Measurement of cholesterol 7 alpha-hydroxylase activity with selected ion monitoring.

Measurement of cholesterol 7 alpha-hydroxylase activity with selected ion monitoring.

Formation of 7 alpha-hydroxycholesterol by rat liver microsomes was quantitated using a gas chromatograph-mass spectrometer operated in selected ion monitoring mode. This procedure measures the mass of 7 alpha-hydroxycholesterol formed and does not require addition of labeled cholesterol to monitor...

Full description

Bibliographic Details
Main Authors:	A Sanghvi, E Grassi, C Bartman, R Lester, M Galli Kienle, G Galli
Format:	Article
Language:	English
Published:	Elsevier 1981-05-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520373855

Similar Items

12 alpha-hydroxylase activity in human liver and its relation to cholesterol 7 alpha-hydroxylase activity.
by: K Einarsson, et al.
Published: (1992-11-01)

Transcriptional regulation of the human cholesterol 7 alpha-hydroxylase gene (CYP7A) in HepG2 cells
by: D P Wang, et al.
Published: (1996-09-01)

Hormonal regulation of the cholesterol 7 alpha-hydroxylase gene (CYP7)
by: M Crestani, et al.
Published: (1995-11-01)

Transcriptional activation of the cholesterol 7α-hydroxylase gene (CYP7A) by nuclear hormone receptors
by: Maurizio Crestani, et al.
Published: (1998-11-01)

On the saturation of the cholesterol 7 alpha-hydroxylase in human liver microsomes.
by: K Einarsson, et al.
Published: (1989-10-01)

Cholesterol 7 alpha-hydroxylase: evidence for transcriptional regulation by cholesterol or metabolic products of cholesterol in the rat
by: MP Jones, et al.
Published: (1993-06-01)

Expression and purification of human cholesterol 7 alpha-hydroxylase in Escherichia coli.
by: W G Karam, et al.
Published: (1994-07-01)

Effects of drugs and sterols on cholesterol 7 alpha-hydroxylase activity in rat liver microsomes
by: M A Schwartz, et al.
Published: (1983-01-01)

Regulation of bile acid synthesis. Measurement of cholesterol 7 alpha-hydroxylase activity in rat liver microsomal preparations in the absence of endogenous cholesterol
by: S Shefer, et al.
Published: (1981-03-01)

The pool of free cholesterol is not of major importance for regulation of the cholesterol 7 alpha-hydroxylase activity in rat liver microsomes.
by: K Einarsson, et al.
Published: (1987-03-01)

Cholesterol 7α-hydroxylase
by: N B Myant, et al.
Published: (1977-03-01)

On the possible use of the serum level of 7 alpha-hydroxycholesterol as a marker for increased activity of the cholesterol 7 alpha-hydroxylase in humans
by: I Björkhem, et al.
Published: (1987-08-01)

Regulation of rat liver microsomal cholesterol 7 alpha-hydroxylase: presence of a cytosolic activator.
by: C T Kwok, et al.
Published: (1981-05-01)

Effect of dietary cholesterol and taurocholate on cholesterol 7 alpha-hydroxylase and hepatic LDL receptors in inbred mice
by: S Dueland, et al.
Published: (1993-06-01)

Endotoxin, TNF, and IL-1 decrease cholesterol 7 alpha-hydroxylase mRNA levels and activity
by: K R Feingold, et al.
Published: (1996-02-01)

Synchronous circadian rhythms of mRNA levels and activities of cholesterol 7 alpha-hydroxylase in the rabbit and rat.
by: M Kai, et al.
Published: (1995-02-01)

Bile acid excretion and cholesterol 7 alpha-hydroxylase expression in hypercholesterolemia-resistant rabbits
by: JA Poorman, et al.
Published: (1993-10-01)

Regulation of cholesterol 7 alpha-hydroxylase expression by sterols in primary rat hepatocyte cultures.
by: K.C. Doerner, et al.
Published: (1995-06-01)

Bile acid synthesis. VI. Regulation of cholesterol 7 alpha-hydroxylase by taurocholate and mevalonate.
by: WM Pandak, et al.
Published: (1992-05-01)

Cholesterol 7 alpha-hydroxylase from human liver: partial purification and reconstruction into defined phospholipid-cholesterol vesicles.
by: S K Erickson, et al.
Published: (1981-07-01)

Up to date on cholesterol 7 alpha-hydroxylase (CYP7A1) in bile acid synthesis
by: John Y.L. Chiang, et al.
Published: (2020-06-01)

Hormonal regulation of cholesterol 7alpha-hydroxylase specific activity, mRNA levels, and transcriptional activity in vivo in the rat
by: W M Pandak, et al.
Published: (1997-12-01)

Determination of hepatic cholesterol 7α-hydroxylase activity in man
by: G. Nicolau, et al.
Published: (1974-03-01)

Cholesterol 7 alpha-hydroxylase (CYP7A1) gene polymorphisms are associated with increased LDL-cholesterol levels and the incidence of subclinical atherosclerosis
by: Gilberto Vargas-Alarcón, et al.
Published: (2024-08-01)

Studies on the link between HMG-CoA reductase and cholesterol 7 alpha-hydroxylase in rat liver.
by: I Björkhem, et al.
Published: (1988-02-01)

Structural characterization of human cholesterol 7α-hydroxylase
by: Wolfram Tempel, et al.
Published: (2014-09-01)

Regulation of rat liver microsomal cholesterol 7 alpha-hydroxylase: reversible inactivation by ATP + Mg2+ and a cytosolic activator.
by: C T Kwok, et al.
Published: (1981-05-01)

Regulation of bile acid synthesis. V. Inhibition of conversion of 7-dehydrocholesterol to cholesterol is associated with down-regulation of cholesterol 7 alpha-hydroxylase activity and inhibition of bile acid synthesis.
by: WM Pandak, et al.
Published: (1990-12-01)

Bile acid synthesis in man: assay of hepatic microsomal cholesterol 7 alpha-hydroxylase activity by isotope dilution-mass spectrometry.
by: K Einarsson, et al.
Published: (1986-11-01)

Regulation of bile acid synthesis in the rat: relationship between hepatic cholesterol 7 alpha-hydroxylase activity and portal bile acids.
by: K Fukushima, et al.
Published: (1995-02-01)

Differing effects of cholesterol and taurocholate on steady state hepatic HMG-CoA reductase and cholesterol 7 alpha-hydroxylase activities and mRNA levels in the rat.
by: S Shefer, et al.
Published: (1992-08-01)

Isotope derivative assay of microsomal cholesterol 7 α-hydroxylase
by: S. Shefer, et al.
Published: (1975-03-01)

Expression of cholesterol-7α-hydroxylase in murine macrophages prevents cholesterol loading by acetyl-LDL
by: Gina L. Moore, et al.
Published: (2002-04-01)

Stimulation of cholesterol 7α-hydroxylase by phenobarbital in two strains of rats
by: S. Shefer, et al.
Published: (1972-01-01)

Regulation of cholesterol-7α-hydroxylase: BAREly missing a SHP
by: Roger A. Davis, et al.
Published: (2002-04-01)

Enzyme activity assay for cholesterol 27-hydroxylase in mitochondria
by: Xiaobo Li, et al.
Published: (2006-07-01)

Bile acid synthesis in the Smith-Lemli-Opitz syndrome: effects of dehydrocholesterols on cholesterol 7α-hydroxylase and 27-hydroxylase activities in rat liver
by: Akira Honda, et al.
Published: (1999-08-01)

The use of [7α-3H]- and [7α, 7β-3H]cholesterol in the enzymic assay of cholesterol 7α-hydroxylase
by: D B Johnson, et al.
Published: (1976-07-01)

Stimulation of HMG-CoA reductase as a consequence of phenobarbital-induced primary stimulation of cholesterol 7 alpha-hydroxylase in rat liver.
by: E Sudjana-Sugiaman, et al.
Published: (1994-02-01)

Repression of cholesterol 7 alpha-hydroxylase transcription by bile acids is mediated through protein kinase C in primary cultures of rat hepatocytes.
by: R T Stravitz, et al.
Published: (1995-06-01)