Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix.
The extracellular matrix (ECM) plays a vital role in maintaining normal tissue function. Collagens are major components of the ECM and there is a tight equilibrium between degradation and formation of these proteins ensuring tissue health and homeostasis. As a consequence of tissue turnover, small c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5875766?pdf=render |
| _version_ | 1828200649118973952 |
|---|---|
| author | Stephanie N Kehlet Nicholas Willumsen Gabriele Armbrecht Roswitha Dietzel Susanne Brix Kim Henriksen Morten A Karsdal |
| author_facet | Stephanie N Kehlet Nicholas Willumsen Gabriele Armbrecht Roswitha Dietzel Susanne Brix Kim Henriksen Morten A Karsdal |
| author_sort | Stephanie N Kehlet |
| collection | DOAJ |
| description | The extracellular matrix (ECM) plays a vital role in maintaining normal tissue function. Collagens are major components of the ECM and there is a tight equilibrium between degradation and formation of these proteins ensuring tissue health and homeostasis. As a consequence of tissue turnover, small collagen fragments are released into the circulation, which act as important biomarkers in the study of certain tissue-related remodeling factors in health and disease. The aim of this study was to establish an age-related collagen turnover profile of the main collagens of the interstitial matrix (type I and III collagen) and basement membrane (type IV collagen) in healthy men and women. By using well-characterized competitive ELISA-assays, we assessed specific fragments of degraded (C1M, C3M, C4M) and formed (PINP, Pro-C3, P4NP7S) type I, III and IV collagen in serum from 617 healthy men and women ranging in ages from 22 to 86. Subjects were divided into 5-year age groups according to their sex and age. Groups were compared using Kruskal-Wallis adjusted for Dunn's multiple comparisons test and Mann-Whitney t-test. Age-specific changes in collagen turnover was most profound for type I collagen. PINP levels decreased in men with advancing age, whereas in women, the level decreased in early adulthood followed by an increase around the age of menopause (age 40-60). Sex-specific changes in type I, III and IV collagen turnover was present at the age around menopause (age 40-60) with women having an increased turnover. In summary, collagen turnover is affected by age and sex with the interstitial matrix and the basement membrane being differently regulated. The observed changes needs to be accounted for when measuring ECM related biomarkers in clinical studies. |
| first_indexed | 2024-04-12T11:17:37Z |
| format | Article |
| id | doaj.art-2352a6081e274f6288906af011bdea09 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:17:37Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2352a6081e274f6288906af011bdea092022-12-22T03:35:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019445810.1371/journal.pone.0194458Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix.Stephanie N KehletNicholas WillumsenGabriele ArmbrechtRoswitha DietzelSusanne BrixKim HenriksenMorten A KarsdalThe extracellular matrix (ECM) plays a vital role in maintaining normal tissue function. Collagens are major components of the ECM and there is a tight equilibrium between degradation and formation of these proteins ensuring tissue health and homeostasis. As a consequence of tissue turnover, small collagen fragments are released into the circulation, which act as important biomarkers in the study of certain tissue-related remodeling factors in health and disease. The aim of this study was to establish an age-related collagen turnover profile of the main collagens of the interstitial matrix (type I and III collagen) and basement membrane (type IV collagen) in healthy men and women. By using well-characterized competitive ELISA-assays, we assessed specific fragments of degraded (C1M, C3M, C4M) and formed (PINP, Pro-C3, P4NP7S) type I, III and IV collagen in serum from 617 healthy men and women ranging in ages from 22 to 86. Subjects were divided into 5-year age groups according to their sex and age. Groups were compared using Kruskal-Wallis adjusted for Dunn's multiple comparisons test and Mann-Whitney t-test. Age-specific changes in collagen turnover was most profound for type I collagen. PINP levels decreased in men with advancing age, whereas in women, the level decreased in early adulthood followed by an increase around the age of menopause (age 40-60). Sex-specific changes in type I, III and IV collagen turnover was present at the age around menopause (age 40-60) with women having an increased turnover. In summary, collagen turnover is affected by age and sex with the interstitial matrix and the basement membrane being differently regulated. The observed changes needs to be accounted for when measuring ECM related biomarkers in clinical studies.http://europepmc.org/articles/PMC5875766?pdf=render |
| spellingShingle | Stephanie N Kehlet Nicholas Willumsen Gabriele Armbrecht Roswitha Dietzel Susanne Brix Kim Henriksen Morten A Karsdal Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix. PLoS ONE |
| title | Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix. |
| title_full | Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix. |
| title_fullStr | Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix. |
| title_full_unstemmed | Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix. |
| title_short | Age-related collagen turnover of the interstitial matrix and basement membrane: Implications of age- and sex-dependent remodeling of the extracellular matrix. |
| title_sort | age related collagen turnover of the interstitial matrix and basement membrane implications of age and sex dependent remodeling of the extracellular matrix |
| url | http://europepmc.org/articles/PMC5875766?pdf=render |
| work_keys_str_mv | AT stephanienkehlet agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix AT nicholaswillumsen agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix AT gabrielearmbrecht agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix AT roswithadietzel agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix AT susannebrix agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix AT kimhenriksen agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix AT mortenakarsdal agerelatedcollagenturnoveroftheinterstitialmatrixandbasementmembraneimplicationsofageandsexdependentremodelingoftheextracellularmatrix |