Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells
Adenosine 3′, 5′-monophosphate (cAMP) is an intracellular mediator of the effects of external signals on target cells. Using immunohistochemistry, it has been observed that orthodontic forces alter the cAMP content in several cell types in the cat periodontal ligament (PDL). The objective of this st...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayinevi
1997-12-01
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| Series: | Turkish Journal of Orthodontics |
| Subjects: | |
| Online Access: |
http://www.turkjorthod.org/archives/archive-detail/article-preview/dstrbuton-of-cyclc-amp-n-mechancally-stressed-pero/54096
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| _version_ | 1827888582685097984 |
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| author | Hakan Gögen Zeev Davıdovıtch |
| author_facet | Hakan Gögen Zeev Davıdovıtch |
| author_sort | Hakan Gögen |
| collection | DOAJ |
| description | Adenosine 3′, 5′-monophosphate (cAMP) is an intracellular mediator of the effects of external signals on target cells. Using immunohistochemistry, it has been observed that orthodontic forces alter the cAMP content in several cell types in the cat periodontal ligament (PDL). The objective of this study was to determine the effects of orthodontic forces on the intracellular distribution of cAMP in PDL fibroblasts. Twelve, one year old cats were treated by 80 g tipping force to one maxillary canine for 1. 7 and 14 days. Maxillary horizontal sections (5 m) were stained for cAMP. Cellular staining intensity was measured as percent light transmitted by computer aided microphotometry using the MAPS program (Zeiss). Both the nuclei and the cytoplasm of the cells on the treated side stained darker with respect to the control side, except for the nuclei at 1 day. The cytoplasm stained darker than the nuclei on the treated side at all time intervals. These results indicate that mechanical force affects the cAMP content and its intracellular distribution in periodontal cells. |
| first_indexed | 2024-03-12T20:35:55Z |
| format | Article |
| id | doaj.art-23528f57f36b4c528dc8721f28a42cc5 |
| institution | Directory Open Access Journal |
| issn | 2528-9659 2148-9505 |
| language | English |
| last_indexed | 2024-03-12T20:35:55Z |
| publishDate | 1997-12-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Turkish Journal of Orthodontics |
| spelling | doaj.art-23528f57f36b4c528dc8721f28a42cc52023-08-01T13:05:52ZengGalenos YayineviTurkish Journal of Orthodontics2528-96592148-95051997-12-0110326527110.13076/1300-3550-10-3-26513049054Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast CellsHakan Gögen0Zeev Davıdovıtch1 Professor, Department of Orthodontics Faculty of Dentistry Ankara University Professor, Department of Orthodontics, School of Dental Medicine, Harvard University Adenosine 3′, 5′-monophosphate (cAMP) is an intracellular mediator of the effects of external signals on target cells. Using immunohistochemistry, it has been observed that orthodontic forces alter the cAMP content in several cell types in the cat periodontal ligament (PDL). The objective of this study was to determine the effects of orthodontic forces on the intracellular distribution of cAMP in PDL fibroblasts. Twelve, one year old cats were treated by 80 g tipping force to one maxillary canine for 1. 7 and 14 days. Maxillary horizontal sections (5 m) were stained for cAMP. Cellular staining intensity was measured as percent light transmitted by computer aided microphotometry using the MAPS program (Zeiss). Both the nuclei and the cytoplasm of the cells on the treated side stained darker with respect to the control side, except for the nuclei at 1 day. The cytoplasm stained darker than the nuclei on the treated side at all time intervals. These results indicate that mechanical force affects the cAMP content and its intracellular distribution in periodontal cells. http://www.turkjorthod.org/archives/archive-detail/article-preview/dstrbuton-of-cyclc-amp-n-mechancally-stressed-pero/54096 camporthodontic tooth movement.pdl cells |
| spellingShingle | Hakan Gögen Zeev Davıdovıtch Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells Turkish Journal of Orthodontics camp orthodontic tooth movement. pdl cells |
| title | Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells |
| title_full | Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells |
| title_fullStr | Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells |
| title_full_unstemmed | Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells |
| title_short | Dıstrıbutıon Of Cyclıc-Amp In Mechanıcally-Stressed Perıodontal Fıbroblast Cells |
| title_sort | distribution of cyclic amp in mechanically stressed periodontal fibroblast cells |
| topic | camp orthodontic tooth movement. pdl cells |
| url |
http://www.turkjorthod.org/archives/archive-detail/article-preview/dstrbuton-of-cyclc-amp-n-mechancally-stressed-pero/54096
|
| work_keys_str_mv | AT hakangogen dıstrıbutıonofcyclıcampinmechanıcallystressedperıodontalfıbroblastcells AT zeevdavıdovıtch dıstrıbutıonofcyclıcampinmechanıcallystressedperıodontalfıbroblastcells |