Optimising protein synthesis in cell‐free systems, a review
Abstract Over the last decades, cell‐free systems have been extensively used for in vitro protein expression. A vast range of protocols and cellular sources varying from prokaryotes and eukaryotes are now available for cell‐free technology. However, exploiting the maximum capacity of cell free syste...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-03-01
|
| Series: | Engineering Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1049/enb2.12004 |
| _version_ | 1811231006803361792 |
|---|---|
| author | Angelo C. Batista Paul Soudier Manish Kushwaha Jean‐Loup Faulon |
| author_facet | Angelo C. Batista Paul Soudier Manish Kushwaha Jean‐Loup Faulon |
| author_sort | Angelo C. Batista |
| collection | DOAJ |
| description | Abstract Over the last decades, cell‐free systems have been extensively used for in vitro protein expression. A vast range of protocols and cellular sources varying from prokaryotes and eukaryotes are now available for cell‐free technology. However, exploiting the maximum capacity of cell free systems is not achieved by using traditional protocols. Here, what are the strategies and choices one can apply to optimise cell‐free protein synthesis have been reviewed. These strategies provide robust and informative improvements regarding transcription, translation and protein folding which can later be used for the establishment of individual best cell‐free reactions per lysate batch. |
| first_indexed | 2024-04-12T10:38:58Z |
| format | Article |
| id | doaj.art-231437a7d96d427eb5a4cc7399fd19d4 |
| institution | Directory Open Access Journal |
| issn | 2398-6182 |
| language | English |
| last_indexed | 2024-04-12T10:38:58Z |
| publishDate | 2021-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Engineering Biology |
| spelling | doaj.art-231437a7d96d427eb5a4cc7399fd19d42022-12-22T03:36:39ZengWileyEngineering Biology2398-61822021-03-0151101910.1049/enb2.12004Optimising protein synthesis in cell‐free systems, a reviewAngelo C. Batista0Paul Soudier1Manish Kushwaha2Jean‐Loup Faulon3Université Paris‐Saclay INRAE AgroParisTech Micalis Institute Jouy‐en‐Josas FranceUniversité Paris‐Saclay INRAE AgroParisTech Micalis Institute Jouy‐en‐Josas FranceUniversité Paris‐Saclay INRAE AgroParisTech Micalis Institute Jouy‐en‐Josas FranceUniversité Paris‐Saclay INRAE AgroParisTech Micalis Institute Jouy‐en‐Josas FranceAbstract Over the last decades, cell‐free systems have been extensively used for in vitro protein expression. A vast range of protocols and cellular sources varying from prokaryotes and eukaryotes are now available for cell‐free technology. However, exploiting the maximum capacity of cell free systems is not achieved by using traditional protocols. Here, what are the strategies and choices one can apply to optimise cell‐free protein synthesis have been reviewed. These strategies provide robust and informative improvements regarding transcription, translation and protein folding which can later be used for the establishment of individual best cell‐free reactions per lysate batch.https://doi.org/10.1049/enb2.12004molecular biophysicsbiochemistryproteinscellular biophysics |
| spellingShingle | Angelo C. Batista Paul Soudier Manish Kushwaha Jean‐Loup Faulon Optimising protein synthesis in cell‐free systems, a review Engineering Biology molecular biophysics biochemistry proteins cellular biophysics |
| title | Optimising protein synthesis in cell‐free systems, a review |
| title_full | Optimising protein synthesis in cell‐free systems, a review |
| title_fullStr | Optimising protein synthesis in cell‐free systems, a review |
| title_full_unstemmed | Optimising protein synthesis in cell‐free systems, a review |
| title_short | Optimising protein synthesis in cell‐free systems, a review |
| title_sort | optimising protein synthesis in cell free systems a review |
| topic | molecular biophysics biochemistry proteins cellular biophysics |
| url | https://doi.org/10.1049/enb2.12004 |
| work_keys_str_mv | AT angelocbatista optimisingproteinsynthesisincellfreesystemsareview AT paulsoudier optimisingproteinsynthesisincellfreesystemsareview AT manishkushwaha optimisingproteinsynthesisincellfreesystemsareview AT jeanloupfaulon optimisingproteinsynthesisincellfreesystemsareview |