A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>
Fusarium wilt of banana (also known as Panama disease), is a severe fungal disease caused by soil-borne <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>). In recent years, biocontrol strategies using antifungal microorganisms from various niches and the...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/9/516 |
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| author | Pratiksha Singh Jin Xie Yanhua Qi Qijian Qin Cheng Jin Bin Wang Wenxia Fang |
| author_facet | Pratiksha Singh Jin Xie Yanhua Qi Qijian Qin Cheng Jin Bin Wang Wenxia Fang |
| author_sort | Pratiksha Singh |
| collection | DOAJ |
| description | Fusarium wilt of banana (also known as Panama disease), is a severe fungal disease caused by soil-borne <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>). In recent years, biocontrol strategies using antifungal microorganisms from various niches and their related bioactive compounds have been used to prevent and control Panama disease. Here, a thermotolerant marine strain S185 was identified as <i>Bacillus amyloliquefaciens</i>, displaying strong antifungal activity against <i>Foc</i>. The strain S185 possesses multiple plant growth-promoting (PGP) and biocontrol utility properties, such as producing indole acetic acid (IAA) and ammonia, assimilating various carbon sources, tolerating pH of 4 to 9, temperature of 20 to 50 °C, and salt stress of 1 to 5%. Inoculation of S185 colonized the banana plants effectively and was mainly located in leaf and root tissues. To further investigate the antifungal components, compounds were extracted, fractionated, and purified. One compound, inhibiting <i>Foc</i> with minimum inhibitory concentrations (MICs) of 25 μg/disk, was identified as iturin A5 by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR). The isolated iturin, A5, resulted in severe morphological changes during spore germination and hyphae growth of <i>Foc</i>. These results specify that <i>B. amyloliquefaciens</i> S185 plays a key role in preventing the <i>Foc</i> pathogen by producing the antifungal compound iturin A5, and possesses potential as a cost-effective and sustainable biocontrol strain for Panama disease in the future. This is the first report of isolation of the antifungal compound iturin A5 from thermotolerant marine <i>B. amyloliquefaciens</i> S185. |
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| language | English |
| last_indexed | 2024-03-10T07:29:43Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-232168319be54c628d771e686f238c702023-11-22T13:58:58ZengMDPI AGMarine Drugs1660-33972021-09-0119951610.3390/md19090516A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i>Pratiksha Singh0Jin Xie1Yanhua Qi2Qijian Qin3Cheng Jin4Bin Wang5Wenxia Fang6State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaState Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaFusarium wilt of banana (also known as Panama disease), is a severe fungal disease caused by soil-borne <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (<i>Foc</i>). In recent years, biocontrol strategies using antifungal microorganisms from various niches and their related bioactive compounds have been used to prevent and control Panama disease. Here, a thermotolerant marine strain S185 was identified as <i>Bacillus amyloliquefaciens</i>, displaying strong antifungal activity against <i>Foc</i>. The strain S185 possesses multiple plant growth-promoting (PGP) and biocontrol utility properties, such as producing indole acetic acid (IAA) and ammonia, assimilating various carbon sources, tolerating pH of 4 to 9, temperature of 20 to 50 °C, and salt stress of 1 to 5%. Inoculation of S185 colonized the banana plants effectively and was mainly located in leaf and root tissues. To further investigate the antifungal components, compounds were extracted, fractionated, and purified. One compound, inhibiting <i>Foc</i> with minimum inhibitory concentrations (MICs) of 25 μg/disk, was identified as iturin A5 by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR). The isolated iturin, A5, resulted in severe morphological changes during spore germination and hyphae growth of <i>Foc</i>. These results specify that <i>B. amyloliquefaciens</i> S185 plays a key role in preventing the <i>Foc</i> pathogen by producing the antifungal compound iturin A5, and possesses potential as a cost-effective and sustainable biocontrol strain for Panama disease in the future. This is the first report of isolation of the antifungal compound iturin A5 from thermotolerant marine <i>B. amyloliquefaciens</i> S185.https://www.mdpi.com/1660-3397/19/9/516antifungal activity<i>Bacillus amyloliquefaciens</i>Panama disease<i>Fusarium oxysporum</i> f. sp. <i>cubense</i>bioactive compounditurin A5 |
| spellingShingle | Pratiksha Singh Jin Xie Yanhua Qi Qijian Qin Cheng Jin Bin Wang Wenxia Fang A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> Marine Drugs antifungal activity <i>Bacillus amyloliquefaciens</i> Panama disease <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> bioactive compound iturin A5 |
| title | A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_full | A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_fullStr | A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_full_unstemmed | A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_short | A Thermotolerant Marine <i>Bacillus amyloliquefaciens</i> S185 Producing Iturin A5 for Antifungal Activity against <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> |
| title_sort | thermotolerant marine i bacillus amyloliquefaciens i s185 producing iturin a5 for antifungal activity against i fusarium oxysporum i f sp i cubense i |
| topic | antifungal activity <i>Bacillus amyloliquefaciens</i> Panama disease <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> bioactive compound iturin A5 |
| url | https://www.mdpi.com/1660-3397/19/9/516 |
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