circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression
Summary: Many circular RNAs (circRNAs) have been identified to be associated with hepatocellular carcinoma (HCC) progression. We aim to explore the diagnostic potential, functions, and mechanism of circELMOD3 in HCC. Differentially expressed circRNAs in HCC and its paired adjacent tissues were ident...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223018953 |
| _version_ | 1797647802673659904 |
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| author | Mingshuang Lai Meiliang Liu Deyuan Li Ruirui Zhang Lijun Wang Xiaofei Li Sixian Chen Siqian Wu Suyang Xiao Liling Wei Xiaogang Wang Aruo Nan Xiaoyun Zeng |
| author_facet | Mingshuang Lai Meiliang Liu Deyuan Li Ruirui Zhang Lijun Wang Xiaofei Li Sixian Chen Siqian Wu Suyang Xiao Liling Wei Xiaogang Wang Aruo Nan Xiaoyun Zeng |
| author_sort | Mingshuang Lai |
| collection | DOAJ |
| description | Summary: Many circular RNAs (circRNAs) have been identified to be associated with hepatocellular carcinoma (HCC) progression. We aim to explore the diagnostic potential, functions, and mechanism of circELMOD3 in HCC. Differentially expressed circRNAs in HCC and its paired adjacent tissues were identified by RNA sequencing. circELMOD3 was downregulated in HCC tissues and was related to clinicopathological characteristics of HCC patients. Additionally, plasma circELMOD3 was shown to be a highly sensitive and non-invasive biomarker to distinguish HCC from healthy controls. Functional assays showed that circELMOD3 inhibited proliferation and induced apoptosis of HCC cells both in vitro and in vivo. Mechanistically, RNA antisense purification (RAP) and luciferase reporter assays verified that circELMOD3 functioned as a sponge for miR-6864-5p leading to increased expression of its target gene TRIM13. Interestingly, RNA stability test demonstrated that circELMOD3 overexpression led to enhanced stability of its directly bound TRIM13 mRNA, which in turn co-activated the p53 signaling pathway. |
| first_indexed | 2024-03-11T15:22:52Z |
| format | Article |
| id | doaj.art-236eb7cc7cc04dc3becc988f5bd29109 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-11T15:22:52Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-236eb7cc7cc04dc3becc988f5bd291092023-10-28T05:08:38ZengElsevieriScience2589-00422023-10-012610107818circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progressionMingshuang Lai0Meiliang Liu1Deyuan Li2Ruirui Zhang3Lijun Wang4Xiaofei Li5Sixian Chen6Siqian Wu7Suyang Xiao8Liling Wei9Xiaogang Wang10Aruo Nan11Xiaoyun Zeng12School of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, ChinaSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, China; Corresponding authorSchool of Public Health, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, China; Guangxi Colleges and Universities Key Laboratory of Prevention and Control of Highly Prevalent Diseases, Guangxi Medical University, Nanning 530021, Guangxi, China; Key Laboratory of Early Prevention and Treatment for Regional High Frequency Tumor (Guangxi Medical University), Ministry of Education, Nanning, Guangxi, China; Corresponding authorSummary: Many circular RNAs (circRNAs) have been identified to be associated with hepatocellular carcinoma (HCC) progression. We aim to explore the diagnostic potential, functions, and mechanism of circELMOD3 in HCC. Differentially expressed circRNAs in HCC and its paired adjacent tissues were identified by RNA sequencing. circELMOD3 was downregulated in HCC tissues and was related to clinicopathological characteristics of HCC patients. Additionally, plasma circELMOD3 was shown to be a highly sensitive and non-invasive biomarker to distinguish HCC from healthy controls. Functional assays showed that circELMOD3 inhibited proliferation and induced apoptosis of HCC cells both in vitro and in vivo. Mechanistically, RNA antisense purification (RAP) and luciferase reporter assays verified that circELMOD3 functioned as a sponge for miR-6864-5p leading to increased expression of its target gene TRIM13. Interestingly, RNA stability test demonstrated that circELMOD3 overexpression led to enhanced stability of its directly bound TRIM13 mRNA, which in turn co-activated the p53 signaling pathway.http://www.sciencedirect.com/science/article/pii/S2589004223018953Biological sciencesCancerMolecular biology |
| spellingShingle | Mingshuang Lai Meiliang Liu Deyuan Li Ruirui Zhang Lijun Wang Xiaofei Li Sixian Chen Siqian Wu Suyang Xiao Liling Wei Xiaogang Wang Aruo Nan Xiaoyun Zeng circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression iScience Biological sciences Cancer Molecular biology |
| title | circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression |
| title_full | circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression |
| title_fullStr | circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression |
| title_full_unstemmed | circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression |
| title_short | circELMOD3 increases and stabilizes TRIM13 by sponging miR-6864-5p and direct binding to inhibit HCC progression |
| title_sort | circelmod3 increases and stabilizes trim13 by sponging mir 6864 5p and direct binding to inhibit hcc progression |
| topic | Biological sciences Cancer Molecular biology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223018953 |
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