Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada
Antimicrobial resistance (AMR) is an important burden for public health and veterinary medicine. For Québec (Canada) dairy farms, the prevalence of AMR is mostly described using passive surveillance, which may be misleading. In addition, the presence of extended spectrum β-lactamase (ESBL)/AmpC prod...
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2021.654125/full |
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| author | Jonathan Massé Jonathan Massé Jonathan Massé Hélène Lardé Hélène Lardé John M. Fairbrother John M. Fairbrother Jean-Philippe Roy Jean-Philippe Roy David Francoz David Francoz Simon Dufour Simon Dufour Marie Archambault Marie Archambault Marie Archambault |
| author_facet | Jonathan Massé Jonathan Massé Jonathan Massé Hélène Lardé Hélène Lardé John M. Fairbrother John M. Fairbrother Jean-Philippe Roy Jean-Philippe Roy David Francoz David Francoz Simon Dufour Simon Dufour Marie Archambault Marie Archambault Marie Archambault |
| author_sort | Jonathan Massé |
| collection | DOAJ |
| description | Antimicrobial resistance (AMR) is an important burden for public health and veterinary medicine. For Québec (Canada) dairy farms, the prevalence of AMR is mostly described using passive surveillance, which may be misleading. In addition, the presence of extended spectrum β-lactamase (ESBL)/AmpC producing Escherichia coli is unknown. This observational cross-sectional study used random dairy farms (n = 101) to investigate AMR and extended spectrum β-lactamase (ESBL)/AmpC producing Escherichia coli. Twenty antimicrobials were tested on E. coli isolates (n = 593) recovered from fecal samples (n = 599) from calves, cows, and the manure pit. Isolates were mostly susceptible (3% AMR or less) to the highest priority critically important antimicrobials in humans. The highest levels of AMR were to tetracycline (26%), sulfisozaxole (23%) and streptomycin (19%). The resistance genes responsible for these resistances were, respectively: tet(A), tet(B), sul1, sul2, sul3, aph(3”)-Ib (strA), aph(6)-Id (strB), aadA1, aadA2, and aadA5. ESBL analysis revealed two predominant phenotypes: AmpC (51%) and ESBL (46%) where blaCMY−2 and blaCTX−M(blaCTX−M−1, blaCTX−M−15, and blaCTX−M−55) were the genes responsible for these phenotypes, respectively. During this study, 85% of farms had at least one ESBL/AmpC producing E. coli. Isolates from calves were more frequently resistant than those from cows or manure pits. Although prevalence of AMR was low for critically important antimicrobials, there was a high prevalence of ESBL/AmpC-producing E. coli on Quebec dairy farms, particularly in calves. Those data will help determine a baseline for AMR to evaluate impact of initiatives aimed at reducing AMR. |
| first_indexed | 2024-12-21T15:48:23Z |
| format | Article |
| id | doaj.art-2323eb56a1614588bad8f3eaceab3699 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-21T15:48:23Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Veterinary Science |
| spelling | doaj.art-2323eb56a1614588bad8f3eaceab36992022-12-21T18:58:17ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692021-05-01810.3389/fvets.2021.654125654125Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, CanadaJonathan Massé0Jonathan Massé1Jonathan Massé2Hélène Lardé3Hélène Lardé4John M. Fairbrother5John M. Fairbrother6Jean-Philippe Roy7Jean-Philippe Roy8David Francoz9David Francoz10Simon Dufour11Simon Dufour12Marie Archambault13Marie Archambault14Marie Archambault15Regroupement FRQNT Op+lait, Saint-Hyacinthe, QC, CanadaGroupe de Recherche sur les maladies infectieuses en production animale, Saint-Hyacinthe, QC, CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC, CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaGroupe de Recherche sur les maladies infectieuses en production animale, Saint-Hyacinthe, QC, CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC, CanadaDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC, CanadaDepartment of Clinical Sciences, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC, CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaRegroupement FRQNT Op+lait, Saint-Hyacinthe, QC, CanadaGroupe de Recherche sur les maladies infectieuses en production animale, Saint-Hyacinthe, QC, CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, Saint-Hyacinthe, QC, CanadaAntimicrobial resistance (AMR) is an important burden for public health and veterinary medicine. For Québec (Canada) dairy farms, the prevalence of AMR is mostly described using passive surveillance, which may be misleading. In addition, the presence of extended spectrum β-lactamase (ESBL)/AmpC producing Escherichia coli is unknown. This observational cross-sectional study used random dairy farms (n = 101) to investigate AMR and extended spectrum β-lactamase (ESBL)/AmpC producing Escherichia coli. Twenty antimicrobials were tested on E. coli isolates (n = 593) recovered from fecal samples (n = 599) from calves, cows, and the manure pit. Isolates were mostly susceptible (3% AMR or less) to the highest priority critically important antimicrobials in humans. The highest levels of AMR were to tetracycline (26%), sulfisozaxole (23%) and streptomycin (19%). The resistance genes responsible for these resistances were, respectively: tet(A), tet(B), sul1, sul2, sul3, aph(3”)-Ib (strA), aph(6)-Id (strB), aadA1, aadA2, and aadA5. ESBL analysis revealed two predominant phenotypes: AmpC (51%) and ESBL (46%) where blaCMY−2 and blaCTX−M(blaCTX−M−1, blaCTX−M−15, and blaCTX−M−55) were the genes responsible for these phenotypes, respectively. During this study, 85% of farms had at least one ESBL/AmpC producing E. coli. Isolates from calves were more frequently resistant than those from cows or manure pits. Although prevalence of AMR was low for critically important antimicrobials, there was a high prevalence of ESBL/AmpC-producing E. coli on Quebec dairy farms, particularly in calves. Those data will help determine a baseline for AMR to evaluate impact of initiatives aimed at reducing AMR.https://www.frontiersin.org/articles/10.3389/fvets.2021.654125/fullantimicrobial resistanceEscherichia coliESBL/AmpCfecaldairy cattlecalf |
| spellingShingle | Jonathan Massé Jonathan Massé Jonathan Massé Hélène Lardé Hélène Lardé John M. Fairbrother John M. Fairbrother Jean-Philippe Roy Jean-Philippe Roy David Francoz David Francoz Simon Dufour Simon Dufour Marie Archambault Marie Archambault Marie Archambault Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada Frontiers in Veterinary Science antimicrobial resistance Escherichia coli ESBL/AmpC fecal dairy cattle calf |
| title | Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada |
| title_full | Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada |
| title_fullStr | Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada |
| title_full_unstemmed | Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada |
| title_short | Prevalence of Antimicrobial Resistance and Characteristics of Escherichia coli Isolates From Fecal and Manure Pit Samples on Dairy Farms in the Province of Québec, Canada |
| title_sort | prevalence of antimicrobial resistance and characteristics of escherichia coli isolates from fecal and manure pit samples on dairy farms in the province of quebec canada |
| topic | antimicrobial resistance Escherichia coli ESBL/AmpC fecal dairy cattle calf |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2021.654125/full |
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