Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach
Anabolic steroids are widely abused in animal sports to improve their performance. The present study identifies the possible metabolites of boldenone in camel liver. A high-resolution accurate QE mass spectrometer was used to identify the parent boldenone and its metabolites. To investigate the pha...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-01-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/117992 |
| _version_ | 1811176066009530368 |
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| author | RAMEES ABDULLA VAZHAT N A MOHAMED FAROOK J NALAKATH PRASEEN O K |
| author_facet | RAMEES ABDULLA VAZHAT N A MOHAMED FAROOK J NALAKATH PRASEEN O K |
| author_sort | RAMEES ABDULLA VAZHAT |
| collection | DOAJ |
| description |
Anabolic steroids are widely abused in animal sports to improve their performance. The present study identifies the possible metabolites of boldenone in camel liver. A high-resolution accurate QE mass spectrometer was used to identify the parent boldenone and its metabolites. To investigate the phase 1 biotransformation of boldenone in camel, sodium phosphate buffer and NADPH were used. Chromatographic separation was carried out on a Thermo Hypersil C18 column using acetonitrile and formic acid as mobile phases. The current study, helped in unequivocal detection of six metabolites (Phase 1) for boldenone. The 17b-boldenone is an 3-oxo-Delta (1), Delta(4)-steroid substituted by an oxo group at position 3 and a beta-hydroxy group at position 17. It is prone to oxidation, which results in three hydroxylated metabolites with protonated parent ion of m/z 303.1954 (C19H27O3)+. Androsta-1,4-diene-3,17-dione [M+H]+ of m/z 285.1848 (C19H25O2+), hydroxyandrosta-1,4-diene-3,17-dione [M+H]+ of m/z 301.1797 (C19H25O3+) in addition to 17-hydroxy-androsta-1-en-3-one [M+H]+ of m/z 289.2162 (C19H29O2+) were also identified. The structures of the detected metabolites were identified based on their accurate mass, fragmentation pattern, and chromatographic retention time. In this research, camel liver was successfully used for in vitro experiments as an alternative to liver microsomes.
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| first_indexed | 2024-04-10T19:46:01Z |
| format | Article |
| id | doaj.art-2318ec85f19345ccaf513fd0c3ae1444 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T19:46:01Z |
| publishDate | 2023-01-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-2318ec85f19345ccaf513fd0c3ae14442023-01-29T04:35:32ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272023-01-0193110.56093/ijans.v93i1.117992Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approachRAMEES ABDULLA VAZHAT0N A MOHAMED FAROOK1J NALAKATH2PRASEEN O K3Central Veterinary Research Laboratory, PO Box 597, Dubai, United Arab EmiratesKhadir Mohideen College, Bharathidasan University, Adirampattinam, Tamil NaduCentral Veterinary Research Laboratory, PO Box 597, Dubai, United Arab EmiratesCentral Veterinary Research Laboratory, PO Box 597, Dubai, United Arab Emirates Anabolic steroids are widely abused in animal sports to improve their performance. The present study identifies the possible metabolites of boldenone in camel liver. A high-resolution accurate QE mass spectrometer was used to identify the parent boldenone and its metabolites. To investigate the phase 1 biotransformation of boldenone in camel, sodium phosphate buffer and NADPH were used. Chromatographic separation was carried out on a Thermo Hypersil C18 column using acetonitrile and formic acid as mobile phases. The current study, helped in unequivocal detection of six metabolites (Phase 1) for boldenone. The 17b-boldenone is an 3-oxo-Delta (1), Delta(4)-steroid substituted by an oxo group at position 3 and a beta-hydroxy group at position 17. It is prone to oxidation, which results in three hydroxylated metabolites with protonated parent ion of m/z 303.1954 (C19H27O3)+. Androsta-1,4-diene-3,17-dione [M+H]+ of m/z 285.1848 (C19H25O2+), hydroxyandrosta-1,4-diene-3,17-dione [M+H]+ of m/z 301.1797 (C19H25O3+) in addition to 17-hydroxy-androsta-1-en-3-one [M+H]+ of m/z 289.2162 (C19H29O2+) were also identified. The structures of the detected metabolites were identified based on their accurate mass, fragmentation pattern, and chromatographic retention time. In this research, camel liver was successfully used for in vitro experiments as an alternative to liver microsomes. https://epubs.icar.org.in/index.php/IJAnS/article/view/117992Anabolic steroidsBiotransformationBoldenoneCamel liverIn vitro metabolismHRMS |
| spellingShingle | RAMEES ABDULLA VAZHAT N A MOHAMED FAROOK J NALAKATH PRASEEN O K Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach Indian Journal of Animal Sciences Anabolic steroids Biotransformation Boldenone Camel liver In vitro metabolism HRMS |
| title | Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach |
| title_full | Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach |
| title_fullStr | Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach |
| title_full_unstemmed | Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach |
| title_short | Identification of in vitro metabolites of boldenone using Camel liver microsomes – A HR-LCMS approach |
| title_sort | identification of in vitro metabolites of boldenone using camel liver microsomes a hr lcms approach |
| topic | Anabolic steroids Biotransformation Boldenone Camel liver In vitro metabolism HRMS |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/117992 |
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