T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved]
Background: A potential explanation for the fact that the high rate of infection of SARS-CoV-2 in South Africa did not translate into high rates of severe illness and death may be the presence of cross-reactive immunity induced by common cold coronaviruses (CCoV). Methods: We used SARS-CoV-2 peptide...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2022-07-01
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| Series: | Gates Open Research |
| Subjects: | |
| Online Access: | https://gatesopenresearch.org/articles/5-150/v2 |
| _version_ | 1811309163298422784 |
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| author | Marta C. Nunes Gaurav Kwatra Michael J. Johnson Shabir A. Madhi Adriana Weinberg |
| author_facet | Marta C. Nunes Gaurav Kwatra Michael J. Johnson Shabir A. Madhi Adriana Weinberg |
| author_sort | Marta C. Nunes |
| collection | DOAJ |
| description | Background: A potential explanation for the fact that the high rate of infection of SARS-CoV-2 in South Africa did not translate into high rates of severe illness and death may be the presence of cross-reactive immunity induced by common cold coronaviruses (CCoV). Methods: We used SARS-CoV-2 peptide pools and whole virus antigen to stimulate peripheral blood mononuclear cells collected pre-2020 from South African women. Dual-colour FluoroSpot assay was used to measure interferon gamma (IFNγ) and interleukin 2 (IL2) production. Results: Among the 97 study participants, IFNγ responses were observed in 29.9% of the women and IL2 among 39.2%. Overall, 51.6% of women demonstrated response to at least one stimulant. Conclusion: We demonstrate the presence of cross-reactive immunity to SARS-CoV-2, which might have been induced by past exposure to CCoV. |
| first_indexed | 2024-04-13T09:36:20Z |
| format | Article |
| id | doaj.art-233b2ca220d4475297eed9d4589470d6 |
| institution | Directory Open Access Journal |
| issn | 2572-4754 |
| language | English |
| last_indexed | 2024-04-13T09:36:20Z |
| publishDate | 2022-07-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | Gates Open Research |
| spelling | doaj.art-233b2ca220d4475297eed9d4589470d62022-12-22T02:52:04ZengF1000 Research LtdGates Open Research2572-47542022-07-01515006T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved]Marta C. Nunes0https://orcid.org/0000-0003-3788-878XGaurav Kwatra1Michael J. Johnson2Shabir A. Madhi3Adriana Weinberg4South African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South AfricaSouth African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South AfricaDepartment of Pediatrics, Medicine and Pathology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USASouth African Medical Research Council, Vaccines and Infectious Diseases Analytics Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South AfricaDepartment of Pediatrics, Medicine and Pathology, University of Colorado, Anschutz Medical Campus, Aurora, Colorado, USABackground: A potential explanation for the fact that the high rate of infection of SARS-CoV-2 in South Africa did not translate into high rates of severe illness and death may be the presence of cross-reactive immunity induced by common cold coronaviruses (CCoV). Methods: We used SARS-CoV-2 peptide pools and whole virus antigen to stimulate peripheral blood mononuclear cells collected pre-2020 from South African women. Dual-colour FluoroSpot assay was used to measure interferon gamma (IFNγ) and interleukin 2 (IL2) production. Results: Among the 97 study participants, IFNγ responses were observed in 29.9% of the women and IL2 among 39.2%. Overall, 51.6% of women demonstrated response to at least one stimulant. Conclusion: We demonstrate the presence of cross-reactive immunity to SARS-CoV-2, which might have been induced by past exposure to CCoV.https://gatesopenresearch.org/articles/5-150/v2cell mediated immunity SARS-CoV-2 interferon gamma interleukin 2eng |
| spellingShingle | Marta C. Nunes Gaurav Kwatra Michael J. Johnson Shabir A. Madhi Adriana Weinberg T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved] Gates Open Research cell mediated immunity SARS-CoV-2 interferon gamma interleukin 2 eng |
| title | T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved] |
| title_full | T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved] |
| title_fullStr | T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved] |
| title_full_unstemmed | T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved] |
| title_short | T-cell responses to SARS-CoV-2 in unexposed South African women [version 2; peer review: 2 approved] |
| title_sort | t cell responses to sars cov 2 in unexposed south african women version 2 peer review 2 approved |
| topic | cell mediated immunity SARS-CoV-2 interferon gamma interleukin 2 eng |
| url | https://gatesopenresearch.org/articles/5-150/v2 |
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