Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)
<b>Background:</b> Magnesium (Mg) is one of the most promising materials for human use in surgery due to material characteristics such as its elastic modulus as well as its resorbable and regenerative properties. In this study, HF-coated and uncoated novel bioresorbable magnesium fixatio...
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MDPI AG
2021-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/22/12567 |
| _version_ | 1797509909558853632 |
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| author | Ole Jung Bernhard Hesse Sanja Stojanovic Christian Seim Timm Weitkamp Milijana Batinic Oliver Goerke Željka Perić Kačarević Patrick Rider Stevo Najman Mike Barbeck |
| author_facet | Ole Jung Bernhard Hesse Sanja Stojanovic Christian Seim Timm Weitkamp Milijana Batinic Oliver Goerke Željka Perić Kačarević Patrick Rider Stevo Najman Mike Barbeck |
| author_sort | Ole Jung |
| collection | DOAJ |
| description | <b>Background:</b> Magnesium (Mg) is one of the most promising materials for human use in surgery due to material characteristics such as its elastic modulus as well as its resorbable and regenerative properties. In this study, HF-coated and uncoated novel bioresorbable magnesium fixation screws for maxillofacial and dental surgical applications were investigated in vitro and in vivo to evaluate the biocompatibility of the HF coating. <b>Methods:</b> Mg alloy screws that had either undergone a surface treatment with hydrofluoric-acid (HF) or left untreated were investigated. In vitro investigation included XTT, BrdU and LDH in accordance with the DIN ISO 10993-5/-12. In vivo, the screws were implanted into the tibia of rabbits. After 3 and 6 weeks, degradation, local tissue reactions and bony integration were analyzed histopathologically and histomorphometrically. Additionally, SEM/EDX analysis and synchrotron phase-contrast microtomography (µCT) measurements were conducted. The in vitro analyses revealed that the Mg screws are cytocompatible, with improved results when the surface had been passivated with HF. In vivo, the HF-treated Mg screws implanted showed a reduction in gas formation, slower biodegradation and a better bony integration in comparison to the untreated Mg screws. Histopathologically, the HF-passivated screws induced a layer of macrophages as part of its biodegradation process, whereas the untreated screws caused a slight fibrous tissue reaction. SEM/EDX analysis showed that both screws formed a similar layer of calcium phosphates on their surfaces and were surrounded by bone. Furthermore, the µCT revealed the presence of a metallic core of the screws, a faster absorbing corrosion front and a slow absorbing region of corroded magnesium. <b>Conclusions:</b> Overall, the HF-passivated Mg fixation screws showed significantly better biocompatibility in vitro and in vivo compared to the untreated screws. |
| first_indexed | 2024-03-10T05:24:24Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T05:24:24Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2364fcc73cd44ba18d40530fc8ae319e2023-11-22T23:44:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-11-0122221256710.3390/ijms222212567Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR)Ole Jung0Bernhard Hesse1Sanja Stojanovic2Christian Seim3Timm Weitkamp4Milijana Batinic5Oliver Goerke6Željka Perić Kačarević7Patrick Rider8Stevo Najman9Mike Barbeck10Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, 18057 Rostock, GermanyXploraytion GmbH, 10625 Berlin, GermanyDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, 18108 Niš, SerbiaXploraytion GmbH, 10625 Berlin, GermanySynchrotron SOLEIL, Gif-sur-Yvette, 91190 Saint-Aubin, FranceDepartment of Ceramic Materials, Chair of Advanced Ceramic Materials, Institute for Materials Science and Technologies, Technical University of Berlin, 10623 Berlin, GermanyDepartment of Ceramic Materials, Chair of Advanced Ceramic Materials, Institute for Materials Science and Technologies, Technical University of Berlin, 10623 Berlin, GermanyDepartment of Anatomy Histology, Embryology, Pathology Anatomy and Pathology Histology, Faculty of Dental Medicine and Health, University of Osijek, 31000 Osijek, CroatiaBerlinAnalytix GmbH, 12109 Berlin, GermanyDepartment of Biology and Human Genetics, Faculty of Medicine, University of Niš, 18108 Niš, SerbiaBerlinAnalytix GmbH, 12109 Berlin, Germany<b>Background:</b> Magnesium (Mg) is one of the most promising materials for human use in surgery due to material characteristics such as its elastic modulus as well as its resorbable and regenerative properties. In this study, HF-coated and uncoated novel bioresorbable magnesium fixation screws for maxillofacial and dental surgical applications were investigated in vitro and in vivo to evaluate the biocompatibility of the HF coating. <b>Methods:</b> Mg alloy screws that had either undergone a surface treatment with hydrofluoric-acid (HF) or left untreated were investigated. In vitro investigation included XTT, BrdU and LDH in accordance with the DIN ISO 10993-5/-12. In vivo, the screws were implanted into the tibia of rabbits. After 3 and 6 weeks, degradation, local tissue reactions and bony integration were analyzed histopathologically and histomorphometrically. Additionally, SEM/EDX analysis and synchrotron phase-contrast microtomography (µCT) measurements were conducted. The in vitro analyses revealed that the Mg screws are cytocompatible, with improved results when the surface had been passivated with HF. In vivo, the HF-treated Mg screws implanted showed a reduction in gas formation, slower biodegradation and a better bony integration in comparison to the untreated Mg screws. Histopathologically, the HF-passivated screws induced a layer of macrophages as part of its biodegradation process, whereas the untreated screws caused a slight fibrous tissue reaction. SEM/EDX analysis showed that both screws formed a similar layer of calcium phosphates on their surfaces and were surrounded by bone. Furthermore, the µCT revealed the presence of a metallic core of the screws, a faster absorbing corrosion front and a slow absorbing region of corroded magnesium. <b>Conclusions:</b> Overall, the HF-passivated Mg fixation screws showed significantly better biocompatibility in vitro and in vivo compared to the untreated screws.https://www.mdpi.com/1422-0067/22/22/12567guided bone regeneration (GBR)barrier membranefixationscrewpinbiodegradation |
| spellingShingle | Ole Jung Bernhard Hesse Sanja Stojanovic Christian Seim Timm Weitkamp Milijana Batinic Oliver Goerke Željka Perić Kačarević Patrick Rider Stevo Najman Mike Barbeck Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR) International Journal of Molecular Sciences guided bone regeneration (GBR) barrier membrane fixation screw pin biodegradation |
| title | Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR) |
| title_full | Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR) |
| title_fullStr | Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR) |
| title_full_unstemmed | Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR) |
| title_short | Biocompatibility Analyses of HF-Passivated Magnesium Screws for Guided Bone Regeneration (GBR) |
| title_sort | biocompatibility analyses of hf passivated magnesium screws for guided bone regeneration gbr |
| topic | guided bone regeneration (GBR) barrier membrane fixation screw pin biodegradation |
| url | https://www.mdpi.com/1422-0067/22/22/12567 |
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