High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters
Autotrophic and heterotrophic bacterioplankton are essential to the biogeochemistry of tropical ecosystems. However, the processes that govern their dynamics are not well known. We provide here a high-frequency assessment of bacterial community dynamics and concurrent environmental factors in Red Se...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.780530/full |
| _version_ | 1811329132105039872 |
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| author | Mohd Ikram Ansari Mohd Ikram Ansari Maria LI. Calleja Maria LI. Calleja Luis Silva Miguel Viegas David Kamanda Ngugi David Kamanda Ngugi Tamara Megan Huete-Stauffer Xosé Anxelu G. Morán Xosé Anxelu G. Morán |
| author_facet | Mohd Ikram Ansari Mohd Ikram Ansari Maria LI. Calleja Maria LI. Calleja Luis Silva Miguel Viegas David Kamanda Ngugi David Kamanda Ngugi Tamara Megan Huete-Stauffer Xosé Anxelu G. Morán Xosé Anxelu G. Morán |
| author_sort | Mohd Ikram Ansari |
| collection | DOAJ |
| description | Autotrophic and heterotrophic bacterioplankton are essential to the biogeochemistry of tropical ecosystems. However, the processes that govern their dynamics are not well known. We provide here a high-frequency assessment of bacterial community dynamics and concurrent environmental factors in Red Sea coastal waters. Weekly sampling of surface samples during a full annual cycle at an enclosed station revealed high variability in ecological conditions, which reflected in changes of major bacterioplankton communities. Temperature varied between 23 and 34°C during the sampling period. Autotrophic (Synechococcus, 1.7–16.2 × 104 cells mL−1) and heterotrophic bacteria (1.6–4.3 × 105 cells mL−1) showed two maxima in abundance in spring and summer, while minima were found in winter and autumn. Heterotrophic cells with high nucleic acid content (HNA) peaked in July, but their contribution to the total cell counts (35–60%) did not show a clear seasonal pattern. Actively respiring cells (CTC+) contributed between 4 and 51% of the total number of heterotrophic bacteria, while live cells (with intact membrane) consistently accounted for over 90%. Sequenced 16S rRNA amplicons revealed a predominance of Proteobacteria in summer and autumn (>40%) and a smaller contribution in winter (21–24%), with members of the Alphaproteobacteria class dominating throughout the year. The contribution of the Flavobacteriaceae family was highest in winter (21%), while the Rhodobacteraceae contribution was lowest (6%). Temperature, chlorophyll-a, and dissolved organic carbon concentration were the environmental variables with the greatest effects on bacterial abundance and diversity patterns. |
| first_indexed | 2024-04-13T15:38:24Z |
| format | Article |
| id | doaj.art-233317fb9b8b404c86a349e85133e601 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T15:38:24Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-233317fb9b8b404c86a349e85133e6012022-12-22T02:41:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-03-011310.3389/fmicb.2022.780530780530High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal WatersMohd Ikram Ansari0Mohd Ikram Ansari1Maria LI. Calleja2Maria LI. Calleja3Luis Silva4Miguel Viegas5David Kamanda Ngugi6David Kamanda Ngugi7Tamara Megan Huete-Stauffer8Xosé Anxelu G. Morán9Xosé Anxelu G. Morán10Division of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDepartment of Biosciences, Integral University, Lucknow, IndiaDivision of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDepartment of Climate Geochemistry, Max Planck Institute for Chemistry (MPIC), Mainz, GermanyDivision of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDivision of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDivision of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDepartment of Microorganisms, Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Braunschweig, GermanyDivision of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDivision of Biological and Environmental Sciences and Engineering (BESE), Red Sea Research Center (RSRC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaCentro Oceanográfico de Gijón/Xixón (IEO, CSIC), Gijón/Xixón, SpainAutotrophic and heterotrophic bacterioplankton are essential to the biogeochemistry of tropical ecosystems. However, the processes that govern their dynamics are not well known. We provide here a high-frequency assessment of bacterial community dynamics and concurrent environmental factors in Red Sea coastal waters. Weekly sampling of surface samples during a full annual cycle at an enclosed station revealed high variability in ecological conditions, which reflected in changes of major bacterioplankton communities. Temperature varied between 23 and 34°C during the sampling period. Autotrophic (Synechococcus, 1.7–16.2 × 104 cells mL−1) and heterotrophic bacteria (1.6–4.3 × 105 cells mL−1) showed two maxima in abundance in spring and summer, while minima were found in winter and autumn. Heterotrophic cells with high nucleic acid content (HNA) peaked in July, but their contribution to the total cell counts (35–60%) did not show a clear seasonal pattern. Actively respiring cells (CTC+) contributed between 4 and 51% of the total number of heterotrophic bacteria, while live cells (with intact membrane) consistently accounted for over 90%. Sequenced 16S rRNA amplicons revealed a predominance of Proteobacteria in summer and autumn (>40%) and a smaller contribution in winter (21–24%), with members of the Alphaproteobacteria class dominating throughout the year. The contribution of the Flavobacteriaceae family was highest in winter (21%), while the Rhodobacteraceae contribution was lowest (6%). Temperature, chlorophyll-a, and dissolved organic carbon concentration were the environmental variables with the greatest effects on bacterial abundance and diversity patterns.https://www.frontiersin.org/articles/10.3389/fmicb.2022.780530/fullbacterioplanktonseasonalityflow cytometry16S rDNAnext-generation sequencingsingle-cell analysis |
| spellingShingle | Mohd Ikram Ansari Mohd Ikram Ansari Maria LI. Calleja Maria LI. Calleja Luis Silva Miguel Viegas David Kamanda Ngugi David Kamanda Ngugi Tamara Megan Huete-Stauffer Xosé Anxelu G. Morán Xosé Anxelu G. Morán High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters Frontiers in Microbiology bacterioplankton seasonality flow cytometry 16S rDNA next-generation sequencing single-cell analysis |
| title | High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters |
| title_full | High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters |
| title_fullStr | High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters |
| title_full_unstemmed | High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters |
| title_short | High-Frequency Variability of Bacterioplankton in Response to Environmental Drivers in Red Sea Coastal Waters |
| title_sort | high frequency variability of bacterioplankton in response to environmental drivers in red sea coastal waters |
| topic | bacterioplankton seasonality flow cytometry 16S rDNA next-generation sequencing single-cell analysis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.780530/full |
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