Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection
Abstract Sutures are applied almost universally at the site of trauma or surgery, making them an ideal platform to modulate the local, postoperative biological response, and improve surgical outcomes. To date, the only globally marketed drug‐eluting sutures are coated with triclosan for antibacteria...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-05-01
|
| Series: | Bioengineering & Translational Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/btm2.10204 |
| _version_ | 1818455368318058496 |
|---|---|
| author | Kunal S. Parikh Revaz Omiadze Aditya Josyula Richard Shi Nicole M. Anders Ping He Youseph Yazdi Peter J. McDonnell Laura M. Ensign Justin Hanes |
| author_facet | Kunal S. Parikh Revaz Omiadze Aditya Josyula Richard Shi Nicole M. Anders Ping He Youseph Yazdi Peter J. McDonnell Laura M. Ensign Justin Hanes |
| author_sort | Kunal S. Parikh |
| collection | DOAJ |
| description | Abstract Sutures are applied almost universally at the site of trauma or surgery, making them an ideal platform to modulate the local, postoperative biological response, and improve surgical outcomes. To date, the only globally marketed drug‐eluting sutures are coated with triclosan for antibacterial application in general surgery. Loading drug directly into the suture rather than coating the surface offers the potential to provide drug delivery functionality to microsurgical sutures and achieve sustained drug delivery without increasing suture thickness. However, conventional methods for drug incorporation directly into the suture adversely affect breaking strength. Thus, there are no market offerings for drug‐eluting sutures, drug‐coated, or otherwise, in ophthalmology, where very thin sutures are required. Sutures themselves help facilitate bacterial infection, and antibiotic eye drops are commonly prescribed to prevent infection after ocular surgeries. An antibiotic‐eluting suture may prevent bacterial colonization of sutures and preclude patient compliance issues with eye drops. We report twisting of hundreds of individual drug‐loaded, electrospun nanofibers into a single, ultra‐thin, multifilament suture capable of meeting both size and strength requirements for microsurgical ocular procedures. Nanofiber‐based polycaprolactone sutures demonstrated no loss in strength with loading of 8% levofloxacin, unlike monofilament sutures which lost more than 50% strength. Moreover, nanofiber‐based sutures retained strength with loading of a broad range of drugs, provided antibiotic delivery for 30 days in rat eyes, and prevented ocular infection in a rat model of bacterial keratitis. |
| first_indexed | 2024-12-14T22:09:40Z |
| format | Article |
| id | doaj.art-235fc883c4f34ff9aef8f69d95776b36 |
| institution | Directory Open Access Journal |
| issn | 2380-6761 |
| language | English |
| last_indexed | 2024-12-14T22:09:40Z |
| publishDate | 2021-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioengineering & Translational Medicine |
| spelling | doaj.art-235fc883c4f34ff9aef8f69d95776b362022-12-21T22:45:46ZengWileyBioengineering & Translational Medicine2380-67612021-05-0162n/an/a10.1002/btm2.10204Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infectionKunal S. Parikh0Revaz Omiadze1Aditya Josyula2Richard Shi3Nicole M. Anders4Ping He5Youseph Yazdi6Peter J. McDonnell7Laura M. Ensign8Justin Hanes9Center for Nanomedicine The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USADepartment of Oncology Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine Baltimore Maryland USADepartment of Oncology Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Bioengineering Innovation & Design Johns Hopkins University Baltimore Maryland USADepartment of Ophthalmology The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USACenter for Nanomedicine The Wilmer Eye Institute, Johns Hopkins University School of Medicine Baltimore Maryland USAAbstract Sutures are applied almost universally at the site of trauma or surgery, making them an ideal platform to modulate the local, postoperative biological response, and improve surgical outcomes. To date, the only globally marketed drug‐eluting sutures are coated with triclosan for antibacterial application in general surgery. Loading drug directly into the suture rather than coating the surface offers the potential to provide drug delivery functionality to microsurgical sutures and achieve sustained drug delivery without increasing suture thickness. However, conventional methods for drug incorporation directly into the suture adversely affect breaking strength. Thus, there are no market offerings for drug‐eluting sutures, drug‐coated, or otherwise, in ophthalmology, where very thin sutures are required. Sutures themselves help facilitate bacterial infection, and antibiotic eye drops are commonly prescribed to prevent infection after ocular surgeries. An antibiotic‐eluting suture may prevent bacterial colonization of sutures and preclude patient compliance issues with eye drops. We report twisting of hundreds of individual drug‐loaded, electrospun nanofibers into a single, ultra‐thin, multifilament suture capable of meeting both size and strength requirements for microsurgical ocular procedures. Nanofiber‐based polycaprolactone sutures demonstrated no loss in strength with loading of 8% levofloxacin, unlike monofilament sutures which lost more than 50% strength. Moreover, nanofiber‐based sutures retained strength with loading of a broad range of drugs, provided antibiotic delivery for 30 days in rat eyes, and prevented ocular infection in a rat model of bacterial keratitis.https://doi.org/10.1002/btm2.10204drug deliveryeye droplevofloxacinmedical devicenanofiberophthalmology |
| spellingShingle | Kunal S. Parikh Revaz Omiadze Aditya Josyula Richard Shi Nicole M. Anders Ping He Youseph Yazdi Peter J. McDonnell Laura M. Ensign Justin Hanes Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection Bioengineering & Translational Medicine drug delivery eye drop levofloxacin medical device nanofiber ophthalmology |
| title | Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection |
| title_full | Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection |
| title_fullStr | Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection |
| title_full_unstemmed | Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection |
| title_short | Ultra‐thin, high strength, antibiotic‐eluting sutures for prevention of ophthalmic infection |
| title_sort | ultra thin high strength antibiotic eluting sutures for prevention of ophthalmic infection |
| topic | drug delivery eye drop levofloxacin medical device nanofiber ophthalmology |
| url | https://doi.org/10.1002/btm2.10204 |
| work_keys_str_mv | AT kunalsparikh ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT revazomiadze ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT adityajosyula ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT richardshi ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT nicolemanders ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT pinghe ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT yousephyazdi ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT peterjmcdonnell ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT lauramensign ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection AT justinhanes ultrathinhighstrengthantibioticelutingsuturesforpreventionofophthalmicinfection |