A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo
Abstract Background Ag2S has the characteristics of conventional quantum dot such as broad excitation spectrum, narrow emission spectrum, long fluorescence lifetime, strong anti-bleaching ability, and other optical properties. Moreover, since its fluorescence emission is located in the NIR-II region...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-018-0367-9 |
| _version_ | 1811300488194293760 |
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| author | Xiao-Shuai Zhang Yang Xuan Xiao-Quan Yang Kai Cheng Ruo-Yun Zhang Cheng Li Fang Tan Yuan-Cheng Cao Xian-Lin Song Jie An Xiao-Lin Hou Yuan-Di Zhao |
| author_facet | Xiao-Shuai Zhang Yang Xuan Xiao-Quan Yang Kai Cheng Ruo-Yun Zhang Cheng Li Fang Tan Yuan-Cheng Cao Xian-Lin Song Jie An Xiao-Lin Hou Yuan-Di Zhao |
| author_sort | Xiao-Shuai Zhang |
| collection | DOAJ |
| description | Abstract Background Ag2S has the characteristics of conventional quantum dot such as broad excitation spectrum, narrow emission spectrum, long fluorescence lifetime, strong anti-bleaching ability, and other optical properties. Moreover, since its fluorescence emission is located in the NIR-II region, has stronger penetrating ability for tissue. Ag2S quantum dot has strong absorption during the visible and NIR regions, it has good photothermal and photoacoustic response under certain wavelength excitation. Results 200 nm aqueous probe Ag2S@DSPE-PEG2000-FA (Ag2S@DP-FA) with good dispersibility and stability was prepared by coating hydrophobic Ag2S with the mixture of folic acid (FA) modified DSPE-PEG2000 (DP) and other polymers, it was found the probe had good fluorescent, photoacoustic and photothermal responses, and a low cell cytotoxicity at 50 μg/mL Ag concentration. Blood biochemical analysis, liver enzyme and tissue histopathological test showed that no significant influence was observed on blood and organs within 15 days after injection of the probe. In vivo and in vitro fluorescence and photoacoustic imaging of the probe further demonstrated that the Ag2S@DP-FA probe had good active targeting ability for tumor. In vivo and in vitro photothermal therapy experiments confirmed that the probe also had good ability of killing tumor by photothermal. Conclusions Ag2S@DP-FA was a safe, integrated diagnosis and treatment probe with multi-mode imaging, photothermal therapy and active targeting ability, which had a great application prospect in the early diagnosis and treatment of tumor. |
| first_indexed | 2024-04-13T06:51:56Z |
| format | Article |
| id | doaj.art-236c9c1f66ab4256b98bc22d7a53d71b |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-13T06:51:56Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-236c9c1f66ab4256b98bc22d7a53d71b2022-12-22T02:57:22ZengBMCJournal of Nanobiotechnology1477-31552018-04-0116111510.1186/s12951-018-0367-9A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivoXiao-Shuai Zhang0Yang Xuan1Xiao-Quan Yang2Kai Cheng3Ruo-Yun Zhang4Cheng Li5Fang Tan6Yuan-Cheng Cao7Xian-Lin Song8Jie An9Xiao-Lin Hou10Yuan-Di Zhao11Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyKey Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, Jianghan UniversityKey Laboratory of Optoelectronic Chemical Materials and Devices, Ministry of Education, Jianghan UniversityBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyBritton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics–Hubei Bioinformatics & Molecular Imaging Key Laboratory, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and TechnologyAbstract Background Ag2S has the characteristics of conventional quantum dot such as broad excitation spectrum, narrow emission spectrum, long fluorescence lifetime, strong anti-bleaching ability, and other optical properties. Moreover, since its fluorescence emission is located in the NIR-II region, has stronger penetrating ability for tissue. Ag2S quantum dot has strong absorption during the visible and NIR regions, it has good photothermal and photoacoustic response under certain wavelength excitation. Results 200 nm aqueous probe Ag2S@DSPE-PEG2000-FA (Ag2S@DP-FA) with good dispersibility and stability was prepared by coating hydrophobic Ag2S with the mixture of folic acid (FA) modified DSPE-PEG2000 (DP) and other polymers, it was found the probe had good fluorescent, photoacoustic and photothermal responses, and a low cell cytotoxicity at 50 μg/mL Ag concentration. Blood biochemical analysis, liver enzyme and tissue histopathological test showed that no significant influence was observed on blood and organs within 15 days after injection of the probe. In vivo and in vitro fluorescence and photoacoustic imaging of the probe further demonstrated that the Ag2S@DP-FA probe had good active targeting ability for tumor. In vivo and in vitro photothermal therapy experiments confirmed that the probe also had good ability of killing tumor by photothermal. Conclusions Ag2S@DP-FA was a safe, integrated diagnosis and treatment probe with multi-mode imaging, photothermal therapy and active targeting ability, which had a great application prospect in the early diagnosis and treatment of tumor.http://link.springer.com/article/10.1186/s12951-018-0367-9Ag2SCancer active targetingFluorescence imagingPhotoacoustic imagingPhotothermal therapy |
| spellingShingle | Xiao-Shuai Zhang Yang Xuan Xiao-Quan Yang Kai Cheng Ruo-Yun Zhang Cheng Li Fang Tan Yuan-Cheng Cao Xian-Lin Song Jie An Xiao-Lin Hou Yuan-Di Zhao A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo Journal of Nanobiotechnology Ag2S Cancer active targeting Fluorescence imaging Photoacoustic imaging Photothermal therapy |
| title | A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo |
| title_full | A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo |
| title_fullStr | A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo |
| title_full_unstemmed | A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo |
| title_short | A multifunctional targeting probe with dual-mode imaging and photothermal therapy used in vivo |
| title_sort | multifunctional targeting probe with dual mode imaging and photothermal therapy used in vivo |
| topic | Ag2S Cancer active targeting Fluorescence imaging Photoacoustic imaging Photothermal therapy |
| url | http://link.springer.com/article/10.1186/s12951-018-0367-9 |
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