The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts
This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/20/12360 |
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| author | Magda Aleksandra Pawlicka Szymon Zmorzyński Sylwia Popek-Marciniec Agata Anna Filip |
| author_facet | Magda Aleksandra Pawlicka Szymon Zmorzyński Sylwia Popek-Marciniec Agata Anna Filip |
| author_sort | Magda Aleksandra Pawlicka |
| collection | DOAJ |
| description | This study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy isoflavonoids on <i>MCF-7</i> tumor cells and <i>BJ</i> skin fibroblasts cultured in vitro were assessed. Genistein affects both healthy dermal <i>BJ</i> fibroblasts and cancerous <i>MCF-7</i> cells. The effect of the tested isoflavonoid is closely related to its concentration. High concentrations of genistein destroy <i>MCF-7</i> cancer cells, regardless of the exposure time, with a much greater effect on reducing cancer cell numbers at longer times (48 h). Lower concentrations of genistein (10 and 20 μM) increase the abundance of dermal fibroblasts. However, higher concentrations of genistein (50 μM and higher) are detrimental to fibroblasts at longer exposure times (48 h). Our studies indicate that although genistein shows high potential for use in the treatment of skin problems, wounds and surgical scars in women during and after breast cancer treatment, it is not completely safe. Introducing isoflavonoids to treatment requires further research into their mechanisms of action at the molecular level, taking into account genetic and immunological aspects. It is also necessary to conduct research in in vivo models, which will allow for eliminating adverse side effects of therapy. |
| first_indexed | 2024-03-09T09:46:37Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:46:37Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2319bf9a9e5e43a3b6c5124f4481aaf22023-12-02T00:32:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123201236010.3390/ijms232012360The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal FibroblastsMagda Aleksandra Pawlicka0Szymon Zmorzyński1Sylwia Popek-Marciniec2Agata Anna Filip3Department of Cancer Genetics with Cytogenetic Laboratory, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, PolandDepartment of Cancer Genetics with Cytogenetic Laboratory, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, PolandDepartment of Cancer Genetics with Cytogenetic Laboratory, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, PolandDepartment of Cancer Genetics with Cytogenetic Laboratory, Medical University of Lublin, Radziwiłłowska 11 Street, 20-080 Lublin, PolandThis study aimed to evaluate the safety and potential use of soy isoflavones in the treatment of skin problems, difficult-to-heal wounds and postoperative scars in women after the oncological treatment of breast cancer. The effects of different concentrations of genistein as a representative of soy isoflavonoids on <i>MCF-7</i> tumor cells and <i>BJ</i> skin fibroblasts cultured in vitro were assessed. Genistein affects both healthy dermal <i>BJ</i> fibroblasts and cancerous <i>MCF-7</i> cells. The effect of the tested isoflavonoid is closely related to its concentration. High concentrations of genistein destroy <i>MCF-7</i> cancer cells, regardless of the exposure time, with a much greater effect on reducing cancer cell numbers at longer times (48 h). Lower concentrations of genistein (10 and 20 μM) increase the abundance of dermal fibroblasts. However, higher concentrations of genistein (50 μM and higher) are detrimental to fibroblasts at longer exposure times (48 h). Our studies indicate that although genistein shows high potential for use in the treatment of skin problems, wounds and surgical scars in women during and after breast cancer treatment, it is not completely safe. Introducing isoflavonoids to treatment requires further research into their mechanisms of action at the molecular level, taking into account genetic and immunological aspects. It is also necessary to conduct research in in vivo models, which will allow for eliminating adverse side effects of therapy.https://www.mdpi.com/1422-0067/23/20/12360genisteinbreast cancer<i>MCF-7</i> cellsskinfibroblasts |
| spellingShingle | Magda Aleksandra Pawlicka Szymon Zmorzyński Sylwia Popek-Marciniec Agata Anna Filip The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts International Journal of Molecular Sciences genistein breast cancer <i>MCF-7</i> cells skin fibroblasts |
| title | The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts |
| title_full | The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts |
| title_fullStr | The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts |
| title_full_unstemmed | The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts |
| title_short | The Effects of Genistein at Different Concentrations on <i>MCF-7</i> Breast Cancer Cells and <i>BJ</i> Dermal Fibroblasts |
| title_sort | effects of genistein at different concentrations on i mcf 7 i breast cancer cells and i bj i dermal fibroblasts |
| topic | genistein breast cancer <i>MCF-7</i> cells skin fibroblasts |
| url | https://www.mdpi.com/1422-0067/23/20/12360 |
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