The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis.
Proteins from their native conformation convert into highly ordered fibrillar aggregation under particular conditions; that are described as amyloid fibrils. α-Synuclein (α-Syn) is a small natively unfolded protein that its fibrillation is the causative factor of Parkinson's disease. One import...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0217801 |
| _version_ | 1818571851284086784 |
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| author | Shokouh Honarmand Bahareh Dabirmanesh Massoud Amanlou Khosro Khajeh |
| author_facet | Shokouh Honarmand Bahareh Dabirmanesh Massoud Amanlou Khosro Khajeh |
| author_sort | Shokouh Honarmand |
| collection | DOAJ |
| description | Proteins from their native conformation convert into highly ordered fibrillar aggregation under particular conditions; that are described as amyloid fibrils. α-Synuclein (α-Syn) is a small natively unfolded protein that its fibrillation is the causative factor of Parkinson's disease. One important approach in the development of therapeutic agents is the use of small molecules (such as flavonoids) that could specifically and efficiently inhibit the aggregation process. In this study the effect of few herbal extract (Berberis, Quercus robur, Zizyphus vulgaris, Salix aegyptica) containing flavonoids were investigated on fibril formation of α-syn by using conventional methods such as ThT fluorescence, circular dichroism (CD) spectroscopy and transmission electron microscopy (TEM). The interaction of extracts were also analysed by surface plasmon resonance (SPR). Among extracts, Salix aegyptica revealed the highest inhibitory effect on fibril formation. As expected, Salix aegyptica extract also exhibited the highest affinity toward α-syn. Cell viability using MTT assay revealed that fibrils alone were more toxic than those containing the extract. Overall, we demonstrated that the affinity of compounds used in this study corresponds to their ability to arrest fibrillation and reduce cellular toxicity of α-syn fibrils. |
| first_indexed | 2024-12-14T18:49:45Z |
| format | Article |
| id | doaj.art-234a993338234aa8910da0deb6d8be49 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T18:49:45Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-234a993338234aa8910da0deb6d8be492022-12-21T22:51:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01146e021780110.1371/journal.pone.0217801The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis.Shokouh HonarmandBahareh DabirmaneshMassoud AmanlouKhosro KhajehProteins from their native conformation convert into highly ordered fibrillar aggregation under particular conditions; that are described as amyloid fibrils. α-Synuclein (α-Syn) is a small natively unfolded protein that its fibrillation is the causative factor of Parkinson's disease. One important approach in the development of therapeutic agents is the use of small molecules (such as flavonoids) that could specifically and efficiently inhibit the aggregation process. In this study the effect of few herbal extract (Berberis, Quercus robur, Zizyphus vulgaris, Salix aegyptica) containing flavonoids were investigated on fibril formation of α-syn by using conventional methods such as ThT fluorescence, circular dichroism (CD) spectroscopy and transmission electron microscopy (TEM). The interaction of extracts were also analysed by surface plasmon resonance (SPR). Among extracts, Salix aegyptica revealed the highest inhibitory effect on fibril formation. As expected, Salix aegyptica extract also exhibited the highest affinity toward α-syn. Cell viability using MTT assay revealed that fibrils alone were more toxic than those containing the extract. Overall, we demonstrated that the affinity of compounds used in this study corresponds to their ability to arrest fibrillation and reduce cellular toxicity of α-syn fibrils.https://doi.org/10.1371/journal.pone.0217801 |
| spellingShingle | Shokouh Honarmand Bahareh Dabirmanesh Massoud Amanlou Khosro Khajeh The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis. PLoS ONE |
| title | The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis. |
| title_full | The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis. |
| title_fullStr | The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis. |
| title_full_unstemmed | The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis. |
| title_short | The interaction of several herbal extracts with α-synuclein: Fibril formation and surface plasmon resonance analysis. |
| title_sort | interaction of several herbal extracts with α synuclein fibril formation and surface plasmon resonance analysis |
| url | https://doi.org/10.1371/journal.pone.0217801 |
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