Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity
Infectious bursal disease (IBD), as a highly infectious immunosuppressive disease, causes severe economic losses in the poultry industry worldwide. <i>Saccharomyces cerevisiae</i> is an appealing vehicle used in oral vaccine formulations to safely and effectively deliver heterologous ant...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/11/12/1849 |
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| author | Huliang Li Deping Hua Qingxia Qu Hongwei Cao Zhehan Feng Na Liu Jinhai Huang Lei Zhang |
| author_facet | Huliang Li Deping Hua Qingxia Qu Hongwei Cao Zhehan Feng Na Liu Jinhai Huang Lei Zhang |
| author_sort | Huliang Li |
| collection | DOAJ |
| description | Infectious bursal disease (IBD), as a highly infectious immunosuppressive disease, causes severe economic losses in the poultry industry worldwide. <i>Saccharomyces cerevisiae</i> is an appealing vehicle used in oral vaccine formulations to safely and effectively deliver heterologous antigens. It can elicit systemic and mucosal responses. This study aims to explore the potential as oral an vaccine for <i>S. cerevisiae</i> expressing the capsid protein VP2 of IBDV. We constructed the recombinant <i>S. cerevisiae,</i> demonstrated that VP2 was displayed on the cell surface and had high immunoreactivity. By using the live ST1814G/Aga2-VP2 strain to immunize the mice, the results showed that recombinant <i>S. cerevisiae</i> significantly increased specific IgG and sIgA antibody titers, indicating the potential efficacy of vaccine-induced protection. These results suggested that the VP2 protein-expressing recombinant <i>S. cerevisiae</i> strain was a promising candidate oral subunit vaccine to prevent IBDV infection. |
| first_indexed | 2024-03-08T20:17:51Z |
| format | Article |
| id | doaj.art-234b702b5e674281a3af77c7d2568356 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-08T20:17:51Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-234b702b5e674281a3af77c7d25683562023-12-22T14:47:32ZengMDPI AGVaccines2076-393X2023-12-011112184910.3390/vaccines11121849Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective ImmunityHuliang Li0Deping Hua1Qingxia Qu2Hongwei Cao3Zhehan Feng4Na Liu5Jinhai Huang6Lei Zhang7School of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaSchool of Life Sciences, Tianjin University, Tianjin 300072, ChinaInfectious bursal disease (IBD), as a highly infectious immunosuppressive disease, causes severe economic losses in the poultry industry worldwide. <i>Saccharomyces cerevisiae</i> is an appealing vehicle used in oral vaccine formulations to safely and effectively deliver heterologous antigens. It can elicit systemic and mucosal responses. This study aims to explore the potential as oral an vaccine for <i>S. cerevisiae</i> expressing the capsid protein VP2 of IBDV. We constructed the recombinant <i>S. cerevisiae,</i> demonstrated that VP2 was displayed on the cell surface and had high immunoreactivity. By using the live ST1814G/Aga2-VP2 strain to immunize the mice, the results showed that recombinant <i>S. cerevisiae</i> significantly increased specific IgG and sIgA antibody titers, indicating the potential efficacy of vaccine-induced protection. These results suggested that the VP2 protein-expressing recombinant <i>S. cerevisiae</i> strain was a promising candidate oral subunit vaccine to prevent IBDV infection.https://www.mdpi.com/2076-393X/11/12/1849IBDVVP2oral immunizationvaccine |
| spellingShingle | Huliang Li Deping Hua Qingxia Qu Hongwei Cao Zhehan Feng Na Liu Jinhai Huang Lei Zhang Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity Vaccines IBDV VP2 oral immunization vaccine |
| title | Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity |
| title_full | Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity |
| title_fullStr | Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity |
| title_full_unstemmed | Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity |
| title_short | Oral Immunization with Recombinant <i>Saccharomyces cerevisiae</i> Expressing Viral Capsid Protein 2 of Infectious Bursal Disease Virus Induces Unique Specific Antibodies and Protective Immunity |
| title_sort | oral immunization with recombinant i saccharomyces cerevisiae i expressing viral capsid protein 2 of infectious bursal disease virus induces unique specific antibodies and protective immunity |
| topic | IBDV VP2 oral immunization vaccine |
| url | https://www.mdpi.com/2076-393X/11/12/1849 |
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