Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it>
<p>Abstract</p> <p>Background</p> <p>In the context of the Sterile Insect Technique (SIT), radiation-induced sterility in the malaria mosquito <it>Anopheles arabiensis </it>Patton (Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays i...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2006-05-01
|
| Series: | Malaria Journal |
| Online Access: | http://www.malariajournal.com/content/5/1/41 |
| _version_ | 1818807901362323456 |
|---|---|
| author | Knols Bart GJ Parker Andrew G Helinski Michelle EH |
| author_facet | Knols Bart GJ Parker Andrew G Helinski Michelle EH |
| author_sort | Knols Bart GJ |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>In the context of the Sterile Insect Technique (SIT), radiation-induced sterility in the malaria mosquito <it>Anopheles arabiensis </it>Patton (Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays in the pupal or adult stage and dose-sterility curves were determined.</p> <p>Methods</p> <p>Pupae were irradiated shortly before emergence (at 22–26 hrs of age), and adults <24 hrs post emergence. Doses tested ranged between 0 and 100 Gy. The effects of irradiation on adult emergence, male survival, induced sterility and insemination capability were evaluated. Emergence and insemination data were analysed using independent t-tests against the control. Correlation analyses were performed for insemination rate and dose and insemination and fecundity. Male survival was analysed using Kaplan-Meier survival analyses. Finally, the calculated residual fertility values were inverse-normal transformed and linear regression analyses performed.</p> <p>Results</p> <p>Irradiation of pupae, for all doses tested, had no effect on adult emergence. Survival curves of males irradiated as pupae or adults were similar or even slightly higher than non-irradiated males. Overall, adults appeared to be slightly more susceptible to irradiation, although no significant differences for individual doses were observed. In the pupal stage, a significant negative correlation was found between insemination and dose, but the correlation-coefficient was associated with less than 25% of the total variation. A review of the literature indicated that <it>An. arabiensis </it>is more radiation resistant than other anopheline mosquitoes.</p> <p>Conclusion</p> <p>The optimal dose for male insects to be released in an SIT programme depends on their level of sterility and competitiveness. The use of semi-sterilizing doses to produce more competitive insects is discussed. The most convenient developmental stage for mosquito irradiation on a mass-scale are pupae, but pupal irradiation resulted in a lower insemination rate at the highest dose compared to adult irradiation. On the basis of this study, a suitable dose range that includes semi-sterilizing doses is identified to initiate competitiveness experiments for males irradiated at both developmental stages.</p> |
| first_indexed | 2024-12-18T19:33:01Z |
| format | Article |
| id | doaj.art-230dd41bab5446edb66627a7265ab621 |
| institution | Directory Open Access Journal |
| issn | 1475-2875 |
| language | English |
| last_indexed | 2024-12-18T19:33:01Z |
| publishDate | 2006-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Malaria Journal |
| spelling | doaj.art-230dd41bab5446edb66627a7265ab6212022-12-21T20:55:41ZengBMCMalaria Journal1475-28752006-05-01514110.1186/1475-2875-5-41Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it>Knols Bart GJParker Andrew GHelinski Michelle EH<p>Abstract</p> <p>Background</p> <p>In the context of the Sterile Insect Technique (SIT), radiation-induced sterility in the malaria mosquito <it>Anopheles arabiensis </it>Patton (Diptera: Culicidae) was studied. Male mosquitoes were exposed to gamma rays in the pupal or adult stage and dose-sterility curves were determined.</p> <p>Methods</p> <p>Pupae were irradiated shortly before emergence (at 22–26 hrs of age), and adults <24 hrs post emergence. Doses tested ranged between 0 and 100 Gy. The effects of irradiation on adult emergence, male survival, induced sterility and insemination capability were evaluated. Emergence and insemination data were analysed using independent t-tests against the control. Correlation analyses were performed for insemination rate and dose and insemination and fecundity. Male survival was analysed using Kaplan-Meier survival analyses. Finally, the calculated residual fertility values were inverse-normal transformed and linear regression analyses performed.</p> <p>Results</p> <p>Irradiation of pupae, for all doses tested, had no effect on adult emergence. Survival curves of males irradiated as pupae or adults were similar or even slightly higher than non-irradiated males. Overall, adults appeared to be slightly more susceptible to irradiation, although no significant differences for individual doses were observed. In the pupal stage, a significant negative correlation was found between insemination and dose, but the correlation-coefficient was associated with less than 25% of the total variation. A review of the literature indicated that <it>An. arabiensis </it>is more radiation resistant than other anopheline mosquitoes.</p> <p>Conclusion</p> <p>The optimal dose for male insects to be released in an SIT programme depends on their level of sterility and competitiveness. The use of semi-sterilizing doses to produce more competitive insects is discussed. The most convenient developmental stage for mosquito irradiation on a mass-scale are pupae, but pupal irradiation resulted in a lower insemination rate at the highest dose compared to adult irradiation. On the basis of this study, a suitable dose range that includes semi-sterilizing doses is identified to initiate competitiveness experiments for males irradiated at both developmental stages.</p>http://www.malariajournal.com/content/5/1/41 |
| spellingShingle | Knols Bart GJ Parker Andrew G Helinski Michelle EH Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it> Malaria Journal |
| title | Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it> |
| title_full | Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it> |
| title_fullStr | Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it> |
| title_full_unstemmed | Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it> |
| title_short | Radiation-induced sterility for pupal and adult stages of the malaria mosquito <it>Anopheles arabiensis</it> |
| title_sort | radiation induced sterility for pupal and adult stages of the malaria mosquito it anopheles arabiensis it |
| url | http://www.malariajournal.com/content/5/1/41 |
| work_keys_str_mv | AT knolsbartgj radiationinducedsterilityforpupalandadultstagesofthemalariamosquitoitanophelesarabiensisit AT parkerandrewg radiationinducedsterilityforpupalandadultstagesofthemalariamosquitoitanophelesarabiensisit AT helinskimichelleeh radiationinducedsterilityforpupalandadultstagesofthemalariamosquitoitanophelesarabiensisit |