Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery
Butyric acid, a four-carbon fatty acid, is an important industrial chemical and feedstock. To produce this chemical, a control fermentation was run with a 126.5 g.L<sup>−1</sup> glucose concentration in the feed medium. In this medium, the strain produced 44.8 g.L<sup>−1</sup>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/3/142 |
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| author | Nasib Qureshi Richard D. Ashby Nancy N. Nichols Ronald Hector |
| author_facet | Nasib Qureshi Richard D. Ashby Nancy N. Nichols Ronald Hector |
| author_sort | Nasib Qureshi |
| collection | DOAJ |
| description | Butyric acid, a four-carbon fatty acid, is an important industrial chemical and feedstock. To produce this chemical, a control fermentation was run with a 126.5 g.L<sup>−1</sup> glucose concentration in the feed medium. In this medium, the strain produced 44.8 g.L<sup>−1</sup> total acid with a productivity of 0.23 g.L<sup>−1</sup>h<sup>−1</sup> and a yield of 0.41 g.g<sup>−1</sup>. The strain (<i>Clostridium tyrobutyricum</i> ATCC 25755) was also able to utilize glucose and xylose simultaneously with similar fermentation performance. The culture was also used to produce butyric acid from wheat straw hydrolysate (WSH) employing a hot water pretreatment. In a batch system, the strain resulted in a productivity and yield of 0.27 g.L<sup>−1</sup>h<sup>−1</sup> and 0.44 g.g<sup>−1</sup>, respectively, which was an improvement over the use of glucose or xylose alone or mixtures of both. To improve reactor productivity, a membrane cell recycle bioreactor was used which resulted in a productivity of 1.89 g.L<sup>−1</sup>h<sup>−1</sup>. This productivity was 822% of that achieved in the glucose or xylose batch fermentation. Furthermore, a butyric acid recovery method was developed using XAD-4 adsorbent resin. In this system, up to 206.1 g.L<sup>−1</sup> of butyric acid was used in the feed and, as a result of the quick adsorption, the residual butyric acid concentration was 29.5 g.L<sup>−1</sup>. In this experiment, the rate of acid removal of 1059.4 g.L<sup>−1</sup>h<sup>−1</sup> was achieved. |
| first_indexed | 2024-04-24T18:17:44Z |
| format | Article |
| id | doaj.art-2340f54842d84409927c0aba18319a0f |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-04-24T18:17:44Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-2340f54842d84409927c0aba18319a0f2024-03-27T13:37:57ZengMDPI AGFermentation2311-56372024-03-0110314210.3390/fermentation10030142Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product RecoveryNasib Qureshi0Richard D. Ashby1Nancy N. Nichols2Ronald Hector3United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 N. University Street, Peoria, IL 61604, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 N. University Street, Peoria, IL 61604, USAUnited States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Bioenergy Research Unit, 1815 N. University Street, Peoria, IL 61604, USAButyric acid, a four-carbon fatty acid, is an important industrial chemical and feedstock. To produce this chemical, a control fermentation was run with a 126.5 g.L<sup>−1</sup> glucose concentration in the feed medium. In this medium, the strain produced 44.8 g.L<sup>−1</sup> total acid with a productivity of 0.23 g.L<sup>−1</sup>h<sup>−1</sup> and a yield of 0.41 g.g<sup>−1</sup>. The strain (<i>Clostridium tyrobutyricum</i> ATCC 25755) was also able to utilize glucose and xylose simultaneously with similar fermentation performance. The culture was also used to produce butyric acid from wheat straw hydrolysate (WSH) employing a hot water pretreatment. In a batch system, the strain resulted in a productivity and yield of 0.27 g.L<sup>−1</sup>h<sup>−1</sup> and 0.44 g.g<sup>−1</sup>, respectively, which was an improvement over the use of glucose or xylose alone or mixtures of both. To improve reactor productivity, a membrane cell recycle bioreactor was used which resulted in a productivity of 1.89 g.L<sup>−1</sup>h<sup>−1</sup>. This productivity was 822% of that achieved in the glucose or xylose batch fermentation. Furthermore, a butyric acid recovery method was developed using XAD-4 adsorbent resin. In this system, up to 206.1 g.L<sup>−1</sup> of butyric acid was used in the feed and, as a result of the quick adsorption, the residual butyric acid concentration was 29.5 g.L<sup>−1</sup>. In this experiment, the rate of acid removal of 1059.4 g.L<sup>−1</sup>h<sup>−1</sup> was achieved.https://www.mdpi.com/2311-5637/10/3/142butyric acid fermentationcellulosic sugarswheat strawbiomassmembrane reactor<i>Clostridium tyrobutyricum</i> |
| spellingShingle | Nasib Qureshi Richard D. Ashby Nancy N. Nichols Ronald Hector Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery Fermentation butyric acid fermentation cellulosic sugars wheat straw biomass membrane reactor <i>Clostridium tyrobutyricum</i> |
| title | Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery |
| title_full | Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery |
| title_fullStr | Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery |
| title_full_unstemmed | Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery |
| title_short | Novel Technologies for Butyric Acid Fermentation: Use of Cellulosic Biomass, Rapid Bioreactor, and Efficient Product Recovery |
| title_sort | novel technologies for butyric acid fermentation use of cellulosic biomass rapid bioreactor and efficient product recovery |
| topic | butyric acid fermentation cellulosic sugars wheat straw biomass membrane reactor <i>Clostridium tyrobutyricum</i> |
| url | https://www.mdpi.com/2311-5637/10/3/142 |
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