Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice
Objective: To evaluate the effects of Pasteurella multocida (P. multocida) vaccines on the expression and release of antibodies, interleukin (IL)-6 and IL-12 by serum. Methods: Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks. The vaccines were adjuvant...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Asian Pacific Journal of Tropical Medicine |
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| Online Access: | http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=5;spage=336;epage=341;aulast=Homayoon |
| _version_ | 1818136095554011136 |
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| author | Maryam Homayoon Yahya Tahamtan Mohammad Kargar Seyed Mohammad Hossein Hosseini Abbas Akhavan Sepahy |
| author_facet | Maryam Homayoon Yahya Tahamtan Mohammad Kargar Seyed Mohammad Hossein Hosseini Abbas Akhavan Sepahy |
| author_sort | Maryam Homayoon |
| collection | DOAJ |
| description | Objective: To evaluate the effects of Pasteurella multocida (P. multocida) vaccines on the expression and release of antibodies, interleukin (IL)-6 and IL-12 by serum. Methods: Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks. The vaccines were adjuvant with P. multocida A strain, P. multocida B strain and Salmonella typhimurium bacterial DNA (AbDNA, BbDNA and SbDNA for short, respectively). The animals were challenged 4 weeks after immunization. Blood of mice was collected to detect the change of specific antibody, IL-6, and IL-12 using ELISA. Results: The specific antibody and interleukins in the immunized group increased significantly compared to the control mice after vaccination and challenge (P<0.05). The highest release of these cytokines was obtained by P. multocida inactivated with iron and adjuvant with AbDNA at a concentration of 25 μg/mL. The antibody titer peak was 0.447 in mice vaccinated with iron-killed whole-cell antigen adjunct with AbDNA. The time-courses of release showed that bacterial DNA was able to stimulate IL-6 and IL-12 production more than alum (P<0.05). Conclusions: Our findings introduce that bacterial DNA is capable of releasing an immunological response with several cytokines. These indicate that bacterial DNA entrapped with killed P. multocida antigen is a new and effective adjuvant to enhance specific immunity and resistance of animal against the infectious pathogen, which could simplify the development of highly promising strong adjuvant. |
| first_indexed | 2024-12-11T09:34:58Z |
| format | Article |
| id | doaj.art-2363ad3bf9644b70b586540bc27ac765 |
| institution | Directory Open Access Journal |
| issn | 2352-4146 |
| language | English |
| last_indexed | 2024-12-11T09:34:58Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Medicine |
| spelling | doaj.art-2363ad3bf9644b70b586540bc27ac7652022-12-22T01:12:52ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Medicine2352-41462018-01-0111533634110.4103/1995-7645.233181Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c miceMaryam HomayoonYahya TahamtanMohammad KargarSeyed Mohammad Hossein HosseiniAbbas Akhavan SepahyObjective: To evaluate the effects of Pasteurella multocida (P. multocida) vaccines on the expression and release of antibodies, interleukin (IL)-6 and IL-12 by serum. Methods: Balb/c mice were immunized with two formalin and iron inactivated vaccine doses within 2 weeks. The vaccines were adjuvant with P. multocida A strain, P. multocida B strain and Salmonella typhimurium bacterial DNA (AbDNA, BbDNA and SbDNA for short, respectively). The animals were challenged 4 weeks after immunization. Blood of mice was collected to detect the change of specific antibody, IL-6, and IL-12 using ELISA. Results: The specific antibody and interleukins in the immunized group increased significantly compared to the control mice after vaccination and challenge (P<0.05). The highest release of these cytokines was obtained by P. multocida inactivated with iron and adjuvant with AbDNA at a concentration of 25 μg/mL. The antibody titer peak was 0.447 in mice vaccinated with iron-killed whole-cell antigen adjunct with AbDNA. The time-courses of release showed that bacterial DNA was able to stimulate IL-6 and IL-12 production more than alum (P<0.05). Conclusions: Our findings introduce that bacterial DNA is capable of releasing an immunological response with several cytokines. These indicate that bacterial DNA entrapped with killed P. multocida antigen is a new and effective adjuvant to enhance specific immunity and resistance of animal against the infectious pathogen, which could simplify the development of highly promising strong adjuvant.http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=5;spage=336;epage=341;aulast=Homayoonpasteurella multocidabacterial dnaadjuvant activityvaccineimmunitybalb/c mice |
| spellingShingle | Maryam Homayoon Yahya Tahamtan Mohammad Kargar Seyed Mohammad Hossein Hosseini Abbas Akhavan Sepahy Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice Asian Pacific Journal of Tropical Medicine pasteurella multocida bacterial dna adjuvant activity vaccine immunity balb/c mice |
| title | Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice |
| title_full | Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice |
| title_fullStr | Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice |
| title_full_unstemmed | Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice |
| title_short | Adjuvant activity of Pasteurella multocida A strain, Pasteurella multocida B strain and Salmonella typhimurium bacterial DNA on cellular and humoral immunity responses against Pasteurella multocida specific strain infections in Balb/c mice |
| title_sort | adjuvant activity of pasteurella multocida a strain pasteurella multocida b strain and salmonella typhimurium bacterial dna on cellular and humoral immunity responses against pasteurella multocida specific strain infections in balb c mice |
| topic | pasteurella multocida bacterial dna adjuvant activity vaccine immunity balb/c mice |
| url | http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=5;spage=336;epage=341;aulast=Homayoon |
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