Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation
<i>Codonopsis pilosula</i> is an important Chinese herbal medicine. However, fresh <i>C. pilosula</i> is prone to decay during storage due to microorganism infections, seriously affecting the medicinal value and even causing mycotoxin accumulation. Therefore, it is necessary...
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MDPI AG
2023-01-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/9/2/146 |
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| author | Bingyu Lv Xi Yang Huali Xue Mina Nan Yuan Zhang Zhiguang Liu Yang Bi Suqin Shang |
| author_facet | Bingyu Lv Xi Yang Huali Xue Mina Nan Yuan Zhang Zhiguang Liu Yang Bi Suqin Shang |
| author_sort | Bingyu Lv |
| collection | DOAJ |
| description | <i>Codonopsis pilosula</i> is an important Chinese herbal medicine. However, fresh <i>C. pilosula</i> is prone to decay during storage due to microorganism infections, seriously affecting the medicinal value and even causing mycotoxin accumulation. Therefore, it is necessary to study the pathogens present and develop efficient control strategies to mitigate their detrimental effects on the herbs during storage. In this study, fresh <i>C. pilosula</i> was collected from Min County in Gansu Province, China. The natural disease symptoms were observed during different storage stages, and the pathogens causing <i>C. pilosula</i> postharvest decay were isolated from the infected fresh <i>C. pilosula</i>. Morphological and molecular identification were performed, and pathogenicity was tested using Koch’s postulates. In addition, the control of ozone was examined against the isolates and mycotoxin accumulation. The results indicated that the naturally occurring symptom increased progressively with the extension of storage time. The mucor rot caused by <i>Mucor</i> was first observed on day 7, followed by root rot caused by <i>Fusarium</i> on day 14. Blue mold disease caused by <i>Penicillum expansum</i> was detected as the most serious postharvest disease on day 28. Pink rot disease caused by <i>Trichothecium roseum</i> was observed on day 56. Moreover, ozone treatment significantly decreased the development of postharvest disease and inhibited the accumulations of patulin, deoxynivalenol, 15-Acetyl-deoxynivalenol, and HT-2 toxin. |
| first_indexed | 2024-03-11T08:35:26Z |
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| id | doaj.art-232f4be3c53f4d3f818bcd25e96f0869 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T08:35:26Z |
| publishDate | 2023-01-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-232f4be3c53f4d3f818bcd25e96f08692023-11-16T21:29:44ZengMDPI AGJournal of Fungi2309-608X2023-01-019214610.3390/jof9020146Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin AccumulationBingyu Lv0Xi Yang1Huali Xue2Mina Nan3Yuan Zhang4Zhiguang Liu5Yang Bi6Suqin Shang7College of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Plant Protection, Gansu Agricultural University, Lanzhou 730070, China<i>Codonopsis pilosula</i> is an important Chinese herbal medicine. However, fresh <i>C. pilosula</i> is prone to decay during storage due to microorganism infections, seriously affecting the medicinal value and even causing mycotoxin accumulation. Therefore, it is necessary to study the pathogens present and develop efficient control strategies to mitigate their detrimental effects on the herbs during storage. In this study, fresh <i>C. pilosula</i> was collected from Min County in Gansu Province, China. The natural disease symptoms were observed during different storage stages, and the pathogens causing <i>C. pilosula</i> postharvest decay were isolated from the infected fresh <i>C. pilosula</i>. Morphological and molecular identification were performed, and pathogenicity was tested using Koch’s postulates. In addition, the control of ozone was examined against the isolates and mycotoxin accumulation. The results indicated that the naturally occurring symptom increased progressively with the extension of storage time. The mucor rot caused by <i>Mucor</i> was first observed on day 7, followed by root rot caused by <i>Fusarium</i> on day 14. Blue mold disease caused by <i>Penicillum expansum</i> was detected as the most serious postharvest disease on day 28. Pink rot disease caused by <i>Trichothecium roseum</i> was observed on day 56. Moreover, ozone treatment significantly decreased the development of postharvest disease and inhibited the accumulations of patulin, deoxynivalenol, 15-Acetyl-deoxynivalenol, and HT-2 toxin.https://www.mdpi.com/2309-608X/9/2/146<i>Codonopsis pilosula</i>postharvest diseasemorphological and molecular biology identificationozonemycotoxin accumulation |
| spellingShingle | Bingyu Lv Xi Yang Huali Xue Mina Nan Yuan Zhang Zhiguang Liu Yang Bi Suqin Shang Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation Journal of Fungi <i>Codonopsis pilosula</i> postharvest disease morphological and molecular biology identification ozone mycotoxin accumulation |
| title | Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation |
| title_full | Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation |
| title_fullStr | Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation |
| title_full_unstemmed | Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation |
| title_short | Isolation of Main Pathogens Causing Postharvest Disease in Fresh <i>Codonopsis pilosula</i> during Different Storage Stages and Ozone Control against Disease and Mycotoxin Accumulation |
| title_sort | isolation of main pathogens causing postharvest disease in fresh i codonopsis pilosula i during different storage stages and ozone control against disease and mycotoxin accumulation |
| topic | <i>Codonopsis pilosula</i> postharvest disease morphological and molecular biology identification ozone mycotoxin accumulation |
| url | https://www.mdpi.com/2309-608X/9/2/146 |
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