Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine
Background Reconstructed embryos from terminally differentiated somatic cells have revealed high levels of genomic methylation which results in inappropriate expression patterns of imprinted and non-imprinted genes. These aberrant expressions are probably responsible for different abnormalities duri...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2011-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | http://www.ijfs.ir/article_45069_0fbede76ae076f8b851bc96d3cbede2b.pdf |
| _version_ | 1831716146261262336 |
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| author | Farnoosh Jafarpour Sayed Morteza Hosseini Mahdi Hajian Mohsen Forouzanfar Parvaneh Abedi Laleh Hosseini Somaye Ostadhosseini Soghra Gholami Mohammad Hossein Nasr Esfahani |
| author_facet | Farnoosh Jafarpour Sayed Morteza Hosseini Mahdi Hajian Mohsen Forouzanfar Parvaneh Abedi Laleh Hosseini Somaye Ostadhosseini Soghra Gholami Mohammad Hossein Nasr Esfahani |
| author_sort | Farnoosh Jafarpour |
| collection | DOAJ |
| description | Background Reconstructed embryos from terminally differentiated somatic cells have revealed high levels of genomic methylation which results in inappropriate expression patterns of imprinted and non-imprinted genes. These aberrant expressions are probably responsible for different abnormalities during the development of clones. Improvement in cloning competency may be achieved through modification of epigenetic markers in donor cells. Materials and Methods Our objective was to determine if treatment of donor cells for 72 hours with 5-aza-2'-deoxycytidine (5-aza-dc; 0-0.3 μM), a DNA methyl transferase inhibitor, improved development and expression of Oct-4. Results In comparison with untreated cells, 0.01 and 0.08 μM 5-aza-dc treated cells insignificantly decreased the blastocyst rate (32.1% vs. 28.6% and 27.2%, respectively) while it was significant for 0.3 μM treated cells (6.5%). Embryo quality as measured by the total cell number (TCN) decreased in a dose-related fashion, which was significant at 0.08 and 0.3 μM 5-aza-dc treated cells when compared with 0 and 0.01 μM 5-aza-dc treated cells. Although reconstructed embryos from 0.08 and 0.3 μM 5-aza-dc treated cells showed lower levels of DNA methylation and histone H3 acetylation, development to blastocyst stage was decreased. The epigenetic markers of embryos cloned from 0.01 μM 5-aza-dc remained unchanged. Conclusion These results show that 5-aza-dc is not a suitable choice for modifying nuclear reprogramming. Finally, it was concluded that the wide genomic hypomethylation induced by 5-aza-dc deleteriously impacts the developmental competency of cloned embryos. |
| first_indexed | 2024-12-21T00:33:16Z |
| format | Article |
| id | doaj.art-233355ba492746a291ac768a389aec8e |
| institution | Directory Open Access Journal |
| issn | 2008-076X 2008-0778 |
| language | English |
| last_indexed | 2024-12-21T00:33:16Z |
| publishDate | 2011-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | International Journal of Fertility and Sterility |
| spelling | doaj.art-233355ba492746a291ac768a389aec8e2022-12-21T19:21:50ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782011-01-014414815545069Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidineFarnoosh Jafarpour0Sayed Morteza Hosseini1Mahdi Hajian2Mohsen Forouzanfar3Parvaneh Abedi4Laleh Hosseini5Somaye Ostadhosseini6Soghra Gholami7Mohammad Hossein Nasr Esfahani8Department of Anatomy and Embryology, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranSchool of Sciences, Islamic Azad University, Marvdasht Branch, Marvdasht, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranDepartment of Anatomy and Embryology, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Reproduction and Development, Reproductive Biomedicine Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan, IranBackground Reconstructed embryos from terminally differentiated somatic cells have revealed high levels of genomic methylation which results in inappropriate expression patterns of imprinted and non-imprinted genes. These aberrant expressions are probably responsible for different abnormalities during the development of clones. Improvement in cloning competency may be achieved through modification of epigenetic markers in donor cells. Materials and Methods Our objective was to determine if treatment of donor cells for 72 hours with 5-aza-2'-deoxycytidine (5-aza-dc; 0-0.3 μM), a DNA methyl transferase inhibitor, improved development and expression of Oct-4. Results In comparison with untreated cells, 0.01 and 0.08 μM 5-aza-dc treated cells insignificantly decreased the blastocyst rate (32.1% vs. 28.6% and 27.2%, respectively) while it was significant for 0.3 μM treated cells (6.5%). Embryo quality as measured by the total cell number (TCN) decreased in a dose-related fashion, which was significant at 0.08 and 0.3 μM 5-aza-dc treated cells when compared with 0 and 0.01 μM 5-aza-dc treated cells. Although reconstructed embryos from 0.08 and 0.3 μM 5-aza-dc treated cells showed lower levels of DNA methylation and histone H3 acetylation, development to blastocyst stage was decreased. The epigenetic markers of embryos cloned from 0.01 μM 5-aza-dc remained unchanged. Conclusion These results show that 5-aza-dc is not a suitable choice for modifying nuclear reprogramming. Finally, it was concluded that the wide genomic hypomethylation induced by 5-aza-dc deleteriously impacts the developmental competency of cloned embryos.http://www.ijfs.ir/article_45069_0fbede76ae076f8b851bc96d3cbede2b.pdfepigenesisnuclear reprogrammingnuclear transfer |
| spellingShingle | Farnoosh Jafarpour Sayed Morteza Hosseini Mahdi Hajian Mohsen Forouzanfar Parvaneh Abedi Laleh Hosseini Somaye Ostadhosseini Soghra Gholami Mohammad Hossein Nasr Esfahani Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine International Journal of Fertility and Sterility epigenesis nuclear reprogramming nuclear transfer |
| title | Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine |
| title_full | Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine |
| title_fullStr | Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine |
| title_full_unstemmed | Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine |
| title_short | Developmental Competence and Pluripotency Gene Expression of Cattle Cloned Embryos Derived from Donor Cells Treated with 5-aza-2'-deoxycytidine |
| title_sort | developmental competence and pluripotency gene expression of cattle cloned embryos derived from donor cells treated with 5 aza 2 deoxycytidine |
| topic | epigenesis nuclear reprogramming nuclear transfer |
| url | http://www.ijfs.ir/article_45069_0fbede76ae076f8b851bc96d3cbede2b.pdf |
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