Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients
Background: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA m...
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Format: | Article |
Language: | English |
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Tehran University of Medical Sciences
2017-10-01
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Series: | Iranian Journal of Public Health |
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Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/11249 |
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author | Mohammad Mehdi HEIDARI Mahboobe DERAKHSHANI Fatemeh SEDIGHI Seyed Khalil FORUZAN-NIA |
author_facet | Mohammad Mehdi HEIDARI Mahboobe DERAKHSHANI Fatemeh SEDIGHI Seyed Khalil FORUZAN-NIA |
author_sort | Mohammad Mehdi HEIDARI |
collection | DOAJ |
description | Background: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA mutations in atherosclerotic patients.
Methods: In this case-control study, the variations of ten mitochondrial tRNA genes (about 50%) were investigated in 70 patients from October 2013 and June 2015 suffered from atherosclerosis. The related mitochondrial area was amplified using PCR methid. The mutation analysis was performed by Single Strand Conformational Polymorphism (SSCP) and Restriction Fragment Length Polymorphism (RFLP). All the positive samples were sequenced.
Results: We found one novel heteroplasmic mutation (m.5725T>G) and three reported single nucleotide polymorphisms (SNPs) previously in other diseases including m.5568A>G, m.5711A>G and m.12308A>G.
Conclusion: These tRNA mutations can alter their steady state level and affect the structure of tRNA. The role of mitochondrial tRNA mutations in the pathogenesis of atherosclerosis could potentially be important for the understanding of mitochondrial dysfunction in coronary atherosclerotic plaque formation. |
first_indexed | 2024-12-13T10:36:47Z |
format | Article |
id | doaj.art-234eb0ffe38a41f4aea5892c7d508617 |
institution | Directory Open Access Journal |
issn | 2251-6085 2251-6093 |
language | English |
last_indexed | 2024-12-13T10:36:47Z |
publishDate | 2017-10-01 |
publisher | Tehran University of Medical Sciences |
record_format | Article |
series | Iranian Journal of Public Health |
spelling | doaj.art-234eb0ffe38a41f4aea5892c7d5086172022-12-21T23:50:41ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932017-10-014610Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis PatientsMohammad Mehdi HEIDARI0Mahboobe DERAKHSHANI1Fatemeh SEDIGHI2Seyed Khalil FORUZAN-NIA3Dept. of Biology, Faculty of Science, Yazd University, Yazd, IranDept. of Biology, Faculty of Science, Yazd University, Yazd, IranDept. of Biology, Faculty of Science, Yazd University, Yazd, IranDept. of Cardiac Surgery, Afshar Hospital, Shahid Sadoughi University of Medical Sciences, Yazd, IranBackground: Atherosclerosis is a disease that affects large and medium size arteries in the body that underlies coronary heart disease. Several nucleotide changes in mitochondrial tRNA genes have been reported in various diseases. The purpose of the study was to identify hotspot mitochondrial tRNA mutations in atherosclerotic patients. Methods: In this case-control study, the variations of ten mitochondrial tRNA genes (about 50%) were investigated in 70 patients from October 2013 and June 2015 suffered from atherosclerosis. The related mitochondrial area was amplified using PCR methid. The mutation analysis was performed by Single Strand Conformational Polymorphism (SSCP) and Restriction Fragment Length Polymorphism (RFLP). All the positive samples were sequenced. Results: We found one novel heteroplasmic mutation (m.5725T>G) and three reported single nucleotide polymorphisms (SNPs) previously in other diseases including m.5568A>G, m.5711A>G and m.12308A>G. Conclusion: These tRNA mutations can alter their steady state level and affect the structure of tRNA. The role of mitochondrial tRNA mutations in the pathogenesis of atherosclerosis could potentially be important for the understanding of mitochondrial dysfunction in coronary atherosclerotic plaque formation.https://ijph.tums.ac.ir/index.php/ijph/article/view/11249AtherosclerosisMitochondrial tRNAMutationPCR-SSCP |
spellingShingle | Mohammad Mehdi HEIDARI Mahboobe DERAKHSHANI Fatemeh SEDIGHI Seyed Khalil FORUZAN-NIA Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients Iranian Journal of Public Health Atherosclerosis Mitochondrial tRNA Mutation PCR-SSCP |
title | Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients |
title_full | Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients |
title_fullStr | Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients |
title_full_unstemmed | Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients |
title_short | Mutation Analysis of the Mitochondrial tRNA Genes in Iranian Coronary Atherosclerosis Patients |
title_sort | mutation analysis of the mitochondrial trna genes in iranian coronary atherosclerosis patients |
topic | Atherosclerosis Mitochondrial tRNA Mutation PCR-SSCP |
url | https://ijph.tums.ac.ir/index.php/ijph/article/view/11249 |
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