Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts
<i>Clinacanthus nutans</i> is known to be an anticancer and antiviral agent, and <i>Strobilanthes crispus</i> has proven to be an antidiuretic and antidiabetic agent. However, there is a high possibility that these plants possess multiple beneficial properties, such as antimi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/5/1722 |
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| author | Weng Kit Ban Isabel Lim Fong Heng Yen Khong Joyce Hui Yie Phung |
| author_facet | Weng Kit Ban Isabel Lim Fong Heng Yen Khong Joyce Hui Yie Phung |
| author_sort | Weng Kit Ban |
| collection | DOAJ |
| description | <i>Clinacanthus nutans</i> is known to be an anticancer and antiviral agent, and <i>Strobilanthes crispus</i> has proven to be an antidiuretic and antidiabetic agent. However, there is a high possibility that these plants possess multiple beneficial properties, such as antimicrobial and wound healing properties. This study aims to assess the wound healing, antioxidant, and antimicrobial properties of <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i>. The <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i> leaves were dried, ground, and extracted with ethanol, acetone, and chloroform through cold maceration. In a modified scratch assay with co-incubation of skin fibroblast and Methicillin-resistant <i>Staphylococcus aureus</i>, <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i> extracts were assessed for their wound healing potential, and the antimicrobial activities of <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i> extracts were performed on a panel of Gram-positive and Gram-negative bacteria on Mueller–Hinton agar based on a disc diffusion assay. To assess for antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and total flavonoid assays were conducted. In the modified scratch assay, <i>Clinacanthus nutans</i> extracts aided in the wound healing activity while in the presence of MRSA, and <i>Strobilanthes crispus</i> extracts were superior in antimicrobial and wound healing activities. In addition, <i>Strobilanthes crispus</i> extracts were superior to <i>Clinacanthus nutans</i> extracts against <i>Pseudomonas aeruginosa</i> on Mueller–Hinton agar. Acetone-extracted <i>Clinacanthus nutans</i> contained the highest level of antioxidant in comparison with other <i>Clinacanthus nutans</i> extracts. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T20:28:51Z |
| publishDate | 2022-03-01 |
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| spelling | doaj.art-23364a7aa2b94e25b45fae09cfa966b02023-11-23T23:28:46ZengMDPI AGMolecules1420-30492022-03-01275172210.3390/molecules27051722Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> ExtractsWeng Kit Ban0Isabel Lim Fong1Heng Yen Khong2Joyce Hui Yie Phung3Department of Paraclinical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan 94300, MalaysiaDepartment of Paraclinical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan 94300, MalaysiaSchool of Chemistry and Environment, Faculty of Applied Sciences, Universiti Teknologi MARA, Sarawak Branch, Kota Samarahan 94300, MalaysiaDepartment of Paraclinical Sciences, Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Kota Samarahan 94300, Malaysia<i>Clinacanthus nutans</i> is known to be an anticancer and antiviral agent, and <i>Strobilanthes crispus</i> has proven to be an antidiuretic and antidiabetic agent. However, there is a high possibility that these plants possess multiple beneficial properties, such as antimicrobial and wound healing properties. This study aims to assess the wound healing, antioxidant, and antimicrobial properties of <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i>. The <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i> leaves were dried, ground, and extracted with ethanol, acetone, and chloroform through cold maceration. In a modified scratch assay with co-incubation of skin fibroblast and Methicillin-resistant <i>Staphylococcus aureus</i>, <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i> extracts were assessed for their wound healing potential, and the antimicrobial activities of <i>Clinacanthus nutans</i> and <i>Strobilanthes crispus</i> extracts were performed on a panel of Gram-positive and Gram-negative bacteria on Mueller–Hinton agar based on a disc diffusion assay. To assess for antioxidant potential, 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic and total flavonoid assays were conducted. In the modified scratch assay, <i>Clinacanthus nutans</i> extracts aided in the wound healing activity while in the presence of MRSA, and <i>Strobilanthes crispus</i> extracts were superior in antimicrobial and wound healing activities. In addition, <i>Strobilanthes crispus</i> extracts were superior to <i>Clinacanthus nutans</i> extracts against <i>Pseudomonas aeruginosa</i> on Mueller–Hinton agar. Acetone-extracted <i>Clinacanthus nutans</i> contained the highest level of antioxidant in comparison with other <i>Clinacanthus nutans</i> extracts.https://www.mdpi.com/1420-3049/27/5/1722<i>Clinacanthus nutans</i><i>Strobilanthes crispus</i>wound healingmethicillin-resistant <i>Staphylococcus aureus</i><i>Pseudomonas aeruginosa</i>scratch assay |
| spellingShingle | Weng Kit Ban Isabel Lim Fong Heng Yen Khong Joyce Hui Yie Phung Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts Molecules <i>Clinacanthus nutans</i> <i>Strobilanthes crispus</i> wound healing methicillin-resistant <i>Staphylococcus aureus</i> <i>Pseudomonas aeruginosa</i> scratch assay |
| title | Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts |
| title_full | Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts |
| title_fullStr | Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts |
| title_full_unstemmed | Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts |
| title_short | Wound Healing, Antimicrobial and Antioxidant Properties of <i>Clinacanthus nutans</i> (Burm.f.) <i>Lindau</i> and <i>Strobilanthes crispus</i> (L.) <i>Blume</i> Extracts |
| title_sort | wound healing antimicrobial and antioxidant properties of i clinacanthus nutans i burm f i lindau i and i strobilanthes crispus i l i blume i extracts |
| topic | <i>Clinacanthus nutans</i> <i>Strobilanthes crispus</i> wound healing methicillin-resistant <i>Staphylococcus aureus</i> <i>Pseudomonas aeruginosa</i> scratch assay |
| url | https://www.mdpi.com/1420-3049/27/5/1722 |
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