Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females
Pheromones are biologically important in fruit fly mating systems, and also have potential applications as attractants or mating disrupters for pest management. <i>Bactrocera kraussi</i> (Hardy) (Diptera: Tephritidae) is a polyphagous pest fruit fly for which the chemical profile of rect...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/16/5024 |
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| author | Sally Noushini Soo Jean Park Jeanneth Perez Danielle Holgate Vivian Mendez Ian M. Jamie Joanne F. Jamie Phillip W. Taylor |
| author_facet | Sally Noushini Soo Jean Park Jeanneth Perez Danielle Holgate Vivian Mendez Ian M. Jamie Joanne F. Jamie Phillip W. Taylor |
| author_sort | Sally Noushini |
| collection | DOAJ |
| description | Pheromones are biologically important in fruit fly mating systems, and also have potential applications as attractants or mating disrupters for pest management. <i>Bactrocera kraussi</i> (Hardy) (Diptera: Tephritidae) is a polyphagous pest fruit fly for which the chemical profile of rectal glands is available for males but not for females. There have been no studies of the volatile emissions of either sex or of electrophysiological responses to these compounds. The present study (i) establishes the chemical profiles of rectal gland contents and volatiles emitted by both sexes of <i>B. kraussi</i> by gas chromatography–mass spectrometry (GC–MS) and (ii) evaluates the detection of the identified compounds by gas chromatography–electroantennogram detection (GC–EAD) and –electropalpogram detection (GC–EPD). Sixteen compounds are identified in the rectal glands of male <i>B. kraussi</i> and 29 compounds are identified in the rectal glands of females. Of these compounds, 5 were detected in the headspace of males and 13 were detected in the headspace of females. GC–EPD assays recorded strong signals in both sexes against (<i>E</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, 2-ethyl-7-mehtyl-1,6-dioxaspiro[4.5]decane isomer 2, (<i>E</i>,<i>Z</i>)/(<i>Z</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, and (<i>Z</i>,<i>Z</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane. Male antennae responded to (<i>E</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, 2-methyl-6-pentyl-3,4-dihydro-2<i>H</i>-pyran, 6-hexyl-2-methyl-3,4-dihydro-2<i>H</i>-pyran, 6-oxononan-1-ol, ethyl dodecanoate, ethyl tetradecanoate and ethyl (<i>Z</i>)-hexadec-9-enoate, whereas female antennae responded to (<i>E</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane and 2-methyl-6-pentyl-3,4-dihydro-2<i>H</i>-pyran only. These compounds are candidates as pheromones mediating sexual interactions in <i>B. kraussi</i>. |
| first_indexed | 2024-03-10T08:31:56Z |
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| spelling | doaj.art-230eed7cbfae48f4bfa4f18177aad3312023-11-22T08:55:30ZengMDPI AGMolecules1420-30492021-08-012616502410.3390/molecules26165024Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and FemalesSally Noushini0Soo Jean Park1Jeanneth Perez2Danielle Holgate3Vivian Mendez4Ian M. Jamie5Joanne F. Jamie6Phillip W. Taylor7Department of Molecular Sciences, Macquarie University, Sydney, NSW 2109, AustraliaAustralian Research Council Industrial Transformation Training Centre for Fruit Fly Biosecurity Innovation, Macquarie University, Sydney, NSW 2109, AustraliaAustralian Research Council Industrial Transformation Training Centre for Fruit Fly Biosecurity Innovation, Macquarie University, Sydney, NSW 2109, AustraliaDepartment of Molecular Sciences, Macquarie University, Sydney, NSW 2109, AustraliaAustralian Research Council Industrial Transformation Training Centre for Fruit Fly Biosecurity Innovation, Macquarie University, Sydney, NSW 2109, AustraliaDepartment of Molecular Sciences, Macquarie University, Sydney, NSW 2109, AustraliaDepartment of Molecular Sciences, Macquarie University, Sydney, NSW 2109, AustraliaAustralian Research Council Industrial Transformation Training Centre for Fruit Fly Biosecurity Innovation, Macquarie University, Sydney, NSW 2109, AustraliaPheromones are biologically important in fruit fly mating systems, and also have potential applications as attractants or mating disrupters for pest management. <i>Bactrocera kraussi</i> (Hardy) (Diptera: Tephritidae) is a polyphagous pest fruit fly for which the chemical profile of rectal glands is available for males but not for females. There have been no studies of the volatile emissions of either sex or of electrophysiological responses to these compounds. The present study (i) establishes the chemical profiles of rectal gland contents and volatiles emitted by both sexes of <i>B. kraussi</i> by gas chromatography–mass spectrometry (GC–MS) and (ii) evaluates the detection of the identified compounds by gas chromatography–electroantennogram detection (GC–EAD) and –electropalpogram detection (GC–EPD). Sixteen compounds are identified in the rectal glands of male <i>B. kraussi</i> and 29 compounds are identified in the rectal glands of females. Of these compounds, 5 were detected in the headspace of males and 13 were detected in the headspace of females. GC–EPD assays recorded strong signals in both sexes against (<i>E</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, 2-ethyl-7-mehtyl-1,6-dioxaspiro[4.5]decane isomer 2, (<i>E</i>,<i>Z</i>)/(<i>Z</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, and (<i>Z</i>,<i>Z</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane. Male antennae responded to (<i>E</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane, 2-methyl-6-pentyl-3,4-dihydro-2<i>H</i>-pyran, 6-hexyl-2-methyl-3,4-dihydro-2<i>H</i>-pyran, 6-oxononan-1-ol, ethyl dodecanoate, ethyl tetradecanoate and ethyl (<i>Z</i>)-hexadec-9-enoate, whereas female antennae responded to (<i>E</i>,<i>E</i>)-2,8-dimethyl-1,7-dioxaspiro[5.5]undecane and 2-methyl-6-pentyl-3,4-dihydro-2<i>H</i>-pyran only. These compounds are candidates as pheromones mediating sexual interactions in <i>B. kraussi</i>.https://www.mdpi.com/1420-3049/26/16/5024TephritidaepheromoneolfactionelectrophysiologyGC–MSEAD |
| spellingShingle | Sally Noushini Soo Jean Park Jeanneth Perez Danielle Holgate Vivian Mendez Ian M. Jamie Joanne F. Jamie Phillip W. Taylor Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females Molecules Tephritidae pheromone olfaction electrophysiology GC–MS EAD |
| title | Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females |
| title_full | Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females |
| title_fullStr | Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females |
| title_full_unstemmed | Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females |
| title_short | Electrophysiological Responses of <i>Bactrocera kraussi</i> (Hardy) (Tephritidae) to Rectal Gland Secretions and Headspace Volatiles Emitted by Conspecific Males and Females |
| title_sort | electrophysiological responses of i bactrocera kraussi i hardy tephritidae to rectal gland secretions and headspace volatiles emitted by conspecific males and females |
| topic | Tephritidae pheromone olfaction electrophysiology GC–MS EAD |
| url | https://www.mdpi.com/1420-3049/26/16/5024 |
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