The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells
Annexin A1 (ANXA1) has been reported to promote tumor growth and resistance to chemotherapy drugs in lung cancer cells. In this study, we focused on the association of ANXA1 and chemosensitivity with a third generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), Osimertini...
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MDPI AG
2021-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/16/4106 |
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| author | Min-Chun Chuang Jr-Hau Lung Yi-Chuan Chen Yu-Ching Lin Ya-Chin Li Ming-Szu Hung |
| author_facet | Min-Chun Chuang Jr-Hau Lung Yi-Chuan Chen Yu-Ching Lin Ya-Chin Li Ming-Szu Hung |
| author_sort | Min-Chun Chuang |
| collection | DOAJ |
| description | Annexin A1 (ANXA1) has been reported to promote tumor growth and resistance to chemotherapy drugs in lung cancer cells. In this study, we focused on the association of ANXA1 and chemosensitivity with a third generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), Osimertinib, in lung cancer cells with EGFR mutations. The overexpression of ANXA1 was observed in the lung cancer cells studied. The downregulation of ANXA1 with small interference RNA (siRNA) decreased the growth of lung cancer cells. In lung cancer cells with EGFR mutations, the knockdown of ANXA1 increased the chemosensitivity to Osimertinib, and decreased the tumorigenesis, invasion and migration of lung cancer cells. Further study showed that the knockdown of ANXA1 inhibited the phosphorylation of EGFR and down-stream Akt pathways and promoted apoptosis in lung cancer cells treated with Osimertinib. A mice xenograft lung cancer model was established in our study and showed that ANXA1 siRNA enhanced the effects of Osimertinib in vivo. Our study results showed that ANXA1 plays critical roles in chemosensitivity to EGFR-TKI in lung cancer cells with the EGFR mutation. Our efforts may be used in the development of lung cancer treatment strategies in the future. |
| first_indexed | 2024-03-10T08:56:15Z |
| format | Article |
| id | doaj.art-235296dac0124fb9abaeeb618c4febfb |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T08:56:15Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-235296dac0124fb9abaeeb618c4febfb2023-11-22T07:03:52ZengMDPI AGCancers2072-66942021-08-011316410610.3390/cancers13164106The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer CellsMin-Chun Chuang0Jr-Hau Lung1Yi-Chuan Chen2Yu-Ching Lin3Ya-Chin Li4Ming-Szu Hung5Department of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital, Chiayi 61363, TaiwanDepartment of Medical Research, Chang Gung Memorial Hospital, Chiayi 61363, TaiwanDepartment of Emergency Medicine, Madou Sin-Lau Hospital, The Presbyterian Church in Taiwan, Tainan 72100, TaiwanDepartment of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital, Chiayi 61363, TaiwanDepartment of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital, Chiayi 61363, TaiwanDepartment of Pulmonary and Critical Care Medicine, Chang Gung Memorial Hospital, Chiayi 61363, TaiwanAnnexin A1 (ANXA1) has been reported to promote tumor growth and resistance to chemotherapy drugs in lung cancer cells. In this study, we focused on the association of ANXA1 and chemosensitivity with a third generation epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI), Osimertinib, in lung cancer cells with EGFR mutations. The overexpression of ANXA1 was observed in the lung cancer cells studied. The downregulation of ANXA1 with small interference RNA (siRNA) decreased the growth of lung cancer cells. In lung cancer cells with EGFR mutations, the knockdown of ANXA1 increased the chemosensitivity to Osimertinib, and decreased the tumorigenesis, invasion and migration of lung cancer cells. Further study showed that the knockdown of ANXA1 inhibited the phosphorylation of EGFR and down-stream Akt pathways and promoted apoptosis in lung cancer cells treated with Osimertinib. A mice xenograft lung cancer model was established in our study and showed that ANXA1 siRNA enhanced the effects of Osimertinib in vivo. Our study results showed that ANXA1 plays critical roles in chemosensitivity to EGFR-TKI in lung cancer cells with the EGFR mutation. Our efforts may be used in the development of lung cancer treatment strategies in the future.https://www.mdpi.com/2072-6694/13/16/4106lung cancerannexin A1target therapy |
| spellingShingle | Min-Chun Chuang Jr-Hau Lung Yi-Chuan Chen Yu-Ching Lin Ya-Chin Li Ming-Szu Hung The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells Cancers lung cancer annexin A1 target therapy |
| title | The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells |
| title_full | The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells |
| title_fullStr | The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells |
| title_full_unstemmed | The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells |
| title_short | The Association of Annexin A1 and Chemosensitivity to Osimertinib in Lung Cancer Cells |
| title_sort | association of annexin a1 and chemosensitivity to osimertinib in lung cancer cells |
| topic | lung cancer annexin A1 target therapy |
| url | https://www.mdpi.com/2072-6694/13/16/4106 |
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