Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence
The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically charac...
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| Format: | Article |
| Language: | English |
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Embrapa Informação Tecnológica
2013-03-01
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| Series: | Pesquisa Agropecuária Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005&lng=en&tlng=en |
| _version_ | 1818929481850552320 |
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| author | Carlos Alexandre Gomes Ribeiro Janaína Paula Marques Tanure Talles Eduardo Ferreira Maciel Everaldo Gonçalves de Barros |
| author_facet | Carlos Alexandre Gomes Ribeiro Janaína Paula Marques Tanure Talles Eduardo Ferreira Maciel Everaldo Gonçalves de Barros |
| author_sort | Carlos Alexandre Gomes Ribeiro |
| collection | DOAJ |
| description | The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the determination of genetic purity. |
| first_indexed | 2024-12-20T03:45:30Z |
| format | Article |
| id | doaj.art-23631622b598489e81ce7124f1a9353a |
| institution | Directory Open Access Journal |
| issn | 1678-3921 |
| language | English |
| last_indexed | 2024-12-20T03:45:30Z |
| publishDate | 2013-03-01 |
| publisher | Embrapa Informação Tecnológica |
| record_format | Article |
| series | Pesquisa Agropecuária Brasileira |
| spelling | doaj.art-23631622b598489e81ce7124f1a9353a2022-12-21T19:54:38ZengEmbrapa Informação TecnológicaPesquisa Agropecuária Brasileira1678-39212013-03-0148327027910.1590/S0100-204X2013000300005S0100-204X2013000300005Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequenceCarlos Alexandre Gomes Ribeiro0Janaína Paula Marques Tanure1Talles Eduardo Ferreira Maciel2Everaldo Gonçalves de Barros3Universidade Federal de ViçosaEmbrapaUniversidade Federal de ViçosaUniversidade Federal de ViçosaThe objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic data used for varietal registration. The 13 markers allowed the distinction of all analyzed cultivars. The low cost of the UTSP method, associated with its high accuracy, makes it ideal for the characterization of soybean cultivars and for the determination of genetic purity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005&lng=en&tlng=enGlycine maxproteção de cultivardiversidade genéticamétodo de genotipagemprobabilidade de identidade ao acaso |
| spellingShingle | Carlos Alexandre Gomes Ribeiro Janaína Paula Marques Tanure Talles Eduardo Ferreira Maciel Everaldo Gonçalves de Barros Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence Pesquisa Agropecuária Brasileira Glycine max proteção de cultivar diversidade genética método de genotipagem probabilidade de identidade ao acaso |
| title | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence |
| title_full | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence |
| title_fullStr | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence |
| title_full_unstemmed | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence |
| title_short | Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence |
| title_sort | molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence |
| topic | Glycine max proteção de cultivar diversidade genética método de genotipagem probabilidade de identidade ao acaso |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005&lng=en&tlng=en |
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