LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation
Abstract Background Our preliminary RNA-Seq data revealed altered expression of small nucleolar RNA host gene 9 (SNHG9) in osteoarthritis (OA) and its reverse correlation with miR-34a, which can regulate chondrocyte apoptosis in rat OA model. This study was therefore carried out to investigate the p...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-08-01
|
| Series: | BMC Musculoskeletal Disorders |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12891-020-03497-7 |
| _version_ | 1818331835315257344 |
|---|---|
| author | Hongfei Zhang Jinglian Li Weiguang Shao Naipeng Shen |
| author_facet | Hongfei Zhang Jinglian Li Weiguang Shao Naipeng Shen |
| author_sort | Hongfei Zhang |
| collection | DOAJ |
| description | Abstract Background Our preliminary RNA-Seq data revealed altered expression of small nucleolar RNA host gene 9 (SNHG9) in osteoarthritis (OA) and its reverse correlation with miR-34a, which can regulate chondrocyte apoptosis in rat OA model. This study was therefore carried out to investigate the potential interaction between SNHG9 and miR-34a in OA. Methods A total of 60 healthy volunteers (Control group) as well as 60 OA patients (OA group) were enrolled in this study. Transfections, RT-qPCR, methylation-specific PCR (MSP) and cell apoptosis assay were performed. Results We found that SNHG9 was downregulated in OA and its expression was reversely correlated with the expression of miR-34a only across OA samples but not healthy control samples. In chondrocytes from OA patients, overexpression of SNHG9 led to downregulation of miR-34a and increased methylation of miR-34a gene. In contrast, in chondrocytes from healthy controls, overexpression of SNHG9 did not affect the expression of miR-34a and the methylation of miR-34a gene. Cell apoptosis analysis showed that overexpression of SNHG9 led to decreased apoptotic rate of chondrocytes from OA patients but not chondrocytes from the healthy controls through miR-34a. Conclusion In conclusion, SNHG9 is downregulated in OA and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation. |
| first_indexed | 2024-12-13T13:26:10Z |
| format | Article |
| id | doaj.art-2312865ddd584afaac13b09aa3a43185 |
| institution | Directory Open Access Journal |
| issn | 1471-2474 |
| language | English |
| last_indexed | 2024-12-13T13:26:10Z |
| publishDate | 2020-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj.art-2312865ddd584afaac13b09aa3a431852022-12-21T23:44:18ZengBMCBMC Musculoskeletal Disorders1471-24742020-08-012111710.1186/s12891-020-03497-7LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylationHongfei Zhang0Jinglian Li1Weiguang Shao2Naipeng Shen3Department of arthritis, Affiliated Hospital of Weifang Medical UniversityWeifang Medical UniversityDepartment of arthritis, Affiliated Hospital of Weifang Medical UniversityDepartment of arthritis, Affiliated Hospital of Weifang Medical UniversityAbstract Background Our preliminary RNA-Seq data revealed altered expression of small nucleolar RNA host gene 9 (SNHG9) in osteoarthritis (OA) and its reverse correlation with miR-34a, which can regulate chondrocyte apoptosis in rat OA model. This study was therefore carried out to investigate the potential interaction between SNHG9 and miR-34a in OA. Methods A total of 60 healthy volunteers (Control group) as well as 60 OA patients (OA group) were enrolled in this study. Transfections, RT-qPCR, methylation-specific PCR (MSP) and cell apoptosis assay were performed. Results We found that SNHG9 was downregulated in OA and its expression was reversely correlated with the expression of miR-34a only across OA samples but not healthy control samples. In chondrocytes from OA patients, overexpression of SNHG9 led to downregulation of miR-34a and increased methylation of miR-34a gene. In contrast, in chondrocytes from healthy controls, overexpression of SNHG9 did not affect the expression of miR-34a and the methylation of miR-34a gene. Cell apoptosis analysis showed that overexpression of SNHG9 led to decreased apoptotic rate of chondrocytes from OA patients but not chondrocytes from the healthy controls through miR-34a. Conclusion In conclusion, SNHG9 is downregulated in OA and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation.http://link.springer.com/article/10.1186/s12891-020-03497-7OsteoarthritisSNHG9miR-34aChondrocytesMethylation |
| spellingShingle | Hongfei Zhang Jinglian Li Weiguang Shao Naipeng Shen LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation BMC Musculoskeletal Disorders Osteoarthritis SNHG9 miR-34a Chondrocytes Methylation |
| title | LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation |
| title_full | LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation |
| title_fullStr | LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation |
| title_full_unstemmed | LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation |
| title_short | LncRNA SNHG9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating miR-34a through methylation |
| title_sort | lncrna snhg9 is downregulated in osteoarthritis and inhibits chondrocyte apoptosis by downregulating mir 34a through methylation |
| topic | Osteoarthritis SNHG9 miR-34a Chondrocytes Methylation |
| url | http://link.springer.com/article/10.1186/s12891-020-03497-7 |
| work_keys_str_mv | AT hongfeizhang lncrnasnhg9isdownregulatedinosteoarthritisandinhibitschondrocyteapoptosisbydownregulatingmir34athroughmethylation AT jinglianli lncrnasnhg9isdownregulatedinosteoarthritisandinhibitschondrocyteapoptosisbydownregulatingmir34athroughmethylation AT weiguangshao lncrnasnhg9isdownregulatedinosteoarthritisandinhibitschondrocyteapoptosisbydownregulatingmir34athroughmethylation AT naipengshen lncrnasnhg9isdownregulatedinosteoarthritisandinhibitschondrocyteapoptosisbydownregulatingmir34athroughmethylation |