Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis
Abstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease that develops due to the impaired immune response in genetically susceptible individuals, and its etiopathogenesis is not fully elucidated. IL-17 A is a cytokine that is produced by a type of immune cell called Th17...
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | BMC Gastroenterology |
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| Online Access: | https://doi.org/10.1186/s12876-023-02985-z |
| _version_ | 1797452425054912512 |
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| author | Tahir Buran Muhammet Burak Batır Fethi Sırrı Çam Elmas Kasap Fatih Çöllü Hamide Betül Gerik Çelebi Mustafa Şahin |
| author_facet | Tahir Buran Muhammet Burak Batır Fethi Sırrı Çam Elmas Kasap Fatih Çöllü Hamide Betül Gerik Çelebi Mustafa Şahin |
| author_sort | Tahir Buran |
| collection | DOAJ |
| description | Abstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease that develops due to the impaired immune response in genetically susceptible individuals, and its etiopathogenesis is not fully elucidated. IL-17 A is a cytokine that is produced by a type of immune cell called Th17 cells and is involved in the immune response and inflammation. On the other hand, ADAMTS-1, -4, and − 5 are enzymes that are involved in the breakdown of extracellular matrix proteins, including proteoglycans, which are important components of the intestinal wall. This study aimed to evaluate the relationship between interleukin 17 (IL-17 A) cytokine, which plays a role in the pathogenesis of ulcerative colitis, and the inflammation-controlled a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1, -4, and − 5 protein members. Methods Bowel tissue samples and blood serum from 51 patients with UC and 51 healthy controls were included in this study. mRNA expression levels of the ADAMTS-1, -4, -5, and IL-17 A were analyzed by RT-qPCR, and immunohistochemical analyses were performed to evaluate ADAMTS-1, -4, -5, and IL-17 A proteins in tissue samples. In addition, ELISA analysis determined serum levels of the ADAMTS-1, -4, -5, and IL-17 A. Results RT-qPCR results reveal that the expression of ADAMTS-1, -4, -5, and IL-17 A genes in the UC tissue samples were significantly high according to the control tissue samples. Also, ADAMTS-1, -4, -5, and IL-17 A proteins revealed enhanced expression pattern UC groups according to the control. Also, ADAMTS-1, -4, -5, and IL-17 A protein showed cytoplasmic localization patterns in both control and UC groups. The serum levels of ADAMTS-1,-5, and IL-17 A were significantly higher in UC samples than in the control group. Conclusions We observed a positive correlation between the ADAMTS-1, -5 and IL17A cytokine expression in UC samples. These results provide a new understanding of controlling crucial ADAMTS family protein members by IL-17 A cytokines with UC. |
| first_indexed | 2024-03-09T15:08:34Z |
| format | Article |
| id | doaj.art-23725d0c5a9246c3b3c2f9016e508b94 |
| institution | Directory Open Access Journal |
| issn | 1471-230X |
| language | English |
| last_indexed | 2024-03-09T15:08:34Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Gastroenterology |
| spelling | doaj.art-23725d0c5a9246c3b3c2f9016e508b942023-11-26T13:30:05ZengBMCBMC Gastroenterology1471-230X2023-10-0123111010.1186/s12876-023-02985-zMolecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitisTahir Buran0Muhammet Burak Batır1Fethi Sırrı Çam2Elmas Kasap3Fatih Çöllü4Hamide Betül Gerik Çelebi5Mustafa Şahin6Department of Gastroenterology, Faculty of Medicine, Manisa Celal Bayar UniversityDepartment of Biology, Faculty of Arts and Sciences, Manisa Celal Bayar UniversityDepartment of Medical Genetics, Faculty of Medicine, Manisa Celal Bayar UniversityDepartment of Gastroenterology, Faculty of Medicine, Manisa Celal Bayar UniversityDepartment of Biology, Faculty of Arts and Sciences, Manisa Celal Bayar UniversityDepartment of Medical Genetics, Atatürk City HospitalDepartment of Internal Medicine, Faculty of Medicine, Manisa Celal Bayar UniversityAbstract Background Ulcerative colitis (UC) is a chronic inflammatory bowel disease that develops due to the impaired immune response in genetically susceptible individuals, and its etiopathogenesis is not fully elucidated. IL-17 A is a cytokine that is produced by a type of immune cell called Th17 cells and is involved in the immune response and inflammation. On the other hand, ADAMTS-1, -4, and − 5 are enzymes that are involved in the breakdown of extracellular matrix proteins, including proteoglycans, which are important components of the intestinal wall. This study aimed to evaluate the relationship between interleukin 17 (IL-17 A) cytokine, which plays a role in the pathogenesis of ulcerative colitis, and the inflammation-controlled a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-1, -4, and − 5 protein members. Methods Bowel tissue samples and blood serum from 51 patients with UC and 51 healthy controls were included in this study. mRNA expression levels of the ADAMTS-1, -4, -5, and IL-17 A were analyzed by RT-qPCR, and immunohistochemical analyses were performed to evaluate ADAMTS-1, -4, -5, and IL-17 A proteins in tissue samples. In addition, ELISA analysis determined serum levels of the ADAMTS-1, -4, -5, and IL-17 A. Results RT-qPCR results reveal that the expression of ADAMTS-1, -4, -5, and IL-17 A genes in the UC tissue samples were significantly high according to the control tissue samples. Also, ADAMTS-1, -4, -5, and IL-17 A proteins revealed enhanced expression pattern UC groups according to the control. Also, ADAMTS-1, -4, -5, and IL-17 A protein showed cytoplasmic localization patterns in both control and UC groups. The serum levels of ADAMTS-1,-5, and IL-17 A were significantly higher in UC samples than in the control group. Conclusions We observed a positive correlation between the ADAMTS-1, -5 and IL17A cytokine expression in UC samples. These results provide a new understanding of controlling crucial ADAMTS family protein members by IL-17 A cytokines with UC.https://doi.org/10.1186/s12876-023-02985-zUlcerative colitisIL-17AADAMTSInflammation |
| spellingShingle | Tahir Buran Muhammet Burak Batır Fethi Sırrı Çam Elmas Kasap Fatih Çöllü Hamide Betül Gerik Çelebi Mustafa Şahin Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis BMC Gastroenterology Ulcerative colitis IL-17A ADAMTS Inflammation |
| title | Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis |
| title_full | Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis |
| title_fullStr | Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis |
| title_full_unstemmed | Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis |
| title_short | Molecular analyses of ADAMTS-1, -4, -5, and IL-17 a cytokine relationship in patients with ulcerative colitis |
| title_sort | molecular analyses of adamts 1 4 5 and il 17 a cytokine relationship in patients with ulcerative colitis |
| topic | Ulcerative colitis IL-17A ADAMTS Inflammation |
| url | https://doi.org/10.1186/s12876-023-02985-z |
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