Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice
Oxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is <i>Artocarpus lakoocha</i>, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not...
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MDPI AG
2023-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/6/1709 |
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| author | Hung Gia Tran Aussavashai Shuayprom Patipark Kueanjinda Asada Leelahavanichkul Prapai Wongsinkongman Siriwan Chaisomboonpan Apiwat Tawatsin Kriangsak Ruchusatsawat Jongkonnee Wongpiyabovorn |
| author_facet | Hung Gia Tran Aussavashai Shuayprom Patipark Kueanjinda Asada Leelahavanichkul Prapai Wongsinkongman Siriwan Chaisomboonpan Apiwat Tawatsin Kriangsak Ruchusatsawat Jongkonnee Wongpiyabovorn |
| author_sort | Hung Gia Tran |
| collection | DOAJ |
| description | Oxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is <i>Artocarpus lakoocha</i>, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not been clearly demonstrated. Therefore, we investigated the anti-inflammatory effects of ORV on dermatitis model. The effect of ORV was examined on human immortalized and primary skin cells exposed to bacterial components including peptidoglycan (PGN) and lipopolysaccharide (LPS) and 2,4-Dinitrochlorobenzene (DNCB)-induced dermatitis mouse model. PGN and LPS were used to induce inflammation on immortalized keratinocytes (HaCaT) and human epidermal keratinocytes (HEKa). We then performed MTT assay, Annexin V and PI assay, cell cycle analysis, real-time PCR, ELISA and Western blot in these in vitro models. H&E staining, immunohistochemistry (IHC) staining with CD3, CD4 and CD8 markers were used to evaluate the effects of ORV in in vivo model of skin inflammation using BALB/c mice. Pretreatment of HaCaT and HEKa cells with ORV inhibited pro-inflammatory cytokine production through inhibition of NF-κB pathway. In DNCB-induced dermatitis mouse model, ORV treatment reduced lesion severity, and skin thickness and numbers of CD3, CD4 and CD8 T cells in the sensitized skin of mice. In conclusion, it has been demonstrated that ORV treatment can ameliorate inflammation in the in vitro models of skin inflammation and in vivo models of dermatitis, suggesting a therapeutic potential of ORV for treatment of skin diseases particularly eczema. |
| first_indexed | 2024-03-11T02:02:53Z |
| format | Article |
| id | doaj.art-23534d5a0c474522b63d8f80b0e2db9a |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T02:02:53Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
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| series | Pharmaceutics |
| spelling | doaj.art-23534d5a0c474522b63d8f80b0e2db9a2023-11-18T12:05:22ZengMDPI AGPharmaceutics1999-49232023-06-01156170910.3390/pharmaceutics15061709Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis MiceHung Gia Tran0Aussavashai Shuayprom1Patipark Kueanjinda2Asada Leelahavanichkul3Prapai Wongsinkongman4Siriwan Chaisomboonpan5Apiwat Tawatsin6Kriangsak Ruchusatsawat7Jongkonnee Wongpiyabovorn8Graduate Program in Clinical Sciences, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandCenter of Excellence in Immune-Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandCenter of Excellence on Translational Research in Inflammation and Immunology (CETRII), Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandDepartment of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandDepartment of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandDepartment of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, ThailandCenter of Excellence in Immune-Mediated Diseases, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, ThailandOxyresveratrol (ORV) is one of the novel antioxidants having been extensively studied in recent years. One of the main sources of ORV is <i>Artocarpus lakoocha</i>, which has been used in traditional medicine in Thailand for decades. However, the role of ORV in skin inflammation has not been clearly demonstrated. Therefore, we investigated the anti-inflammatory effects of ORV on dermatitis model. The effect of ORV was examined on human immortalized and primary skin cells exposed to bacterial components including peptidoglycan (PGN) and lipopolysaccharide (LPS) and 2,4-Dinitrochlorobenzene (DNCB)-induced dermatitis mouse model. PGN and LPS were used to induce inflammation on immortalized keratinocytes (HaCaT) and human epidermal keratinocytes (HEKa). We then performed MTT assay, Annexin V and PI assay, cell cycle analysis, real-time PCR, ELISA and Western blot in these in vitro models. H&E staining, immunohistochemistry (IHC) staining with CD3, CD4 and CD8 markers were used to evaluate the effects of ORV in in vivo model of skin inflammation using BALB/c mice. Pretreatment of HaCaT and HEKa cells with ORV inhibited pro-inflammatory cytokine production through inhibition of NF-κB pathway. In DNCB-induced dermatitis mouse model, ORV treatment reduced lesion severity, and skin thickness and numbers of CD3, CD4 and CD8 T cells in the sensitized skin of mice. In conclusion, it has been demonstrated that ORV treatment can ameliorate inflammation in the in vitro models of skin inflammation and in vivo models of dermatitis, suggesting a therapeutic potential of ORV for treatment of skin diseases particularly eczema.https://www.mdpi.com/1999-4923/15/6/1709oxyresveratrolskin inflammationeczemadermatitisanti-inflammationanti-proliferation |
| spellingShingle | Hung Gia Tran Aussavashai Shuayprom Patipark Kueanjinda Asada Leelahavanichkul Prapai Wongsinkongman Siriwan Chaisomboonpan Apiwat Tawatsin Kriangsak Ruchusatsawat Jongkonnee Wongpiyabovorn Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice Pharmaceutics oxyresveratrol skin inflammation eczema dermatitis anti-inflammation anti-proliferation |
| title | Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice |
| title_full | Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice |
| title_fullStr | Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice |
| title_full_unstemmed | Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice |
| title_short | Oxyresveratrol Attenuates Inflammation in Human Keratinocyte via Regulating NF-kB Signaling and Ameliorates Eczematous Lesion in DNCB-Induced Dermatitis Mice |
| title_sort | oxyresveratrol attenuates inflammation in human keratinocyte via regulating nf kb signaling and ameliorates eczematous lesion in dncb induced dermatitis mice |
| topic | oxyresveratrol skin inflammation eczema dermatitis anti-inflammation anti-proliferation |
| url | https://www.mdpi.com/1999-4923/15/6/1709 |
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