Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring
Abstract Streptomyces species are intensively studied for their ability to produce a variety of natural products. However, conditions influencing and leading to product formation are often not completely recognized. Therefore, in this study, high‐throughput online monitoring is presented as a powerf...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2023-01-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202100151 |
| _version_ | 1797961259448008704 |
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| author | Maurice Finger Fabio Sentek Lukas Hartmann Ana M. Palacio‐Barrera Ivan Schlembach Miriam A. Rosenbaum Jochen Büchs |
| author_facet | Maurice Finger Fabio Sentek Lukas Hartmann Ana M. Palacio‐Barrera Ivan Schlembach Miriam A. Rosenbaum Jochen Büchs |
| author_sort | Maurice Finger |
| collection | DOAJ |
| description | Abstract Streptomyces species are intensively studied for their ability to produce a variety of natural products. However, conditions influencing and leading to product formation are often not completely recognized. Therefore, in this study, high‐throughput online monitoring is presented as a powerful tool to gain in‐depth understanding of the cultivation of the model organism Streptomyces coelicolor A3(2). Through online measurements of oxygen transfer rate and autofluorescence, valuable information about availability of nutrients and product formation patterns of the pigments actinorhodin and undecylprodigiosin can be obtained and explained. Therefore, it is possible to determine the onset of pigmentation and to study in detail the influencing factors thereof. One factor identified in this study is the filling volume of the cultivation vessel. Slight variations led to varying pigmentation levels. By combining optical and metabolic online monitoring techniques, the correlation of the filling volume with pigmentation could be explained as a result of different growth trajectories caused by varying specific power inputs and their influence on the pellet formation of the filamentous system. Finally, experiments with the addition of supernatant from unpigmented and pigmented cultures could highlight the applicability of the presented approach to study quorum sensing and cell‐cell interaction. |
| first_indexed | 2024-04-11T00:56:13Z |
| format | Article |
| id | doaj.art-235954a2de3247558ef9771e5703eea9 |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-11T00:56:13Z |
| publishDate | 2023-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-235954a2de3247558ef9771e5703eea92023-01-05T02:40:22ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632023-01-01231n/an/a10.1002/elsc.202100151Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoringMaurice Finger0Fabio Sentek1Lukas Hartmann2Ana M. Palacio‐Barrera3Ivan Schlembach4Miriam A. Rosenbaum5Jochen Büchs6AVT ‐ Biochemical Engineering RWTH Aachen University Aachen GermanyAVT ‐ Biochemical Engineering RWTH Aachen University Aachen GermanyAVT ‐ Biochemical Engineering RWTH Aachen University Aachen GermanyLeibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyLeibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyLeibniz Institute for Natural Product Research and Infection Biology Hans‐Knöll‐Institute Jena GermanyAVT ‐ Biochemical Engineering RWTH Aachen University Aachen GermanyAbstract Streptomyces species are intensively studied for their ability to produce a variety of natural products. However, conditions influencing and leading to product formation are often not completely recognized. Therefore, in this study, high‐throughput online monitoring is presented as a powerful tool to gain in‐depth understanding of the cultivation of the model organism Streptomyces coelicolor A3(2). Through online measurements of oxygen transfer rate and autofluorescence, valuable information about availability of nutrients and product formation patterns of the pigments actinorhodin and undecylprodigiosin can be obtained and explained. Therefore, it is possible to determine the onset of pigmentation and to study in detail the influencing factors thereof. One factor identified in this study is the filling volume of the cultivation vessel. Slight variations led to varying pigmentation levels. By combining optical and metabolic online monitoring techniques, the correlation of the filling volume with pigmentation could be explained as a result of different growth trajectories caused by varying specific power inputs and their influence on the pellet formation of the filamentous system. Finally, experiments with the addition of supernatant from unpigmented and pigmented cultures could highlight the applicability of the presented approach to study quorum sensing and cell‐cell interaction.https://doi.org/10.1002/elsc.202100151high‐throughputmicrotiter plateonline monitoringpigmentationStreptomyces coelicolor |
| spellingShingle | Maurice Finger Fabio Sentek Lukas Hartmann Ana M. Palacio‐Barrera Ivan Schlembach Miriam A. Rosenbaum Jochen Büchs Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring Engineering in Life Sciences high‐throughput microtiter plate online monitoring pigmentation Streptomyces coelicolor |
| title | Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring |
| title_full | Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring |
| title_fullStr | Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring |
| title_full_unstemmed | Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring |
| title_short | Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high‐throughput online monitoring |
| title_sort | insights into streptomyces coelicolor a3 2 growth and pigment formation with high throughput online monitoring |
| topic | high‐throughput microtiter plate online monitoring pigmentation Streptomyces coelicolor |
| url | https://doi.org/10.1002/elsc.202100151 |
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