Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients

Background: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA m...

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Main Authors: Mohammad Mehdi HEIDARI, Mahboobe DERAKHSHANI, Fatemeh SEDIGHI, Seyed Khalil FORUZAN-NIA
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2017-10-01
Series:Iranian Journal of Public Health
Subjects:
Online Access:https://ijph.tums.ac.ir/index.php/ijph/article/view/11249
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author Mohammad Mehdi HEIDARI
Mahboobe DERAKHSHANI
Fatemeh SEDIGHI
Seyed Khalil FORUZAN-NIA
author_facet Mohammad Mehdi HEIDARI
Mahboobe DERAKHSHANI
Fatemeh SEDIGHI
Seyed Khalil FORUZAN-NIA
author_sort Mohammad Mehdi HEIDARI
collection DOAJ
description Background: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA mutations in atherosclerotic patients. Methods: In this case-control study, the variations of ten mitochondrial tRNA genes (about 50%) were investigated in 70 patients from October 2013 and June 2015 suffered from atherosclerosis. The related mitochondrial area was amplified using PCR methid. The mutation analysis was performed by Single Strand Conformational Polymorphism (SSCP) and Restriction Fragment Length Polymorphism (RFLP). All the positive samples were sequenced. Results: We found one novel heteroplasmic mutation (m.5725T>G) and three reported single nucleotide polymorphisms (SNPs) previously in other diseases including m.5568A>G, m.5711A>G and m.12308A>G. Conclusion: These tRNA mutations can alter their steady state level and affect the structure of tRNA. The role of mitochondrial tRNA mutations in the pathogenesis of atherosclerosis could potentially be important for the understanding of mitochondrial dysfunction in coronary atherosclerotic plaque formation.
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spelling doaj.art-234eb0ffe38a41f4aea5892c7d5086172022-12-21T23:50:41ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932017-10-014610Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis PatientsMohammad Mehdi HEIDARI0Mahboobe DERAKHSHANI1Fatemeh SEDIGHI2Seyed Khalil FORUZAN-NIA3Dept. of Biology, Faculty of Science, Yazd University, Yazd, IranDept. of Biology, Faculty of Science, Yazd University, Yazd, IranDept. of Biology, Faculty of Science, Yazd University, Yazd, IranDept. of Cardiac Surgery, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA mutations in atherosclerotic patients. Methods: In this case-control study, the variations of ten mitochondrial tRNA genes (about 50%) were investigated in 70 patients from October 2013 and June 2015 suffered from atherosclerosis. The related mitochondrial area was amplified using PCR methid. The mutation analysis was performed by Single Strand Conformational Polymorphism (SSCP) and Restriction Fragment Length Polymorphism (RFLP). All the positive samples were sequenced. Results: We found one novel heteroplasmic mutation (m.5725T>G) and three reported single nucleotide polymorphisms (SNPs) previously in other diseases including m.5568A>G, m.5711A>G and m.12308A>G. Conclusion: These tRNA mutations can alter their steady state level and affect the structure of tRNA. The role of mitochondrial tRNA mutations in the pathogenesis of atherosclerosis could potentially be important for the understanding of mitochondrial dysfunction in coronary atherosclerotic plaque formation.https://ijph.tums.ac.ir/index.php/ijph/article/view/11249AtherosclerosisMitochondrial tRNAMutationPCR-SSCP
spellingShingle Mohammad Mehdi HEIDARI
Mahboobe DERAKHSHANI
Fatemeh SEDIGHI
Seyed Khalil FORUZAN-NIA
Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
Iranian Journal of Public Health
Atherosclerosis
Mitochondrial tRNA
Mutation
PCR-SSCP
title Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
title_full Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
title_fullStr Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
title_full_unstemmed Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
title_short Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
title_sort mutation analysis of the mitochondrial trna genes in iranian coronary atherosclerosis patients
topic Atherosclerosis
Mitochondrial tRNA
Mutation
PCR-SSCP
url https://ijph.tums.ac.ir/index.php/ijph/article/view/11249
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