Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice.
Rift Valley fever (RVF) in humans is usually mild, but, in a subset of cases, can progress to severe hepatic and neurological disease. Rodent models of RVF generally develop acute severe clinical disease. Here, we inoculated humanized NSG-SGM3 mice with Rift Valley fever virus (RVFV) to investigate...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6054394?pdf=render |
| _version_ | 1819274124467372032 |
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| author | Jessica R Spengler Anita K McElroy Jessica R Harmon JoAnn D Coleman-McCray Stephen R Welch James G Keck Stuart T Nichol Christina F Spiropoulou |
| author_facet | Jessica R Spengler Anita K McElroy Jessica R Harmon JoAnn D Coleman-McCray Stephen R Welch James G Keck Stuart T Nichol Christina F Spiropoulou |
| author_sort | Jessica R Spengler |
| collection | DOAJ |
| description | Rift Valley fever (RVF) in humans is usually mild, but, in a subset of cases, can progress to severe hepatic and neurological disease. Rodent models of RVF generally develop acute severe clinical disease. Here, we inoculated humanized NSG-SGM3 mice with Rift Valley fever virus (RVFV) to investigate whether the presence of human immune cells in mice would alter the progression of RVFV infection to more closely model human disease. Despite increased human cytokine expression, including responses mirroring those seen in human disease, and decreased hepatic viral RNA levels at terminal euthanasia, both high- and low-dose RVFV inoculation resulted in lethal disease in all mice with comparable time-to-death as unengrafted mice. |
| first_indexed | 2024-12-23T23:03:26Z |
| format | Article |
| id | doaj.art-2320d56b25624824b745bee101050b66 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T23:03:26Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2320d56b25624824b745bee101050b662022-12-21T17:26:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01137e020110410.1371/journal.pone.0201104Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice.Jessica R SpenglerAnita K McElroyJessica R HarmonJoAnn D Coleman-McCrayStephen R WelchJames G KeckStuart T NicholChristina F SpiropoulouRift Valley fever (RVF) in humans is usually mild, but, in a subset of cases, can progress to severe hepatic and neurological disease. Rodent models of RVF generally develop acute severe clinical disease. Here, we inoculated humanized NSG-SGM3 mice with Rift Valley fever virus (RVFV) to investigate whether the presence of human immune cells in mice would alter the progression of RVFV infection to more closely model human disease. Despite increased human cytokine expression, including responses mirroring those seen in human disease, and decreased hepatic viral RNA levels at terminal euthanasia, both high- and low-dose RVFV inoculation resulted in lethal disease in all mice with comparable time-to-death as unengrafted mice.http://europepmc.org/articles/PMC6054394?pdf=render |
| spellingShingle | Jessica R Spengler Anita K McElroy Jessica R Harmon JoAnn D Coleman-McCray Stephen R Welch James G Keck Stuart T Nichol Christina F Spiropoulou Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice. PLoS ONE |
| title | Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice. |
| title_full | Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice. |
| title_fullStr | Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice. |
| title_full_unstemmed | Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice. |
| title_short | Human immune cell engraftment does not alter development of severe acute Rift Valley fever in mice. |
| title_sort | human immune cell engraftment does not alter development of severe acute rift valley fever in mice |
| url | http://europepmc.org/articles/PMC6054394?pdf=render |
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