Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants
This work aimed to evaluate the valorisation of the volatile oil of "Rosmarinus officinalis L.", a spontaneously growing medicinal plant in Tunisia, by studying its chemical composition, anthelmintic and anticoccidial potentials against Eimeria spp. and Haemonchus contortus at different es...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2021-04-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-202104-0003_essential-oil-of-8239-rosmarinus-officinalis-induces-in-8239-vitro-anthelmintic-and-anticoccidial-effects-aga.php |
| _version_ | 1797897424007593984 |
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| author | M Aouadi E Sebai A Saratsis V Kantzoura K Saratsi K Msaada S Sotiraki H Akkari |
| author_facet | M Aouadi E Sebai A Saratsis V Kantzoura K Saratsi K Msaada S Sotiraki H Akkari |
| author_sort | M Aouadi |
| collection | DOAJ |
| description | This work aimed to evaluate the valorisation of the volatile oil of "Rosmarinus officinalis L.", a spontaneously growing medicinal plant in Tunisia, by studying its chemical composition, anthelmintic and anticoccidial potentials against Eimeria spp. and Haemonchus contortus at different essential oil concentrations. The main compounds of the R. officinalis essential oil identified by GC/MS were three monoterpenes: 1,8-cineole (52.06%), α-pinene (15.35%) and camphor (7.69%). The anticoccidial activity was estimated by the inhibition percentage of the oocyte sporulation in addition to the unsporulated and degenerated Eimeria oocysts using a haemocytometer after exposure to different essential oil concentrations. The essential oil was active against Eimeria spp. oocysts of sheep at IC50 = 1.82 ug/ml. Therefore, the IC50 values of the anticoccidial activity of this oil examined was 1.82 mg/ml. The anthelmintic efficacy of the rosemary volatile oil against Haemonchus contortus was realised by two in vitro tests: the egg hatch assay (EHA) and the adult worm's motility assay (AWMA), by comparing this efficacy with albendazole (anthelmintic, of reference). In the egg hatch assay, the percentage of inhibition was observed at 16 mg/ml and was 73.76% after 2 days of incubation (IC50 = 11.41 mg/ml) and for the adult worm's motility assay, it was 100% inhibition. |
| first_indexed | 2024-04-10T07:57:15Z |
| format | Article |
| id | doaj.art-2369a011e4584b29848e1420236b46fe |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T07:57:15Z |
| publishDate | 2021-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-2369a011e4584b29848e1420236b46fe2023-02-23T03:51:05ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922021-04-0166414615510.17221/139/2020-VETMEDvet-202104-0003Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminantsM Aouadi0E Sebai1A Saratsis2V Kantzoura3K Saratsi4K Msaada5S Sotiraki6H Akkari7Laboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba, TunisiaLaboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba, TunisiaVeterinary Research Institute, Hellenic Agricultural Organisation - DEMETER Campus Thermi, Thessaloniki, GreeceVeterinary Research Institute, Hellenic Agricultural Organisation - DEMETER Campus Thermi, Thessaloniki, GreeceVeterinary Research Institute, Hellenic Agricultural Organisation - DEMETER Campus Thermi, Thessaloniki, GreeceLaboratory of Aromatic and Medicinal Plants, Biotechnology Center in Borj Cedria Technopole, Hammam Lif, TunisiaVeterinary Research Institute, Hellenic Agricultural Organisation - DEMETER Campus Thermi, Thessaloniki, GreeceLaboratory of Parasitology, National School of Veterinary Medicine of Sidi Thabet, University of Manouba, Manouba, TunisiaThis work aimed to evaluate the valorisation of the volatile oil of "Rosmarinus officinalis L.", a spontaneously growing medicinal plant in Tunisia, by studying its chemical composition, anthelmintic and anticoccidial potentials against Eimeria spp. and Haemonchus contortus at different essential oil concentrations. The main compounds of the R. officinalis essential oil identified by GC/MS were three monoterpenes: 1,8-cineole (52.06%), α-pinene (15.35%) and camphor (7.69%). The anticoccidial activity was estimated by the inhibition percentage of the oocyte sporulation in addition to the unsporulated and degenerated Eimeria oocysts using a haemocytometer after exposure to different essential oil concentrations. The essential oil was active against Eimeria spp. oocysts of sheep at IC50 = 1.82 ug/ml. Therefore, the IC50 values of the anticoccidial activity of this oil examined was 1.82 mg/ml. The anthelmintic efficacy of the rosemary volatile oil against Haemonchus contortus was realised by two in vitro tests: the egg hatch assay (EHA) and the adult worm's motility assay (AWMA), by comparing this efficacy with albendazole (anthelmintic, of reference). In the egg hatch assay, the percentage of inhibition was observed at 16 mg/ml and was 73.76% after 2 days of incubation (IC50 = 11.41 mg/ml) and for the adult worm's motility assay, it was 100% inhibition.https://vetmed.agriculturejournals.cz/artkey/vet-202104-0003_essential-oil-of-8239-rosmarinus-officinalis-induces-in-8239-vitro-anthelmintic-and-anticoccidial-effects-aga.phpcoccidiaeimeria spp.essential oilhaemonchus contortusrosmarinus officinalissmall ruminants |
| spellingShingle | M Aouadi E Sebai A Saratsis V Kantzoura K Saratsi K Msaada S Sotiraki H Akkari Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants Veterinární Medicína coccidia eimeria spp. essential oil haemonchus contortus rosmarinus officinalis small ruminants |
| title | Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants |
| title_full | Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants |
| title_fullStr | Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants |
| title_full_unstemmed | Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants |
| title_short | Essential oil of Rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against Haemonchus contortus and Eimeria spp. in small ruminants |
| title_sort | essential oil of rosmarinus officinalis induces in vitro anthelmintic and anticoccidial effects against haemonchus contortus and eimeria spp in small ruminants |
| topic | coccidia eimeria spp. essential oil haemonchus contortus rosmarinus officinalis small ruminants |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-202104-0003_essential-oil-of-8239-rosmarinus-officinalis-induces-in-8239-vitro-anthelmintic-and-anticoccidial-effects-aga.php |
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