10T03:16:16Z |
| format | Article |
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| language | English |
| last_indexed | 2024-03-10T03:16:16Z |
| publishDate | 2021-12-01 |
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| series | Plants |
| spelling | doaj.art-230a1759336d46fc8cf0fd683d3a72482023-11-23T10:12:40ZengMDPI AGPlants2223-77472021-12-011012275310.3390/plants10122753H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin ProductionGregorio Barba-Espín0Christian Martínez-Jiménez1Alberto Izquierdo-Martínez2José R. Acosta-Motos3José A. Hernández4Pedro Díaz-Vivancos5Department of Fruit Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Murcia, SpainDepartment of Fruit Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Murcia, SpainDepartment of Fruit Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Murcia, SpainDepartment of Fruit Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Murcia, SpainDepartment of Fruit Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Murcia, SpainDepartment of Fruit Breeding, CEBAS-CSIC, Campus Universitario de Espinardo, 30100 Murcia, SpainHairy roots (HRs) grown in vitro are a powerful platform for plant biotechnological advances and for the bio-based production of metabolites of interest. In this work, black carrot HRs able to accumulate anthocyanin as major secondary metabolite were used. Biomass and anthocyanin accumulation were improved by modulating growth medium composition—different Murashige & Skoog (MS)-based media—and H<sub>2</sub>O<sub>2</sub>-elicitation, and the level of the main antioxidant enzymes on elicited HRs was measured. Higher growth was obtained on liquid 1/2 MS medium supplemented with 60 g/L sucrose for HRs grown over 20 days. In this medium, 200 µM H<sub>2</sub>O<sub>2</sub> applied on day 12 induced anthocyanin accumulation by 20%. The activity of superoxide dismutase (SOD)—which generates H<sub>2</sub>O<sub>2</sub> from O<sub>2</sub><sup>•−</sup>—increased by over 50%, whereas the activity of H<sub>2</sub>O<sub>2</sub>-scavenging enzymes was not enhanced. Elicitation in the HRs can result in a controlled oxidative burst, in which SOD activity increased H<sub>2</sub>O<sub>2</sub> levels, whereas anthocyanins, as effective reactive oxygen species scavengers, could be induced to modulate the oxidative burst generated. Moreover, given the proven stability of the HR lines used and their remarkable productivity, this system appears as suitable for elucidating the interplay between antioxidant and secondary metabolism.https://www.mdpi.com/2223-7747/10/12/2753antioxidant metabolismelicitationhydrogen peroxidein vitro culturesuperoxide dismutasereactive oxygen species (ROS) scavenging |
| spellingShingle | Gregorio Barba-Espín Christian Martínez-Jiménez Alberto Izquierdo-Martínez José R. Acosta-Motos José A. Hernández Pedro Díaz-Vivancos H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production Plants antioxidant metabolism elicitation hydrogen peroxide in vitro culture superoxide dismutase reactive oxygen species (ROS) scavenging |
| title | H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production |
| title_full | H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production |
| title_fullStr | H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production |
| title_full_unstemmed | H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production |
| title_short | H<sub>2</sub>O<sub>2</sub>-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production |
| title_sort | h sub 2 sub o sub 2 sub elicitation of black carrot hairy roots induces a controlled oxidative burst leading to increased anthocyanin production |
| topic | antioxidant metabolism elicitation hydrogen peroxide in vitro culture superoxide dismutase reactive oxygen species (ROS) scavenging |
| url | https://www.mdpi.com/2223-7747/10/12/2753 |
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