Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha
Background: Human papillomavirus (HPV) vaccination is one of the crucial national vaccination programs aimed at reducing the prevalence of the diseases associated with HPV infections, which continue to pose a global health concern. However, a significant disparity exists in the distribution of HPV v...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-03-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X23015135 |
| _version_ | 1797259999085330432 |
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| author | Sheila Chairunnisa Apon Zaenal Mustopa Budiman Bela Moh Egy Rahman Firdaus Shasmita Irawan Rosyida Khusniatul Arifah Herman Irawan Maritsa Nurfatwa Rifqiyah Nur Umami Nurlaili Ekawati Ai Hertati Nurhasni Hasan |
| author_facet | Sheila Chairunnisa Apon Zaenal Mustopa Budiman Bela Moh Egy Rahman Firdaus Shasmita Irawan Rosyida Khusniatul Arifah Herman Irawan Maritsa Nurfatwa Rifqiyah Nur Umami Nurlaili Ekawati Ai Hertati Nurhasni Hasan |
| author_sort | Sheila Chairunnisa |
| collection | DOAJ |
| description | Background: Human papillomavirus (HPV) vaccination is one of the crucial national vaccination programs aimed at reducing the prevalence of the diseases associated with HPV infections, which continue to pose a global health concern. However, a significant disparity exists in the distribution of HPV vaccine, particularly in low-middle income countries where the cost of HPV vaccine becomes a major obstacle. Thus, it is essential to ensure the availability of an economically feasible HPV vaccine, necessitating immediate efforts to enhance the cost-effectiveness of vaccine production. This study aimed to develop an efficient production system for the recombinant HPV type 52 L1 protein as HPV vaccine material using methylotrophic yeast Hansenula polymorpha expression system. Results: This study presents an in-depth examination of the expression and scale-up production of HPV type 52 L1 protein using DASGIP® parallel bioreactor system. The pHIPX4 plasmid, which is regulated by the MOX promoter, generates stable clones that express the target protein. Cultivation employing the synthetic medium SYN6(10) with controlled parameters (e.g. temperature, pH, feeding strategy, and aeration) produces 0.15 µg/mL of HPV type 52 L1 protein, suggesting a possibility for scaling up to a higher production level. Conclusion: The scale-up production of HPV type 52 L1 protein using Hansenula polymorpha expression system described in this study provides an opportunity for an economical manufacturing platform for the development of the HPV vaccine. |
| first_indexed | 2024-04-24T23:18:21Z |
| format | Article |
| id | doaj.art-23385ec04eaa4adf92958888e5a7d05a |
| institution | Directory Open Access Journal |
| issn | 1687-157X |
| language | English |
| last_indexed | 2024-04-24T23:18:21Z |
| publishDate | 2024-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-23385ec04eaa4adf92958888e5a7d05a2024-03-17T07:53:11ZengSpringerOpenJournal of Genetic Engineering and Biotechnology1687-157X2024-03-01221100342Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorphaSheila Chairunnisa0Apon Zaenal Mustopa1Budiman Bela2Moh Egy Rahman Firdaus3Shasmita Irawan4Rosyida Khusniatul Arifah5Herman Irawan6Maritsa Nurfatwa7Rifqiyah Nur Umami8Nurlaili Ekawati9Ai Hertati10Nurhasni Hasan11Master's Programme in Biomedical Sciences, Faculty of Medicine Universitas Indonesia, Jakarta 10430, Indonesia; Research Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, Indonesia; Corresponding author.Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaResearch Center for Genetic Engineering, National Research and Innovation Agency (BRIN), Bogor 16911, IndonesiaFaculty of Pharmacy, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km 10, Makassar 90245, IndonesiaBackground: Human papillomavirus (HPV) vaccination is one of the crucial national vaccination programs aimed at reducing the prevalence of the diseases associated with HPV infections, which continue to pose a global health concern. However, a significant disparity exists in the distribution of HPV vaccine, particularly in low-middle income countries where the cost of HPV vaccine becomes a major obstacle. Thus, it is essential to ensure the availability of an economically feasible HPV vaccine, necessitating immediate efforts to enhance the cost-effectiveness of vaccine production. This study aimed to develop an efficient production system for the recombinant HPV type 52 L1 protein as HPV vaccine material using methylotrophic yeast Hansenula polymorpha expression system. Results: This study presents an in-depth examination of the expression and scale-up production of HPV type 52 L1 protein using DASGIP® parallel bioreactor system. The pHIPX4 plasmid, which is regulated by the MOX promoter, generates stable clones that express the target protein. Cultivation employing the synthetic medium SYN6(10) with controlled parameters (e.g. temperature, pH, feeding strategy, and aeration) produces 0.15 µg/mL of HPV type 52 L1 protein, suggesting a possibility for scaling up to a higher production level. Conclusion: The scale-up production of HPV type 52 L1 protein using Hansenula polymorpha expression system described in this study provides an opportunity for an economical manufacturing platform for the development of the HPV vaccine.http://www.sciencedirect.com/science/article/pii/S1687157X23015135HPV type 52 L1 proteinHansenula polymorphaSynthetic mediumDASGIP® parallel bioreactor system |
| spellingShingle | Sheila Chairunnisa Apon Zaenal Mustopa Budiman Bela Moh Egy Rahman Firdaus Shasmita Irawan Rosyida Khusniatul Arifah Herman Irawan Maritsa Nurfatwa Rifqiyah Nur Umami Nurlaili Ekawati Ai Hertati Nurhasni Hasan Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha Journal of Genetic Engineering and Biotechnology HPV type 52 L1 protein Hansenula polymorpha Synthetic medium DASGIP® parallel bioreactor system |
| title | Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha |
| title_full | Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha |
| title_fullStr | Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha |
| title_full_unstemmed | Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha |
| title_short | Expression and scale-up production of recombinant human papillomavirus type 52 L1 protein in methylotrophic yeast Hansenula polymorpha |
| title_sort | expression and scale up production of recombinant human papillomavirus type 52 l1 protein in methylotrophic yeast hansenula polymorpha |
| topic | HPV type 52 L1 protein Hansenula polymorpha Synthetic medium DASGIP® parallel bioreactor system |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X23015135 |
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