Scleral ultrastructure and biomechanical changes in rabbits after negative lens application
AIM: To address the microstructure and biomechanical changes of the sclera of rabbits after negative lens application by spectacle frame apparatus. METHODS: Five New Zealand rabbits of seven weeks post-natal were treated with -8 D lens monocularly over the course of two weeks. Refractive errors and...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2018-03-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2018/3/20180302.pdf |
| _version_ | 1811265417191096320 |
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| author | Xiao Lin Bing-Jie Wang Yen-Chiao Wang Ren-Yuan Chu Jin-Hui Dai Xing-Tao Zhou Xiao-Mei Qu Hong Liu Hao Zhou |
| author_facet | Xiao Lin Bing-Jie Wang Yen-Chiao Wang Ren-Yuan Chu Jin-Hui Dai Xing-Tao Zhou Xiao-Mei Qu Hong Liu Hao Zhou |
| author_sort | Xiao Lin |
| collection | DOAJ |
| description | AIM: To address the microstructure and biomechanical changes of the sclera of rabbits after negative lens application by spectacle frame apparatus.
METHODS: Five New Zealand rabbits of seven weeks post-natal were treated with -8 D lens monocularly over the course of two weeks. Refractive errors and axial length (AXL) were measured at the 1st, 7th and 14th days of the induction period. Ultrastructure of sclera was determined with electron microscopy. Biomechanical properties were tested by an Instron 5565 universal testing machine.
RESULTS: Lens-induced (LI) eyes elongated more rapidly compared with fellow eyes with AXL values of 15.56±0.14 and 15.21±0.14 mm (P<0.01). Fibril diameter was significantly smaller in the LI eyes compared with control ones in the inner, middle, and outer layers (inner layer, 63.533 vs 76.467 nm; middle layer, 92.647 vs 123.984 nm; outer layer, 86.999 vs 134.257 nm, P<0.01, respectively). In comparison with control eyes, macrophage-like cells that engulfed fibroblasts, dilated endoplasmic reticulum, and vacuoles in fibroblasts were observed in the inner and middle stroma in the LI eyes. Ultimate stress and Young’s modulus were lower in the LI eyes compared with those in the control eyes.
CONCLUSION: Negative lens application alters eye growth, and results in axial elongation with changes in scleral ultrastructural and mechanical properties. |
| first_indexed | 2024-04-12T20:23:44Z |
| format | Article |
| id | doaj.art-236f205e53b446c599442ba914b85515 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-12T20:23:44Z |
| publishDate | 2018-03-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-236f205e53b446c599442ba914b855152022-12-22T03:17:56ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982018-03-0111335436210.18240/ijo.2018.03.02Scleral ultrastructure and biomechanical changes in rabbits after negative lens applicationXiao Lin0Bing-Jie Wang1Yen-Chiao Wang2Ren-Yuan Chu3Jin-Hui Dai4Xing-Tao Zhou5Xiao-Mei Qu6Hong Liu7Hao Zhou8Department of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaSchool of Optometry, Indiana University, Bloomington, Indiana 47405, United StatesDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaDepartment of Ophthalmology & Visual Science, Eye & ENT Hospital, Shanghai Medical College, Fudan University, Shanghai 200031, China; Key Laboratory of Myopia, Ministry of Health, Fudan University, Shanghai 200031, China; Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai 200031, ChinaAIM: To address the microstructure and biomechanical changes of the sclera of rabbits after negative lens application by spectacle frame apparatus. METHODS: Five New Zealand rabbits of seven weeks post-natal were treated with -8 D lens monocularly over the course of two weeks. Refractive errors and axial length (AXL) were measured at the 1st, 7th and 14th days of the induction period. Ultrastructure of sclera was determined with electron microscopy. Biomechanical properties were tested by an Instron 5565 universal testing machine. RESULTS: Lens-induced (LI) eyes elongated more rapidly compared with fellow eyes with AXL values of 15.56±0.14 and 15.21±0.14 mm (P<0.01). Fibril diameter was significantly smaller in the LI eyes compared with control ones in the inner, middle, and outer layers (inner layer, 63.533 vs 76.467 nm; middle layer, 92.647 vs 123.984 nm; outer layer, 86.999 vs 134.257 nm, P<0.01, respectively). In comparison with control eyes, macrophage-like cells that engulfed fibroblasts, dilated endoplasmic reticulum, and vacuoles in fibroblasts were observed in the inner and middle stroma in the LI eyes. Ultimate stress and Young’s modulus were lower in the LI eyes compared with those in the control eyes. CONCLUSION: Negative lens application alters eye growth, and results in axial elongation with changes in scleral ultrastructural and mechanical properties.http://www.ijo.cn/en_publish/2018/3/20180302.pdf362negative lensrabbitbiomechanicsscleraultrastructure |
| spellingShingle | Xiao Lin Bing-Jie Wang Yen-Chiao Wang Ren-Yuan Chu Jin-Hui Dai Xing-Tao Zhou Xiao-Mei Qu Hong Liu Hao Zhou Scleral ultrastructure and biomechanical changes in rabbits after negative lens application International Journal of Ophthalmology 362 negative lens rabbit biomechanics sclera ultrastructure |
| title | Scleral ultrastructure and biomechanical changes in rabbits after negative lens application |
| title_full | Scleral ultrastructure and biomechanical changes in rabbits after negative lens application |
| title_fullStr | Scleral ultrastructure and biomechanical changes in rabbits after negative lens application |
| title_full_unstemmed | Scleral ultrastructure and biomechanical changes in rabbits after negative lens application |
| title_short | Scleral ultrastructure and biomechanical changes in rabbits after negative lens application |
| title_sort | scleral ultrastructure and biomechanical changes in rabbits after negative lens application |
| topic | 362 negative lens rabbit biomechanics sclera ultrastructure |
| url | http://www.ijo.cn/en_publish/2018/3/20180302.pdf |
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