Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique.
Syringomyelia (SM) is a spinal cord disorder in which a cyst (syrinx) filled with fluid forms in the spinal cord post-injury/disease, in patients syrinx symptoms include loss of pain and temperature sensation or locomotion deficit. Currently, there are no small animal models and connected tools to h...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0252559 |
| _version_ | 1819003935259623424 |
|---|---|
| author | Dipak D Pukale Mahmoud Farrag Nic D Leipzig |
| author_facet | Dipak D Pukale Mahmoud Farrag Nic D Leipzig |
| author_sort | Dipak D Pukale |
| collection | DOAJ |
| description | Syringomyelia (SM) is a spinal cord disorder in which a cyst (syrinx) filled with fluid forms in the spinal cord post-injury/disease, in patients syrinx symptoms include loss of pain and temperature sensation or locomotion deficit. Currently, there are no small animal models and connected tools to help study the functional impacts of SM. The objective of this study was to determine the detectability of subtle locomotion deficits due to syrinx formation/expansion in post-traumatic syringomyelia (PTSM) rat model using the recently reported method of Gait Analysis Instrumentation, and Technology Optimized for Rodents (GAITOR) with Automated Gait Analysis Through Hues and Areas (AGATHA) technique. First videos of the rats were collected while walking in an arena (using GAITOR) followed by extracting meaningful locomotion information from collected videos using AGATHA protocol. PTSM injured rats demonstrated detectable locomotion deficits in terms of duty factor imbalance, paw placement accuracy, step contact width, stride length, and phase dispersion parameters compared to uninjured rats due to SM. We concluded that this technique could detect mild and subtle locomotion deficits associated with PTSM injury, which also in future work could be used further to monitor locomotion responses after different treatment strategies for SM. |
| first_indexed | 2024-12-20T23:28:54Z |
| format | Article |
| id | doaj.art-232181792c104f7ebd00c91e484f31b0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T23:28:54Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-232181792c104f7ebd00c91e484f31b02022-12-21T19:23:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011611e025255910.1371/journal.pone.0252559Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique.Dipak D PukaleMahmoud FarragNic D LeipzigSyringomyelia (SM) is a spinal cord disorder in which a cyst (syrinx) filled with fluid forms in the spinal cord post-injury/disease, in patients syrinx symptoms include loss of pain and temperature sensation or locomotion deficit. Currently, there are no small animal models and connected tools to help study the functional impacts of SM. The objective of this study was to determine the detectability of subtle locomotion deficits due to syrinx formation/expansion in post-traumatic syringomyelia (PTSM) rat model using the recently reported method of Gait Analysis Instrumentation, and Technology Optimized for Rodents (GAITOR) with Automated Gait Analysis Through Hues and Areas (AGATHA) technique. First videos of the rats were collected while walking in an arena (using GAITOR) followed by extracting meaningful locomotion information from collected videos using AGATHA protocol. PTSM injured rats demonstrated detectable locomotion deficits in terms of duty factor imbalance, paw placement accuracy, step contact width, stride length, and phase dispersion parameters compared to uninjured rats due to SM. We concluded that this technique could detect mild and subtle locomotion deficits associated with PTSM injury, which also in future work could be used further to monitor locomotion responses after different treatment strategies for SM.https://doi.org/10.1371/journal.pone.0252559 |
| spellingShingle | Dipak D Pukale Mahmoud Farrag Nic D Leipzig Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique. PLoS ONE |
| title | Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique. |
| title_full | Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique. |
| title_fullStr | Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique. |
| title_full_unstemmed | Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique. |
| title_short | Detection of locomotion deficit in a post-traumatic syringomyelia rat model using automated gait analysis technique. |
| title_sort | detection of locomotion deficit in a post traumatic syringomyelia rat model using automated gait analysis technique |
| url | https://doi.org/10.1371/journal.pone.0252559 |
| work_keys_str_mv | AT dipakdpukale detectionoflocomotiondeficitinaposttraumaticsyringomyeliaratmodelusingautomatedgaitanalysistechnique AT mahmoudfarrag detectionoflocomotiondeficitinaposttraumaticsyringomyeliaratmodelusingautomatedgaitanalysistechnique AT nicdleipzig detectionoflocomotiondeficitinaposttraumaticsyringomyeliaratmodelusingautomatedgaitanalysistechnique |