DNA damage, oxidative stress, and inflammation in children with celiac disease
Abstract The objective of this study was to evaluate the level of genomic instability in patients with celiac disease and to establish a relationship between inflammation, oxidative stress, and DNA damage in these patients. Myeloperoxidase (MPO) activity, adenosine deaminase, nitric oxide (NOx), thi...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
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| Series: | Genetics and Molecular Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400112&lng=en&tlng=en |
| _version_ | 1819013799742537728 |
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| author | Sharbel Weidner Maluf Danilo Wilhelm Filho Eduardo Benedetti Parisotto Guilherme da Silva de Medeiros Carolina Hilgert Jacobsen Pereira Flora Troina Maraslis Carlos C. Dornelles Schoeller Julia Savan da Rosa Tânia Silvia Fröde |
| author_facet | Sharbel Weidner Maluf Danilo Wilhelm Filho Eduardo Benedetti Parisotto Guilherme da Silva de Medeiros Carolina Hilgert Jacobsen Pereira Flora Troina Maraslis Carlos C. Dornelles Schoeller Julia Savan da Rosa Tânia Silvia Fröde |
| author_sort | Sharbel Weidner Maluf |
| collection | DOAJ |
| description | Abstract The objective of this study was to evaluate the level of genomic instability in patients with celiac disease and to establish a relationship between inflammation, oxidative stress, and DNA damage in these patients. Myeloperoxidase (MPO) activity, adenosine deaminase, nitric oxide (NOx), thiobarbituric acid, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and DNA damage were evaluated in peripheral blood samples from 47 celiac disease patients and 31 controls. Patients with celiac disease presented higher levels of DNA damage in comparison to controls (p=0.023). This difference was also observed for markers of oxidative stress, such as CAT (p=0.011) and SOD (p=0.013), and inflammatory markers such as MPO (p < 0.001) and NOx (p=0.009). Positive correlations were found between DNA damage levels and the values of CAT (r=0.405; p=0.009) and SOD (r=0.516; p < 0.001). Positive correlations were also found between GPx and NOx (r=0.349; p=0.030) and MPO and NOx (r=0.239; p=0.039). CAT and NOx showed a negative correlation (r= −0.315; p=0.042). In conclusion, intestinal inflammation can have systemic effects, causing an imbalance between oxidant and antioxidant markers, which may promote increased levels of DNA damage. |
| first_indexed | 2024-12-21T02:05:41Z |
| format | Article |
| id | doaj.art-232729f7eb4542f7bf9ac77030dd23d5 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T02:05:41Z |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-232729f7eb4542f7bf9ac77030dd23d52022-12-21T19:19:29ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-468543210.1590/1678-4685-gmb-2018-0390S1415-47572020000400112DNA damage, oxidative stress, and inflammation in children with celiac diseaseSharbel Weidner MalufDanilo Wilhelm FilhoEduardo Benedetti ParisottoGuilherme da Silva de MedeirosCarolina Hilgert Jacobsen PereiraFlora Troina MaraslisCarlos C. Dornelles SchoellerJulia Savan da RosaTânia Silvia FrödeAbstract The objective of this study was to evaluate the level of genomic instability in patients with celiac disease and to establish a relationship between inflammation, oxidative stress, and DNA damage in these patients. Myeloperoxidase (MPO) activity, adenosine deaminase, nitric oxide (NOx), thiobarbituric acid, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and DNA damage were evaluated in peripheral blood samples from 47 celiac disease patients and 31 controls. Patients with celiac disease presented higher levels of DNA damage in comparison to controls (p=0.023). This difference was also observed for markers of oxidative stress, such as CAT (p=0.011) and SOD (p=0.013), and inflammatory markers such as MPO (p < 0.001) and NOx (p=0.009). Positive correlations were found between DNA damage levels and the values of CAT (r=0.405; p=0.009) and SOD (r=0.516; p < 0.001). Positive correlations were also found between GPx and NOx (r=0.349; p=0.030) and MPO and NOx (r=0.239; p=0.039). CAT and NOx showed a negative correlation (r= −0.315; p=0.042). In conclusion, intestinal inflammation can have systemic effects, causing an imbalance between oxidant and antioxidant markers, which may promote increased levels of DNA damage.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400112&lng=en&tlng=enceliac diseasebiochemical markersgenotoxicityreactive oxygen species (ros)inflammatory markers |
| spellingShingle | Sharbel Weidner Maluf Danilo Wilhelm Filho Eduardo Benedetti Parisotto Guilherme da Silva de Medeiros Carolina Hilgert Jacobsen Pereira Flora Troina Maraslis Carlos C. Dornelles Schoeller Julia Savan da Rosa Tânia Silvia Fröde DNA damage, oxidative stress, and inflammation in children with celiac disease Genetics and Molecular Biology celiac disease biochemical markers genotoxicity reactive oxygen species (ros) inflammatory markers |
| title | DNA damage, oxidative stress, and inflammation in children with celiac disease |
| title_full | DNA damage, oxidative stress, and inflammation in children with celiac disease |
| title_fullStr | DNA damage, oxidative stress, and inflammation in children with celiac disease |
| title_full_unstemmed | DNA damage, oxidative stress, and inflammation in children with celiac disease |
| title_short | DNA damage, oxidative stress, and inflammation in children with celiac disease |
| title_sort | dna damage oxidative stress and inflammation in children with celiac disease |
| topic | celiac disease biochemical markers genotoxicity reactive oxygen species (ros) inflammatory markers |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000400112&lng=en&tlng=en |
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