Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells
The eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) formulation with a ratio of 6:1 is a potent stimulator of the endothelial formation of nitric oxide (NO). The aim of the study was to investigate whether nanoencapsulation of EPA:DHA 6:1 followed by coating with gum increases its biologi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621005004 |
| _version_ | 1818700842226679808 |
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| author | L. Remila E. Belcastro N. Guenday-Tuereli S. Park U. Houngue T. Vandamme E. Tuereli P. Kerth C. Auger V. Schini-Kerth |
| author_facet | L. Remila E. Belcastro N. Guenday-Tuereli S. Park U. Houngue T. Vandamme E. Tuereli P. Kerth C. Auger V. Schini-Kerth |
| author_sort | L. Remila |
| collection | DOAJ |
| description | The eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) formulation with a ratio of 6:1 is a potent stimulator of the endothelial formation of nitric oxide (NO). The aim of the study was to investigate whether nanoencapsulation of EPA:DHA 6:1 followed by coating with gum increases its biological activity. Vascular reactivity was assessed using porcine coronary artery rings, the formation of NO in cultured endothelial cells (ECs) using DAF-FM and indirectly by platelet aggregation studies. Coated EPA:DHA 6:1 nanoparticles induced sustained relaxations of coronary artery rings that were greater in rings with than in those without endothelium, and more pronounced than with the native form. Treatment of ECs with coated EPA:DHA 6:1 nanoparticles caused greater and more sustained formation of NO and enhanced their anti-aggregatory effects. Thus, nanoencapsulation of EPA:DHA 6:1 is an attractive strategy to enhance the beneficial effect at the vascular endothelium. |
| first_indexed | 2024-12-17T15:11:22Z |
| format | Article |
| id | doaj.art-2347271eb122473e919adf113b841730 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-17T15:11:22Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-2347271eb122473e919adf113b8417302022-12-21T21:43:39ZengElsevierJournal of Functional Foods1756-46462021-12-0187104851Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cellsL. Remila0E. Belcastro1N. Guenday-Tuereli2S. Park3U. Houngue4T. Vandamme5E. Tuereli6P. Kerth7C. Auger8V. Schini-Kerth9University of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, FranceUniversity of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, FranceMyBiotech GmbH, Ueberherrn, GermanyUniversity of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, FranceUniversity of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, FranceUniversity of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, FranceMyBiotech GmbH, Ueberherrn, GermanyPreventor TBC GmbH, Pfungstadt, GermanyUniversity of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, FranceUniversity of Strasbourg, INSERM UMR 1260, Regenerative Nanomedicine, Strasbourg, France; Corresponding author at: INSERM UMR 1260, Faculty of Pharmacy, University of Strasbourg, 1, rue Eugène Boeckel, 67084 Strasbourg, France.The eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) formulation with a ratio of 6:1 is a potent stimulator of the endothelial formation of nitric oxide (NO). The aim of the study was to investigate whether nanoencapsulation of EPA:DHA 6:1 followed by coating with gum increases its biological activity. Vascular reactivity was assessed using porcine coronary artery rings, the formation of NO in cultured endothelial cells (ECs) using DAF-FM and indirectly by platelet aggregation studies. Coated EPA:DHA 6:1 nanoparticles induced sustained relaxations of coronary artery rings that were greater in rings with than in those without endothelium, and more pronounced than with the native form. Treatment of ECs with coated EPA:DHA 6:1 nanoparticles caused greater and more sustained formation of NO and enhanced their anti-aggregatory effects. Thus, nanoencapsulation of EPA:DHA 6:1 is an attractive strategy to enhance the beneficial effect at the vascular endothelium.http://www.sciencedirect.com/science/article/pii/S1756464621005004Omega-3NanoencapsulationEPA:DHA 6:1EndotheliumNitric oxide |
| spellingShingle | L. Remila E. Belcastro N. Guenday-Tuereli S. Park U. Houngue T. Vandamme E. Tuereli P. Kerth C. Auger V. Schini-Kerth Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells Journal of Functional Foods Omega-3 Nanoencapsulation EPA:DHA 6:1 Endothelium Nitric oxide |
| title | Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells |
| title_full | Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells |
| title_fullStr | Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells |
| title_full_unstemmed | Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells |
| title_short | Nanoencapsulation of the omega-3 EPA:DHA 6:1 formulation enhances and sustains NO-mediated endothelium-dependent relaxations in coronary artery rings and NO formation in endothelial cells |
| title_sort | nanoencapsulation of the omega 3 epa dha 6 1 formulation enhances and sustains no mediated endothelium dependent relaxations in coronary artery rings and no formation in endothelial cells |
| topic | Omega-3 Nanoencapsulation EPA:DHA 6:1 Endothelium Nitric oxide |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621005004 |
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