Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species
This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719776/?tool=EBI |
| _version_ | 1798035366631964672 |
|---|---|
| author | Jacqueline de Oliveira Zoccolotti Alberto José Cavalheiro Camilla Olga Tasso Beatriz Ribeiro Ribas Túlio Morandin Ferrisse Janaina Habib Jorge |
| author_facet | Jacqueline de Oliveira Zoccolotti Alberto José Cavalheiro Camilla Olga Tasso Beatriz Ribeiro Ribas Túlio Morandin Ferrisse Janaina Habib Jorge |
| author_sort | Jacqueline de Oliveira Zoccolotti |
| collection | DOAJ |
| description | This study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells. |
| first_indexed | 2024-04-11T20:56:14Z |
| format | Article |
| id | doaj.art-2356ccf7caca4edaba73d5e4d7021afc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T20:56:14Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2356ccf7caca4edaba73d5e4d7021afc2022-12-22T04:03:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011612Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya speciesJacqueline de Oliveira ZoccolottiAlberto José CavalheiroCamilla Olga TassoBeatriz Ribeiro RibasTúlio Morandin FerrisseJanaina Habib JorgeThis study evaluated the efficacy of Cryptocarya spp extracts on biofilm of Candida albicans and its biocompatibility. Mature biofilm of C. albicans was formed on denture base acrylic resin samples and the fungicidal effect of the extracts was evaluated by Alamar Blue® assay, counting colony-forming units (CFU/mL) and confocal laser scanning microscopy (CLSM). Cytotoxicity of extracts from Cryptocarya species was evaluated by AlamarBlue® assay, using normal oral keratinocytes (NOK) cells. In additional, Analysis of plant extracts by ultra-high-performance liquid chromatography–diode array detector–tandem mass spectrometry (UPLC-DAD-MS) was performed. The results showed significant reduction in the cellular metabolism and in the number of CFU/mL of C. albicans (p<0.05). The concentration of 0.045 g/mL completely inhibited the number of CFU/mL. Regarding cytotoxicity, all extracts decreased cell viability compared to the control group. CLSM analysis showed predominance of live cells, but with a great difference between the groups. Antimicrobial activity of extracts from Cryptocarya on C. albicans biofilm was confirmed. However, all extracts showed toxicity on NOK cells.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719776/?tool=EBI |
| spellingShingle | Jacqueline de Oliveira Zoccolotti Alberto José Cavalheiro Camilla Olga Tasso Beatriz Ribeiro Ribas Túlio Morandin Ferrisse Janaina Habib Jorge Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species PLoS ONE |
| title | Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species |
| title_full | Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species |
| title_fullStr | Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species |
| title_full_unstemmed | Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species |
| title_short | Antimicrobial efficacy and biocompatibility of extracts from Cryptocarya species |
| title_sort | antimicrobial efficacy and biocompatibility of extracts from cryptocarya species |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8719776/?tool=EBI |
| work_keys_str_mv | AT jacquelinedeoliveirazoccolotti antimicrobialefficacyandbiocompatibilityofextractsfromcryptocaryaspecies AT albertojosecavalheiro antimicrobialefficacyandbiocompatibilityofextractsfromcryptocaryaspecies AT camillaolgatasso antimicrobialefficacyandbiocompatibilityofextractsfromcryptocaryaspecies AT beatrizribeiroribas antimicrobialefficacyandbiocompatibilityofextractsfromcryptocaryaspecies AT tuliomorandinferrisse antimicrobialefficacyandbiocompatibilityofextractsfromcryptocaryaspecies AT janainahabibjorge antimicrobialefficacyandbiocompatibilityofextractsfromcryptocaryaspecies |