First Detection of Nosema ceranae Infecting Apis mellifera in India
Apis mellifera colonies were sampled for over twelve months to study the Nosema infection in different apiaries of Himachal Pradesh, India. We found that the infection incidence was highest in the winter season (48.40 %) followed by autumn and rainy months. The infection was diagnosed conventionally...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2019-06-01
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| Series: | Journal of Apicultural Science |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/jas-2019-0002 |
| _version_ | 1818587845080645632 |
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| author | Sharma Divya Katna Sapna Sharma Reena Rana Bachittar S. Sharma Harish K. Bhardwaj Vinay Chauhan Avinash |
| author_facet | Sharma Divya Katna Sapna Sharma Reena Rana Bachittar S. Sharma Harish K. Bhardwaj Vinay Chauhan Avinash |
| author_sort | Sharma Divya |
| collection | DOAJ |
| description | Apis mellifera colonies were sampled for over twelve months to study the Nosema infection in different apiaries of Himachal Pradesh, India. We found that the infection incidence was highest in the winter season (48.40 %) followed by autumn and rainy months. The infection was diagnosed conventionally through the microscopic analysis detection of Nosema species spores. However, with the recent findings that both N. ceranae and N. apis affect western honey bees, more sensitive and species specific molecular techniques are required that reliably differentiate between species of microsporidia. Therefore, molecular studies were conducted to precisely differentiate between Nosema species infecting honeybee colonies in India. Species specific multiplex PCR amplification using previously reported primers derived from 16S rRNA fragments were performed in a single reaction mixture. A single diagnostic band size of only 143 bp for N. ceranae was observed in the agarose gel. Furthermore, a diagnostic band size of 252 bp was observed with primer NoS, thus reconfirming the presence of N. ceranae. This study appears to be the first to report the detect of N. ceranae species from India in samples of A. mellifera. |
| first_indexed | 2024-12-16T09:15:19Z |
| format | Article |
| id | doaj.art-23432f4932de44c9a13bc1c9b4fb1d29 |
| institution | Directory Open Access Journal |
| issn | 2299-4831 |
| language | English |
| last_indexed | 2024-12-16T09:15:19Z |
| publishDate | 2019-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Apicultural Science |
| spelling | doaj.art-23432f4932de44c9a13bc1c9b4fb1d292022-12-21T22:36:53ZengSciendoJournal of Apicultural Science2299-48312019-06-0163116517010.2478/jas-2019-0002jas-2019-0002First Detection of Nosema ceranae Infecting Apis mellifera in IndiaSharma Divya0Katna Sapna1Sharma Reena2Rana Bachittar S.3Sharma Harish K.4Bhardwaj Vinay5Chauhan Avinash6Dr. Y S Parmar University of Horticulture and Foresrty, Solan, IndiaDr. Y S Parmar University of Horticulture and Foresrty, Solan, IndiaCentral Potato Research Institute, Shimla, IndiaDr. Y S Parmar University of Horticulture and Foresrty, Solan, IndiaDr. Y S Parmar University of Horticulture and Foresrty, Solan, IndiaCentral Potato Research Institute, Shimla, IndiaDr. Y S Parmar University of Horticulture and Foresrty, Solan, IndiaApis mellifera colonies were sampled for over twelve months to study the Nosema infection in different apiaries of Himachal Pradesh, India. We found that the infection incidence was highest in the winter season (48.40 %) followed by autumn and rainy months. The infection was diagnosed conventionally through the microscopic analysis detection of Nosema species spores. However, with the recent findings that both N. ceranae and N. apis affect western honey bees, more sensitive and species specific molecular techniques are required that reliably differentiate between species of microsporidia. Therefore, molecular studies were conducted to precisely differentiate between Nosema species infecting honeybee colonies in India. Species specific multiplex PCR amplification using previously reported primers derived from 16S rRNA fragments were performed in a single reaction mixture. A single diagnostic band size of only 143 bp for N. ceranae was observed in the agarose gel. Furthermore, a diagnostic band size of 252 bp was observed with primer NoS, thus reconfirming the presence of N. ceranae. This study appears to be the first to report the detect of N. ceranae species from India in samples of A. mellifera.https://doi.org/10.2478/jas-2019-0002apis melliferaindiamicroscopymultiplex pcrnosema ceranae |
| spellingShingle | Sharma Divya Katna Sapna Sharma Reena Rana Bachittar S. Sharma Harish K. Bhardwaj Vinay Chauhan Avinash First Detection of Nosema ceranae Infecting Apis mellifera in India Journal of Apicultural Science apis mellifera india microscopy multiplex pcr nosema ceranae |
| title | First Detection of Nosema ceranae Infecting Apis mellifera in India |
| title_full | First Detection of Nosema ceranae Infecting Apis mellifera in India |
| title_fullStr | First Detection of Nosema ceranae Infecting Apis mellifera in India |
| title_full_unstemmed | First Detection of Nosema ceranae Infecting Apis mellifera in India |
| title_short | First Detection of Nosema ceranae Infecting Apis mellifera in India |
| title_sort | first detection of nosema ceranae infecting apis mellifera in india |
| topic | apis mellifera india microscopy multiplex pcr nosema ceranae |
| url | https://doi.org/10.2478/jas-2019-0002 |
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