Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential
<i>Polygonum chinense</i> Linn. is a medicinal and invasive plant that belongs to the family Polygonaceae. The pharmacological activities and phytochemical constituents of <i>Polygonum chinense</i> are well reported, but the allelopathic effects and potent allelopathic substa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/7/1577 |
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| author | Thang Lam Lun Arihiro Iwasaki Kiyotake Suenaga Hisashi Kato-Noguchi |
| author_facet | Thang Lam Lun Arihiro Iwasaki Kiyotake Suenaga Hisashi Kato-Noguchi |
| author_sort | Thang Lam Lun |
| collection | DOAJ |
| description | <i>Polygonum chinense</i> Linn. is a medicinal and invasive plant that belongs to the family Polygonaceae. The pharmacological activities and phytochemical constituents of <i>Polygonum chinense</i> are well reported, but the allelopathic effects and potent allelopathic substances of <i>P. chinense</i> remain to be investigated. Hence, this experiment was conducted to separate and characterize potentially allelopathic substances from an extract of the <i>Polygonum chinense</i> plant. The <i>Polygonum chinense</i> plant extracts highly suppressed the growth of cress (<i>Lepidium sativum</i> L.), lettuce (<i>Lactuca sativa</i> L.), barnyard grass (<i>Echinochloa crusgalli</i> (L.) P. Beauv.), and timothy grass (<i>Phleum pratense</i> L.) seedlings in a species- and concentration-dependent way. Two active substances were separated using a series of purification procedures and determined through spectral analysis as (−)-3-hydroxy-<i>β</i>-ionone and (−)-3-hydroxy-7,8-dihydro-<i>β</i>-ionone. These two compounds significantly suppressed the seedling growth of <i>Lepidium sativum</i> (cress) at concentrations of 0.01 and 1 mM, respectively. The extract concentrations necessary for 50% growth inhibition (<i>I</i><sub>50</sub> values) of the cress hypocotyls and roots were 0.05 and 0.07 mM for (−)-3-hydroxy-<i>β</i>-ionone, respectively, and 0.42 and 1.29 mM for (−)-3-hydroxy-7,8-<i>β</i>-ionone, respectively. These findings suggest that these two compounds are in charge of the inhibitory effects of the <i>Polygonum chinense</i> extract and may serve as weed control agents. |
| first_indexed | 2024-03-11T05:26:29Z |
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| language | English |
| last_indexed | 2024-03-11T05:26:29Z |
| publishDate | 2023-04-01 |
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| series | Plants |
| spelling | doaj.art-230bd27d916f4fe09db289e1273031232023-11-17T17:24:35ZengMDPI AGPlants2223-77472023-04-01127157710.3390/plants12071577Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal PotentialThang Lam Lun0Arihiro Iwasaki1Kiyotake Suenaga2Hisashi Kato-Noguchi3Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki 761-0795, Kagawa, JapanDepartment of Chemistry, Faculty of Science and Technology, Keio University, Kohoku, Yokohama 223-8522, Kanagawa, JapanDepartment of Chemistry, Faculty of Science and Technology, Keio University, Kohoku, Yokohama 223-8522, Kanagawa, JapanDepartment of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki 761-0795, Kagawa, Japan<i>Polygonum chinense</i> Linn. is a medicinal and invasive plant that belongs to the family Polygonaceae. The pharmacological activities and phytochemical constituents of <i>Polygonum chinense</i> are well reported, but the allelopathic effects and potent allelopathic substances of <i>P. chinense</i> remain to be investigated. Hence, this experiment was conducted to separate and characterize potentially allelopathic substances from an extract of the <i>Polygonum chinense</i> plant. The <i>Polygonum chinense</i> plant extracts highly suppressed the growth of cress (<i>Lepidium sativum</i> L.), lettuce (<i>Lactuca sativa</i> L.), barnyard grass (<i>Echinochloa crusgalli</i> (L.) P. Beauv.), and timothy grass (<i>Phleum pratense</i> L.) seedlings in a species- and concentration-dependent way. Two active substances were separated using a series of purification procedures and determined through spectral analysis as (−)-3-hydroxy-<i>β</i>-ionone and (−)-3-hydroxy-7,8-dihydro-<i>β</i>-ionone. These two compounds significantly suppressed the seedling growth of <i>Lepidium sativum</i> (cress) at concentrations of 0.01 and 1 mM, respectively. The extract concentrations necessary for 50% growth inhibition (<i>I</i><sub>50</sub> values) of the cress hypocotyls and roots were 0.05 and 0.07 mM for (−)-3-hydroxy-<i>β</i>-ionone, respectively, and 0.42 and 1.29 mM for (−)-3-hydroxy-7,8-<i>β</i>-ionone, respectively. These findings suggest that these two compounds are in charge of the inhibitory effects of the <i>Polygonum chinense</i> extract and may serve as weed control agents.https://www.mdpi.com/2223-7747/12/7/1577<i>Polygonum chinense</i>allelopathic substances(−)-3-hydroxy-<i>β</i>-ionone(−)-3-hydroxy-7,8-<i>β</i>-ionone |
| spellingShingle | Thang Lam Lun Arihiro Iwasaki Kiyotake Suenaga Hisashi Kato-Noguchi Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential Plants <i>Polygonum chinense</i> allelopathic substances (−)-3-hydroxy-<i>β</i>-ionone (−)-3-hydroxy-7,8-<i>β</i>-ionone |
| title | Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential |
| title_full | Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential |
| title_fullStr | Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential |
| title_full_unstemmed | Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential |
| title_short | Isolation and Identification of Plant-Growth Inhibitory Constituents from <i>Polygonum chinense</i> Linn and Evaluation of Their Bioherbicidal Potential |
| title_sort | isolation and identification of plant growth inhibitory constituents from i polygonum chinense i linn and evaluation of their bioherbicidal potential |
| topic | <i>Polygonum chinense</i> allelopathic substances (−)-3-hydroxy-<i>β</i>-ionone (−)-3-hydroxy-7,8-<i>β</i>-ionone |
| url | https://www.mdpi.com/2223-7747/12/7/1577 |
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