Plasma membrane aquaporins mediates vesicle stability in broccoli.
The use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membr...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5805300?pdf=render |
| _version_ | 1818036110907932672 |
|---|---|
| author | Maria Del Carmen Martínez-Ballesta Pablo García-Gomez Lucía Yepes-Molina Angel L Guarnizo José A Teruel Micaela Carvajal |
| author_facet | Maria Del Carmen Martínez-Ballesta Pablo García-Gomez Lucía Yepes-Molina Angel L Guarnizo José A Teruel Micaela Carvajal |
| author_sort | Maria Del Carmen Martínez-Ballesta |
| collection | DOAJ |
| description | The use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membrane osmotic water permeability and the stability of protein secondary structure. Here, we describe for first time an increase in plant aquaporins acetylation under high salinity. Higher osmotic water permeability in NaCl vesicles has been related to higher acetylation, upregulation of aquaporins, and a more stable environment to thermal denaturation. Based on our findings, we propose that aquaporins play an important role in vesicle stability. |
| first_indexed | 2024-12-10T07:05:45Z |
| format | Article |
| id | doaj.art-230d0db0f6ea433283808404a29114e3 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:05:45Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-230d0db0f6ea433283808404a29114e32022-12-22T01:58:11ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01132e019242210.1371/journal.pone.0192422Plasma membrane aquaporins mediates vesicle stability in broccoli.Maria Del Carmen Martínez-BallestaPablo García-GomezLucía Yepes-MolinaAngel L GuarnizoJosé A TeruelMicaela CarvajalThe use of in vitro membrane vesicles is attractive because of possible applications in therapies. Here we aimed to compare the stability and functionality of plasma membrane vesicles extracted from control and salt-treated broccoli. The impact of the amount of aquaporins was related to plasma membrane osmotic water permeability and the stability of protein secondary structure. Here, we describe for first time an increase in plant aquaporins acetylation under high salinity. Higher osmotic water permeability in NaCl vesicles has been related to higher acetylation, upregulation of aquaporins, and a more stable environment to thermal denaturation. Based on our findings, we propose that aquaporins play an important role in vesicle stability.http://europepmc.org/articles/PMC5805300?pdf=render |
| spellingShingle | Maria Del Carmen Martínez-Ballesta Pablo García-Gomez Lucía Yepes-Molina Angel L Guarnizo José A Teruel Micaela Carvajal Plasma membrane aquaporins mediates vesicle stability in broccoli. PLoS ONE |
| title | Plasma membrane aquaporins mediates vesicle stability in broccoli. |
| title_full | Plasma membrane aquaporins mediates vesicle stability in broccoli. |
| title_fullStr | Plasma membrane aquaporins mediates vesicle stability in broccoli. |
| title_full_unstemmed | Plasma membrane aquaporins mediates vesicle stability in broccoli. |
| title_short | Plasma membrane aquaporins mediates vesicle stability in broccoli. |
| title_sort | plasma membrane aquaporins mediates vesicle stability in broccoli |
| url | http://europepmc.org/articles/PMC5805300?pdf=render |
| work_keys_str_mv | AT mariadelcarmenmartinezballesta plasmamembraneaquaporinsmediatesvesiclestabilityinbroccoli AT pablogarciagomez plasmamembraneaquaporinsmediatesvesiclestabilityinbroccoli AT luciayepesmolina plasmamembraneaquaporinsmediatesvesiclestabilityinbroccoli AT angellguarnizo plasmamembraneaquaporinsmediatesvesiclestabilityinbroccoli AT joseateruel plasmamembraneaquaporinsmediatesvesiclestabilityinbroccoli AT micaelacarvajal plasmamembraneaquaporinsmediatesvesiclestabilityinbroccoli |