Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum
Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strai...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2024-02-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://ppjonline.org/upload/pdf/PPJ-FT-01-2024-0007.pdf |
| _version_ | 1797321011455066112 |
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| author | Yun-Seon Choi Da-Woon Kim Sung-Hwan Yun |
| author_facet | Yun-Seon Choi Da-Woon Kim Sung-Hwan Yun |
| author_sort | Yun-Seon Choi |
| collection | DOAJ |
| description | Fusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum. |
| first_indexed | 2024-03-08T04:51:21Z |
| format | Article |
| id | doaj.art-2318095746bc4165a00b2f51c17f6129 |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-03-08T04:51:21Z |
| publishDate | 2024-02-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-2318095746bc4165a00b2f51c17f61292024-02-08T05:01:26ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802024-02-01401839710.5423/PPJ.FT.01.2024.00072429Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearumYun-Seon Choi0Da-Woon Kim1Sung-Hwan Yun2 Department of Medical Biotechnology, Soonchunhyang University, Asan 31538, Korea Department of Medical Biotechnology, Soonchunhyang University, Asan 31538, Korea Department of Medical Biotechnology, Soonchunhyang University, Asan 31538, KoreaFusarium graminearum, the causal agent of Fusarium head blight (FHB) in cereal crops, employs the production of sexual fruiting bodies (perithecia) on plant debris as a strategy for overwintering and dissemination. In an artificial condition (e.g., carrot agar medium), the F. graminearum Z3643 strain was capable of producing perithecia predominantly in the central region of the fungal culture where aerial hyphae naturally collapsed. To unravel the intricate relationship between natural aerial hyphae collapse and sexual development in this fungus, we focused on 699 genes differentially expressed during aerial hyphae collapse, with 26 selected for further analysis. Targeted gene deletion and quantitative real-time PCR analyses elucidated the functions of specific genes during natural aerial hyphae collapse and perithecium formation. Furthermore, comparative gene expression analyses between natural collapse and artificial removal conditions reveal distinct temporal profiles, with the latter inducing a more rapid and pronounced response, particularly in MAT gene expression. Notably, FGSG_09210 and FGSG_09896 play crucial roles in sexual development and aerial hyphae growth, respectively. Taken together, it is plausible that if aerial hyphae collapse occurs on plant debris, it may serve as a physical cue for inducing perithecium formation in crop fields, representing a survival strategy for F. graminearum during winter. Insights into the molecular mechanisms underlying aerial hyphae collapse provides offer potential strategies for disease control against FHB caused by F. graminearum.http://ppjonline.org/upload/pdf/PPJ-FT-01-2024-0007.pdfaerial hyphae collapsefusarium graminearumfusarium head blightperithecium formation |
| spellingShingle | Yun-Seon Choi Da-Woon Kim Sung-Hwan Yun Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum The Plant Pathology Journal aerial hyphae collapse fusarium graminearum fusarium head blight perithecium formation |
| title | Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum |
| title_full | Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum |
| title_fullStr | Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum |
| title_full_unstemmed | Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum |
| title_short | Functional Analysis of Genes Specifically Expressed during Aerial Hyphae Collapse as a Potential Signal for Perithecium Formation Induction in Fusarium graminearum |
| title_sort | functional analysis of genes specifically expressed during aerial hyphae collapse as a potential signal for perithecium formation induction in fusarium graminearum |
| topic | aerial hyphae collapse fusarium graminearum fusarium head blight perithecium formation |
| url | http://ppjonline.org/upload/pdf/PPJ-FT-01-2024-0007.pdf |
| work_keys_str_mv | AT yunseonchoi functionalanalysisofgenesspecificallyexpressedduringaerialhyphaecollapseasapotentialsignalforperitheciumformationinductioninfusariumgraminearum AT dawoonkim functionalanalysisofgenesspecificallyexpressedduringaerialhyphaecollapseasapotentialsignalforperitheciumformationinductioninfusariumgraminearum AT sunghwanyun functionalanalysisofgenesspecificallyexpressedduringaerialhyphaecollapseasapotentialsignalforperitheciumformationinductioninfusariumgraminearum |