Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism
Objective: Under the guidance of model organism Caenorhabditis elegans with fine olfactory system, small molecular metabolites sensitive to high dose radiation were screened as biomarkers of acute radiation-induced injury, and their metabolic pathways were elucidated by enrichment. Methods: Rats wer...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-09-01
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| Series: | Radiation Medicine and Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666555721000435 |
| _version_ | 1797723602984894464 |
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| author | Xin Wu Tong Zhu Hang Li Xin He Sai-jun Fan |
| author_facet | Xin Wu Tong Zhu Hang Li Xin He Sai-jun Fan |
| author_sort | Xin Wu |
| collection | DOAJ |
| description | Objective: Under the guidance of model organism Caenorhabditis elegans with fine olfactory system, small molecular metabolites sensitive to high dose radiation were screened as biomarkers of acute radiation-induced injury, and their metabolic pathways were elucidated by enrichment. Methods: Rats were irradiated with 12 Gy γ-rays to establish an acute radiation injury model, and their urine was fingerprinted using UPLC-Q/TOF-MS. Further, under the guidance of Caenorhabditis elegans as olfactory-sensitive model organism, the key differential metabolites in urine were found as biomarkers of radiation-induced injury. Results: After rats were irradiated, the radiation injury urine showed a difference from control (sham-irradiated) urine, which could be distinguished by Caenorhabditis elegans. Based on metabolomics analysis, a total of 21 key differential metabolites with P value < 0.05 and fold change either >2 or <0.5 were identified, which can be used as sensitive and reliable biomarkers of radiation-induced injury. The pathways were further enriched, and it was found that disorders of five metabolic pathways, including citric acid cycle and amino acid metabolism, play an important role in radiation-induced injury. Conclusions: Due to radiation injury, the metabolites in urine will change significantly. The study on biomarkers guided by model organism Caenorhabditis elegans provides a new perspective to explain the details of metabolic disorders, and also provides experimental basis for the development of new biological dosimeters. |
| first_indexed | 2024-03-12T10:05:06Z |
| format | Article |
| id | doaj.art-233696fe9e8c4bf39df3d47805db8733 |
| institution | Directory Open Access Journal |
| issn | 2666-5557 |
| language | English |
| last_indexed | 2024-03-12T10:05:06Z |
| publishDate | 2021-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Radiation Medicine and Protection |
| spelling | doaj.art-233696fe9e8c4bf39df3d47805db87332023-09-02T11:19:07ZengElsevierRadiation Medicine and Protection2666-55572021-09-01238994Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organismXin Wu0Tong Zhu1Hang Li2Xin He3Sai-jun Fan4Corresponding author. Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Tianjin, 300192, China.; Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, ChinaTianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, ChinaTianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, ChinaTianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, ChinaCorresponding author.; Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, ChinaObjective: Under the guidance of model organism Caenorhabditis elegans with fine olfactory system, small molecular metabolites sensitive to high dose radiation were screened as biomarkers of acute radiation-induced injury, and their metabolic pathways were elucidated by enrichment. Methods: Rats were irradiated with 12 Gy γ-rays to establish an acute radiation injury model, and their urine was fingerprinted using UPLC-Q/TOF-MS. Further, under the guidance of Caenorhabditis elegans as olfactory-sensitive model organism, the key differential metabolites in urine were found as biomarkers of radiation-induced injury. Results: After rats were irradiated, the radiation injury urine showed a difference from control (sham-irradiated) urine, which could be distinguished by Caenorhabditis elegans. Based on metabolomics analysis, a total of 21 key differential metabolites with P value < 0.05 and fold change either >2 or <0.5 were identified, which can be used as sensitive and reliable biomarkers of radiation-induced injury. The pathways were further enriched, and it was found that disorders of five metabolic pathways, including citric acid cycle and amino acid metabolism, play an important role in radiation-induced injury. Conclusions: Due to radiation injury, the metabolites in urine will change significantly. The study on biomarkers guided by model organism Caenorhabditis elegans provides a new perspective to explain the details of metabolic disorders, and also provides experimental basis for the development of new biological dosimeters.http://www.sciencedirect.com/science/article/pii/S2666555721000435Radiation-induced injuryModel organismUrineMetabolomicsBiomarkers |
| spellingShingle | Xin Wu Tong Zhu Hang Li Xin He Sai-jun Fan Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism Radiation Medicine and Protection Radiation-induced injury Model organism Urine Metabolomics Biomarkers |
| title | Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism |
| title_full | Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism |
| title_fullStr | Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism |
| title_full_unstemmed | Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism |
| title_short | Study on urine biomarkers of radiation-induced injury guided by Caenorhabditis elegans as a model organism |
| title_sort | study on urine biomarkers of radiation induced injury guided by caenorhabditis elegans as a model organism |
| topic | Radiation-induced injury Model organism Urine Metabolomics Biomarkers |
| url | http://www.sciencedirect.com/science/article/pii/S2666555721000435 |
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