Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i>
Grapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive<sup>®</sup> (a <...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/5/996 |
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| author | Pedro Reis Vincenzo Mondello Inês Diniz Artur Alves Cecília Rego Florence Fontaine |
| author_facet | Pedro Reis Vincenzo Mondello Inês Diniz Artur Alves Cecília Rego Florence Fontaine |
| author_sort | Pedro Reis |
| collection | DOAJ |
| description | Grapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive<sup>®</sup> (a <i>Trichoderma</i>-based product) and LC2017 (a low-copper-based product), in the control of <i>Lasiodiplodia theobromae</i>, by evaluating the internal lesion length caused by inoculation of this pathogen on greenhouse kept grapevines of cvs Cabernet Sauvignon and Touriga Nacional and, (ii) investigating their elicitor effect on plant defense responses, through the analysis of the expression of a set of genes. The pathogen was always re-isolated from the infected tissues and was able to cause wood discoloration. Touriga Nacional exhibited longer lesions than Cabernet Sauvignon, and the application of both products did not appear to reduce lesion length when compared to LC2017 applied alone. The elicitor effect of LC2017 on plant defense was confirmed by gene expression analysis, and no significant differences were found between plants treated with LC2017 and with both products. Moreover, a specific response related to the cultivar was verified, but this apparently unique interaction between product, cultivar and pathogen remains to be further investigated. |
| first_indexed | 2024-03-10T03:31:03Z |
| format | Article |
| id | doaj.art-232d9c47713e4379a84c3af0fc3327d1 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:31:03Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-232d9c47713e4379a84c3af0fc3327d12023-11-23T09:41:16ZengMDPI AGAgronomy2073-43952022-04-0112599610.3390/agronomy12050996Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i>Pedro Reis0Vincenzo Mondello1Inês Diniz2Artur Alves3Cecília Rego4Florence Fontaine5LEAF—Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, PortugalUnité Résistance Induite et Bioprotection des Plantes, URCA—Université de Reims Champagne-Ardenne, EA 4707 USC INRAE 1488, SFR Condorcet FR CNRS 3417, 51687 Reims, FranceLEAF—Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, PortugalCESAM—Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, 3810-193 Aveiro, PortugalLEAF—Linking Landscape, Environment, Agriculture and Food-Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisbon, PortugalUnité Résistance Induite et Bioprotection des Plantes, URCA—Université de Reims Champagne-Ardenne, EA 4707 USC INRAE 1488, SFR Condorcet FR CNRS 3417, 51687 Reims, FranceGrapevine trunk diseases constitute one of the major problems for viticulture worldwide, with Botryosphaeria dieback considered as one of the most important of these diseases. In this work, we aimed to (i) evaluate the effect of the combination of two products, Esquive<sup>®</sup> (a <i>Trichoderma</i>-based product) and LC2017 (a low-copper-based product), in the control of <i>Lasiodiplodia theobromae</i>, by evaluating the internal lesion length caused by inoculation of this pathogen on greenhouse kept grapevines of cvs Cabernet Sauvignon and Touriga Nacional and, (ii) investigating their elicitor effect on plant defense responses, through the analysis of the expression of a set of genes. The pathogen was always re-isolated from the infected tissues and was able to cause wood discoloration. Touriga Nacional exhibited longer lesions than Cabernet Sauvignon, and the application of both products did not appear to reduce lesion length when compared to LC2017 applied alone. The elicitor effect of LC2017 on plant defense was confirmed by gene expression analysis, and no significant differences were found between plants treated with LC2017 and with both products. Moreover, a specific response related to the cultivar was verified, but this apparently unique interaction between product, cultivar and pathogen remains to be further investigated.https://www.mdpi.com/2073-4395/12/5/996Botryosphaeria diebackinternal necrosis lengthgene expressioncuttingsTouriga NacionalCabernet Sauvignon |
| spellingShingle | Pedro Reis Vincenzo Mondello Inês Diniz Artur Alves Cecília Rego Florence Fontaine Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i> Agronomy Botryosphaeria dieback internal necrosis length gene expression cuttings Touriga Nacional Cabernet Sauvignon |
| title | Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i> |
| title_full | Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i> |
| title_fullStr | Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i> |
| title_full_unstemmed | Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i> |
| title_short | Effect of the Combined Treatments with LC2017 and <i>Trichoderma</i> <i>atroviride</i> Strain I-1237 on Disease Development and Defense Responses in Vines Infected by <i>Lasiodiplodia theobromae</i> |
| title_sort | effect of the combined treatments with lc2017 and i trichoderma i i atroviride i strain i 1237 on disease development and defense responses in vines infected by i lasiodiplodia theobromae i |
| topic | Botryosphaeria dieback internal necrosis length gene expression cuttings Touriga Nacional Cabernet Sauvignon |
| url | https://www.mdpi.com/2073-4395/12/5/996 |
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