Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides
Chicken muscle yield and amino acid composition improvements with medium-chain monoglyceride (MG) supplementation were reported by previous studies, but the underlying mechanism was uncertain. This study aimed to decipher chicken amino acid improvements induced by medium-chain monoglycerides in the...
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MDPI AG
2023-01-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/208 |
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| author | Tao Liu Shengyue Ruan Qiufen Mo Minjie Zhao Jing Wang Zhangying Ye Li Chen Fengqin Feng |
| author_facet | Tao Liu Shengyue Ruan Qiufen Mo Minjie Zhao Jing Wang Zhangying Ye Li Chen Fengqin Feng |
| author_sort | Tao Liu |
| collection | DOAJ |
| description | Chicken muscle yield and amino acid composition improvements with medium-chain monoglyceride (MG) supplementation were reported by previous studies, but the underlying mechanism was uncertain. This study aimed to decipher chicken amino acid improvements induced by medium-chain monoglycerides in the views of metabolomics, gene expression, and the gut microbiome. Newly hatched chicks (12,000 chicks) were weighed and randomly divided into two flocks, each with six replicates (1000 chicks per replicate), and fed a basal diet (the control group, CON) or a basal diet enriched with 300 mg/kg MG (the treated group, MG). Results demonstrated that MGs significantly increased the chicken flavor and essential and total amino acids. The serum amino acids and derivatives (betaine, <span style="font-variant: small-caps;">l</span>-leucine, <span style="font-variant: small-caps;">l</span>-glutamine, 1-methylhistide), as well as amino acid metabolism pathways in chickens, were enhanced by MG supplementation. Gene expression analysis exhibited that dietary MGs could improve muscle protein synthesis and cell growth via the mTOR/S6K1 pathway. Dietary MGs enhanced the cecal amino acid metabolism by selectively increasing the proportion of genera <i>Lachnospiraceae_NK4A136_group</i> and <i>Bacteroides</i>. Conclusively, the present study demonstrated that dietary MGs improved chicken amino acid composition via increasing both gut amino acid utilization and muscle amino acid deposition. |
| first_indexed | 2024-03-11T08:25:57Z |
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| id | doaj.art-236ecad28e114bd4aa3284f7568a9744 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:25:57Z |
| publishDate | 2023-01-01 |
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| series | Metabolites |
| spelling | doaj.art-236ecad28e114bd4aa3284f7568a97442023-11-16T22:04:24ZengMDPI AGMetabolites2218-19892023-01-0113220810.3390/metabo13020208Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain MonoglyceridesTao Liu0Shengyue Ruan1Qiufen Mo2Minjie Zhao3Jing Wang4Zhangying Ye5Li Chen6Fengqin Feng7College of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaCollege of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaThe Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, ChinaChicken muscle yield and amino acid composition improvements with medium-chain monoglyceride (MG) supplementation were reported by previous studies, but the underlying mechanism was uncertain. This study aimed to decipher chicken amino acid improvements induced by medium-chain monoglycerides in the views of metabolomics, gene expression, and the gut microbiome. Newly hatched chicks (12,000 chicks) were weighed and randomly divided into two flocks, each with six replicates (1000 chicks per replicate), and fed a basal diet (the control group, CON) or a basal diet enriched with 300 mg/kg MG (the treated group, MG). Results demonstrated that MGs significantly increased the chicken flavor and essential and total amino acids. The serum amino acids and derivatives (betaine, <span style="font-variant: small-caps;">l</span>-leucine, <span style="font-variant: small-caps;">l</span>-glutamine, 1-methylhistide), as well as amino acid metabolism pathways in chickens, were enhanced by MG supplementation. Gene expression analysis exhibited that dietary MGs could improve muscle protein synthesis and cell growth via the mTOR/S6K1 pathway. Dietary MGs enhanced the cecal amino acid metabolism by selectively increasing the proportion of genera <i>Lachnospiraceae_NK4A136_group</i> and <i>Bacteroides</i>. Conclusively, the present study demonstrated that dietary MGs improved chicken amino acid composition via increasing both gut amino acid utilization and muscle amino acid deposition.https://www.mdpi.com/2218-1989/13/2/208chicken amino acidmedium-chain monoglyceridesbroiler chickensserum metabolomegut microbiome |
| spellingShingle | Tao Liu Shengyue Ruan Qiufen Mo Minjie Zhao Jing Wang Zhangying Ye Li Chen Fengqin Feng Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides Metabolites chicken amino acid medium-chain monoglycerides broiler chickens serum metabolome gut microbiome |
| title | Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides |
| title_full | Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides |
| title_fullStr | Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides |
| title_full_unstemmed | Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides |
| title_short | Integrated Serum Metabolome and Gut Microbiome to Decipher Chicken Amino Acid Improvements Induced by Medium-Chain Monoglycerides |
| title_sort | integrated serum metabolome and gut microbiome to decipher chicken amino acid improvements induced by medium chain monoglycerides |
| topic | chicken amino acid medium-chain monoglycerides broiler chickens serum metabolome gut microbiome |
| url | https://www.mdpi.com/2218-1989/13/2/208 |
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