Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size
Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It...
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Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo
2022-10-01
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Series: | Revista de la Facultad de Ciencias Agrarias |
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Online Access: | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/5668 |
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author | María Laura Foschi Liliana Estela Martínez María Teresa Ponce Claudio Rómulo Galmarini Borut Bohanec |
author_facet | María Laura Foschi Liliana Estela Martínez María Teresa Ponce Claudio Rómulo Galmarini Borut Bohanec |
author_sort | María Laura Foschi |
collection | DOAJ |
description |
Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It is necessary to adjust the concentration and the length of treatment of the doubling agent. In this case, the effect of 250 and 500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1 amiprophos-methyl during 24 and 48 h was assessed over the rate of onion haploid plantlets chromosome doubling. The best duplication treatment was 250 mg.L-1 colchicine for 48 h, which yielded 100% of doubled haploid plants. On the other hand, a positive correlation resulted from the ploidy level and stomatal size, and a negative correlation between the level of ploidy and stomatal density. Significant differences between the stomatal length, width and density in haploid and doubled haploid plantlets were observed. An economical and quick method to test ploidy level in onion plantlets is proposed through the measurement of stomatal size and density.
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first_indexed | 2024-03-12T17:47:17Z |
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id | doaj.art-2316dce360b24f378ac8630735c99748 |
institution | Directory Open Access Journal |
issn | 0370-4661 1853-8665 |
language | English |
last_indexed | 2024-03-12T17:47:17Z |
publishDate | 2022-10-01 |
publisher | Facultad de Ciencias Agrarias. Universidad Nacional de Cuyo |
record_format | Article |
series | Revista de la Facultad de Ciencias Agrarias |
spelling | doaj.art-2316dce360b24f378ac8630735c997482023-08-03T12:13:46ZengFacultad de Ciencias Agrarias. Universidad Nacional de CuyoRevista de la Facultad de Ciencias Agrarias0370-46611853-86652022-10-01452Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal sizeMaría Laura Foschi0Liliana Estela Martínez1María Teresa Ponce2Claudio Rómulo Galmarini3Borut Bohanec4Chair of Horticulture and Floriculture. Agricultural Faculty. National University of Cuyo. Alte. Brown 500. Chacras de Coria, Mendoza. Argentina. M5528AHB.Chair of Plant Physiology. Agricultural Faculty. National University of Cuyo. Instituto de Biología Agrícola de Mendoza-Consejo Nacional de Investigaciones Científicas y Técnicas (IBAM-CONICET). Alte. Brown 500, Chacras de Coria, Mendoza. Argentina. M5528AHB. Chair of Plant Physiology. Agricultural Faculty. National University of Cuyo. Instituto de Biología Agrícola de Mendoza-Consejo Nacional de Investigaciones Científicas y Técnicas (IBAM-CONICET). Alte. Brown 500, Chacras de Coria, Mendoza. Argentina. M5528AHB. Chair of Horticulture and Floriculture. Agricultural Faculty. National University of Cuyo. Alte. Brown 500. Chacras de Coria, Mendoza. Argentina. M5528AHB. Biotechnical Faculty. University of Ljubljana, Jamnikarjeva 101, 1000 Ljubljana, Slovenia. Doubled haploid onion (Allium cepa L.) plants allow the production of completely homozygous lines for a later production of hybrids. The haploid plants are normally produced using in vitro gynogenesis. The obtained haploid plantlets must be treated with different agents for doubling chromosomes. It is necessary to adjust the concentration and the length of treatment of the doubling agent. In this case, the effect of 250 and 500 mg.L-1 colchicine and 15.2; 30 and 60 mg.L- 1 amiprophos-methyl during 24 and 48 h was assessed over the rate of onion haploid plantlets chromosome doubling. The best duplication treatment was 250 mg.L-1 colchicine for 48 h, which yielded 100% of doubled haploid plants. On the other hand, a positive correlation resulted from the ploidy level and stomatal size, and a negative correlation between the level of ploidy and stomatal density. Significant differences between the stomatal length, width and density in haploid and doubled haploid plantlets were observed. An economical and quick method to test ploidy level in onion plantlets is proposed through the measurement of stomatal size and density. https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/5668Allium cepa L.antimitotic agentsduplicationgynogenesishybrids |
spellingShingle | María Laura Foschi Liliana Estela Martínez María Teresa Ponce Claudio Rómulo Galmarini Borut Bohanec Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size Revista de la Facultad de Ciencias Agrarias Allium cepa L. antimitotic agents duplication gynogenesis hybrids |
title | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
title_full | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
title_fullStr | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
title_full_unstemmed | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
title_short | Effect of colchicine and amiprophos-methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
title_sort | effect of colchicine and amiprophos methyl on the production of in vitro doubled haploid onion plants and correlation assessment between ploidy level and stomatal size |
topic | Allium cepa L. antimitotic agents duplication gynogenesis hybrids |
url | https://revistas.uncu.edu.ar/ojs/index.php/RFCA/article/view/5668 |
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