Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat
Fusarium head blight (FHB), caused by the fungus Fusarium graminearum Schwabe is an important disease of wheat that causes severe yield losses along with serious quality concerns. Incorporating the host resistance from either wild relatives, landraces, or exotic materials remains challenging and has...
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.946700/full |
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| author | Jinfeng Zhang Harsimardeep S. Gill Jyotirmoy Halder Navreet K. Brar Shaukat Ali Amy Bernardo Paul St. Amand Guihua Bai Brent Turnipseed Sunish K. Sehgal |
| author_facet | Jinfeng Zhang Harsimardeep S. Gill Jyotirmoy Halder Navreet K. Brar Shaukat Ali Amy Bernardo Paul St. Amand Guihua Bai Brent Turnipseed Sunish K. Sehgal |
| author_sort | Jinfeng Zhang |
| collection | DOAJ |
| description | Fusarium head blight (FHB), caused by the fungus Fusarium graminearum Schwabe is an important disease of wheat that causes severe yield losses along with serious quality concerns. Incorporating the host resistance from either wild relatives, landraces, or exotic materials remains challenging and has shown limited success. Therefore, a better understanding of the genetic basis of native FHB resistance in hard winter wheat (HWW) and combining it with major quantitative trait loci (QTLs) can facilitate the development of FHB-resistant cultivars. In this study, we evaluated a set of 257 breeding lines from the South Dakota State University (SDSU) breeding program to uncover the genetic basis of native FHB resistance in the US hard winter wheat. We conducted a multi-locus genome-wide association study (ML-GWAS) with 9,321 high-quality single-nucleotide polymorphisms (SNPs). A total of six distinct marker-trait associations (MTAs) were identified for the FHB disease index (DIS) on five different chromosomes including 2A, 2B, 3B, 4B, and 7A. Further, eight MTAs were identified for Fusarium-damaged kernels (FDK) on six chromosomes including 3B, 5A, 6B, 6D, 7A, and 7B. Out of the 14 significant MTAs, 10 were found in the proximity of previously reported regions for FHB resistance in different wheat classes and were validated in HWW, while four MTAs represent likely novel loci for FHB resistance. Accumulation of favorable alleles of reported MTAs resulted in significantly lower mean DIS and FDK score, demonstrating the additive effect of FHB resistance alleles. Candidate gene analysis for two important MTAs identified several genes with putative proteins of interest; however, further investigation of these regions is needed to identify genes conferring FHB resistance. The current study sheds light on the genetic basis of native FHB resistance in the US HWW germplasm and the resistant lines and MTAs identified in this study will be useful resources for FHB resistance breeding via marker-assisted selection. |
| first_indexed | 2024-04-13T20:18:53Z |
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| id | doaj.art-235f8d1eb83b48949f0cba2737b317d4 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T20:18:53Z |
| publishDate | 2022-07-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-235f8d1eb83b48949f0cba2737b317d42022-12-22T02:31:37ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.946700946700Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter WheatJinfeng Zhang0Harsimardeep S. Gill1Jyotirmoy Halder2Navreet K. Brar3Shaukat Ali4Amy Bernardo5Paul St. Amand6Guihua Bai7Brent Turnipseed8Sunish K. Sehgal9Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesUSDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, United StatesUSDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, United StatesUSDA-ARS, Hard Winter Wheat Genetics Research Unit, Manhattan, KS, United StatesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesDepartment of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United StatesFusarium head blight (FHB), caused by the fungus Fusarium graminearum Schwabe is an important disease of wheat that causes severe yield losses along with serious quality concerns. Incorporating the host resistance from either wild relatives, landraces, or exotic materials remains challenging and has shown limited success. Therefore, a better understanding of the genetic basis of native FHB resistance in hard winter wheat (HWW) and combining it with major quantitative trait loci (QTLs) can facilitate the development of FHB-resistant cultivars. In this study, we evaluated a set of 257 breeding lines from the South Dakota State University (SDSU) breeding program to uncover the genetic basis of native FHB resistance in the US hard winter wheat. We conducted a multi-locus genome-wide association study (ML-GWAS) with 9,321 high-quality single-nucleotide polymorphisms (SNPs). A total of six distinct marker-trait associations (MTAs) were identified for the FHB disease index (DIS) on five different chromosomes including 2A, 2B, 3B, 4B, and 7A. Further, eight MTAs were identified for Fusarium-damaged kernels (FDK) on six chromosomes including 3B, 5A, 6B, 6D, 7A, and 7B. Out of the 14 significant MTAs, 10 were found in the proximity of previously reported regions for FHB resistance in different wheat classes and were validated in HWW, while four MTAs represent likely novel loci for FHB resistance. Accumulation of favorable alleles of reported MTAs resulted in significantly lower mean DIS and FDK score, demonstrating the additive effect of FHB resistance alleles. Candidate gene analysis for two important MTAs identified several genes with putative proteins of interest; however, further investigation of these regions is needed to identify genes conferring FHB resistance. The current study sheds light on the genetic basis of native FHB resistance in the US HWW germplasm and the resistant lines and MTAs identified in this study will be useful resources for FHB resistance breeding via marker-assisted selection.https://www.frontiersin.org/articles/10.3389/fpls.2022.946700/fullFHB resistanceGBSmulti-locus GWAShard winter wheatwinter wheat breeding |
| spellingShingle | Jinfeng Zhang Harsimardeep S. Gill Jyotirmoy Halder Navreet K. Brar Shaukat Ali Amy Bernardo Paul St. Amand Guihua Bai Brent Turnipseed Sunish K. Sehgal Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat Frontiers in Plant Science FHB resistance GBS multi-locus GWAS hard winter wheat winter wheat breeding |
| title | Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat |
| title_full | Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat |
| title_fullStr | Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat |
| title_full_unstemmed | Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat |
| title_short | Multi-Locus Genome-Wide Association Studies to Characterize Fusarium Head Blight (FHB) Resistance in Hard Winter Wheat |
| title_sort | multi locus genome wide association studies to characterize fusarium head blight fhb resistance in hard winter wheat |
| topic | FHB resistance GBS multi-locus GWAS hard winter wheat winter wheat breeding |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.946700/full |
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